Phenolic compounds from the stems of Zea mays and their pharmacological activity

• Published in: Applied biological chemistry Vol. 57; no. 3; pp. 379 - 385
• Main Authors: Jung, Ye-Jin, Park, Ji-Hae, Seo, Kyeong-Hwa, Shrestha, Sabina, Lee, Dong-Sung, Kim, Youn-Chul, Kang, Hee-Cheol, Kim, Jiyoung, Baek, Nam-In
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Applied Biological Chemistry 01.06.2014; Springer Nature B.V; 한국응용생명화학회
• Subjects: Apoptosis; Applied Microbiology; Biological Techniques; Bioorganic Chemistry; Cell death; Chemistry; Chemistry and Materials Science; Column chromatography; Glycerol; Lipopolysaccharides; Neuroprotection; NMR; Nuclear magnetic resonance; Oxidative stress; Phenolic compounds; Phenols; 농학; coumarate; diferuloyl glycerol; cornstalk; 1,3; hepatoprotective activity; methyl; methyl ferulate; neuroprotective activity; anti-inflammatory activity
• ISSN: 1738-2203; 2468-0834; 2234-344X; 2468-0842
• DOI: 10.1007/s13765-014-4104-2

The extraction and solvent partition of cornstalks, and repeated column chromatography for EtOAc fraction yielded four phenolic compounds, methyl ( Z )- p -coumarate ( 1 ), methyl ( E )- p -coumarate ( 2 ), methyl ferulate ( 3 ), and 1,3- O -diferuloyl glycerol ( 4 ). The chemical structures were identified on the basis of spectroscopic data analyses including NMR, MS, and IR. All compounds were isolated for the first time from the cornstalks. Compounds 1, 2 , and 4 were evaluated for inhibition activity on NO production in lipopolysaccharide -induced RAW 264.7 cells, neuroprotective activity on glutamate-induced cell death in HT22 cells, and hepatoprotective activity on t -BHP-induced oxidative stress in HepG2 cells. Compounds 2 and 4 showed inhibitory effects on NO production with the IC 50 values of 19.29 and 5.62 μM, respectively, and compound 4 showed protective effect on glutamate-induced cell death and t -BHP-induced oxidative stress with EC 50 values of 19.51 and 71.29 μM, respectively.