The major protein in the midgut of teneral Glossina morsitans morsitans is a molecular chaperone from the endosymbiotic bacterium Wigglesworthia glossinidia
Molecules in the midgut of the tsetse fly (Diptera: Glossinidiae) are thought to play an important role in the life cycle of African trypanosomes by influencing their initial establishment in the midgut and subsequent differentiation events that ultimately affect parasite transmission. It is thus im...
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Published in | Insect biochemistry and molecular biology Vol. 32; no. 11; pp. 1429 - 1438 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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England
Elsevier Ltd
01.11.2002
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Abstract | Molecules in the midgut of the tsetse fly (Diptera: Glossinidiae) are thought to play an important role in the life cycle of African trypanosomes by influencing their initial establishment in the midgut and subsequent differentiation events that ultimately affect parasite transmission. It is thus important to determine the molecular composition of the tsetse midgut to aid in understanding disease transmission by these medically important insect vectors. Here, we report that the most abundant protein in the midguts of teneral (unfed)
Glossina morsitans morsitans is a 60 kDa molecular chaperone of bacterial origin. Two species of symbiotic bacteria reside in the tsetse midgut,
Sodalis glossinidius and
Wigglesworthia glossinidia. To determine the exact origin of the 60 kDa molecule, a protein microchemical approach involving two-dimensional (2-D) gel electrophoresis and mass spectrometry was used. Peptide mass maps were compared to virtual peptide maps predicted for
S. glossinidius and
W. glossinidia 60 kDa chaperone sequences. Four signature peptides were identified, revealing that the source of the chaperone was
W. glossinidia. Comparative 2-D gel electrophoresis and immunoblotting further revealed that this protein was localized to the bacteriome and not the distal portion of the tsetse midgut. The possible function of this highly abundant endosymbiont chaperone in the tsetse midgut is discussed. |
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AbstractList | Molecules in the midgut of the tsetse fly (Diptera: Glossinidiae) are thought to play an important role in the life cycle of African trypanosomes by influencing their initial establishment in the midgut and subsequent differentiation events that ultimately affect parasite transmission. It is thus important to determine the molecular composition of the tsetse midgut to aid in understanding disease transmission by these medically important insect vectors. Here, we report that the most abundant protein in the midguts of teneral (unfed) Glossina morsitans morsitans is a 60 kDa molecular chaperone of bacterial origin. Two species of symbiotic bacteria reside in the tsetse midgut, Sodalis glossinidius and Wigglesworthia glossinidia. To determine the exact origin of the 60 kDa molecule, a protein microchemical approach involving two-dimensional (2-D) gel electrophoresis and mass spectrometry was used. Peptide mass maps were compared to virtual peptide maps predicted for S. glossinidius and W. glossinidia 60 kDa chaperone sequences. Four signature peptides were identified, revealing that the source of the chaperone was W. glossinidia. Comparative 2-D gel electrophoresis and immunoblotting further revealed that this protein was localized to the bacteriome and not the distal portion of the tsetse midgut. The possible function of this highly abundant endosymbiont chaperone in the tsetse midgut is discussed. Molecules in the midgut of the tsetse fly (Diptera: Glossinidiae) are thought to play an important role in the life cycle of African trypanosomes by influencing their initial establishment in the midgut and subsequent differentiation events that ultimately affect parasite transmission. It is thus important to determine the molecular composition of the tsetse midgut to aid in understanding disease transmission by these medically important insect vectors. Here, we report that the most abundant protein in the midguts of teneral (unfed) Glossina morsitans morsitans is a 60 kDa molecular chaperone of bacterial origin. Two species of symbiotic bacteria reside in the tsetse midgut, Sodalis glossinidius and Wigglesworthia glossinidia. To determine the exact origin of the 60 kDa molecule, a protein microchemical approach involving two-dimensional (2-D) gel electrophoresis and mass spectrometry was used. Peptide mass maps were compared to virtual peptide maps predicted for S. glossinidius and W. glossinidia 60 kDa chaperone sequences. Four signature peptides were identified, revealing that the source of the chaperone was W. glossinidia. Comparative 2-D gel electrophoresis and immunoblotting further revealed that this protein was localized to the bacteriome and not the distal portion of the tsetse midgut. The possible function of this highly abundant endosymbiont chaperone in the tsetse midgut is discussed. |
Author | Aksoy, S. Pearson, T.W. Gooding, R.H. Haines, L.R. Haddow, J.D. |
Author_xml | – sequence: 1 givenname: L.R. surname: Haines fullname: Haines, L.R. organization: Department of Biochemistry and Microbiology, Box 3055 Petch Building, University of Victoria, Victoria, British Columbia, Canada V8W 3P6 – sequence: 2 givenname: J.D. surname: Haddow fullname: Haddow, J.D. organization: Department of Biochemistry and Microbiology, Box 3055 Petch Building, University of Victoria, Victoria, British Columbia, Canada V8W 3P6 – sequence: 3 givenname: S. surname: Aksoy fullname: Aksoy, S. organization: Department of Epidemiology and Public Health, Section of Vector Biology, Yale University School of Medicine, New Haven, CT 06510, USA – sequence: 4 givenname: R.H. surname: Gooding fullname: Gooding, R.H. organization: Department of Biological Sciences, Biological Sciences Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2E9 – sequence: 5 givenname: T.W. surname: Pearson fullname: Pearson, T.W. email: parasite@uvvm.uvic.ca organization: Department of Biochemistry and Microbiology, Box 3055 Petch Building, University of Victoria, Victoria, British Columbia, Canada V8W 3P6 |
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Keywords | Symbionts Midgut Sodalis glossinidius Wigglesworthia glossinidia Tsetse Mass spectrometry Molecular chaperone |
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SubjectTerms | Amino Acid Sequence Animals Cloning, Molecular Digestive System - chemistry Enterobacteriaceae - physiology Insect Proteins - chemistry Insect Proteins - genetics Insect Proteins - isolation & purification Life Cycle Stages Mass Spectrometry Midgut Molecular chaperone Molecular Chaperones - chemistry Molecular Chaperones - genetics Molecular Chaperones - isolation & purification Molecular Sequence Data Peptide Fragments - chemistry Peptide Fragments - isolation & purification Sequence Alignment Sequence Homology, Amino Acid Sodalis glossinidius Symbionts Symbiosis Tsetse Tsetse Flies - growth & development Tsetse Flies - microbiology Tsetse Flies - physiology Wigglesworthia glossinidia |
Title | The major protein in the midgut of teneral Glossina morsitans morsitans is a molecular chaperone from the endosymbiotic bacterium Wigglesworthia glossinidia |
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