Enterohemorrhagic Escherichia coli (EHEC) in water from karst springs: detection with real-time PCR and isolation of strains

• Published in: Journal für Verbraucherschutz und Lebensmittelsicherheit Vol. 11; no. 4; pp. 353 - 357
• Main Authors: Baumgartner, Andreas, Niederhauser, Isabel, Diston, David, Moor, Dominik
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.12.2016; Springer Nature B.V
• Subjects: Agriculture; Announcements and Reports; Biomedical and Life Sciences; Biotechnology; Chemistry/Food Science; Consumer protection; E coli; Escherichia coli; Food Science; Life Sciences; Plant Genetics and Genomics; EHEC; Microbiological criteria; Virulent strains; Official method; Water contamination

Within 2 months, two water sources in a karst area in Switzerland were sampled 9 times each, and analyzed by real-time PCR for 6 EHEC O-types, Shiga-like-toxin ( stx 1 and stx 2) and intimin ( eae ) genes. With the exception of O111, 5 O-types were recorded regularly and at high frequencies (O26: 33...