Nested-quantitative PCR approach with improved sensitivity for the detection of low titer levels of Candidatus Liberibacter asiaticus in the Asian citrus psyllid, Diaphorina citri Kuwayama

• Published in: Journal of microbiological methods Vol. 102; pp. 15 - 22
• Main Authors: Coy, M.R., Hoffmann, M., Kingdom Gibbard, H.N., Kuhns, E.H., Pelz-Stelinski, K.S., Stelinski, L.L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2014
• Subjects: Animals; Citrus; Citrus - microbiology; Diaphorina citri; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; False-negatives; Hemiptera - microbiology; Huanglongbing; Humans; Insecta - microbiology; Kuwayama; Low-abundance template; Microbial detection methods; Plant pathogen; Plasmids; Polymerase Chain Reaction - methods; Real-Time Polymerase Chain Reaction - methods; Rhizobiaceae - genetics; Rhizobiaceae - isolation & purification; RNA, Ribosomal, 16S - genetics; Sensitivity and Specificity; Template secondary structure; Template secondary structure; Huanglongbing; Microbial detection methods; Plant pathogen; False-negatives; Low-abundance template

Candidatus Liberibacter asiaticus (CLas) is a phloem-limited bacterium transmitted by the Asian citrus psyllid, Diaphorina citri, and the presumptive causal agent of citrus greening disease. The current method of detection for CLas within plant and insect samples is by a presence/absence qPCR assay...