The Farnesyltransferase β-Subunit Ram1 Regulates Sporisorium scitamineum Mating, Pathogenicity and Cell Wall Integrity
The basidiomycetous fungus causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for infection to the host; however, its underlying molecular mechanism has not been fully studied. In this study, we identified a conserved farnesyltransferase (FTase) β su...
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| Published in | Frontiers in microbiology Vol. 10; p. 976 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
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08.05.2019
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| Abstract | The basidiomycetous fungus
causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for infection to the host; however, its underlying molecular mechanism has not been fully studied. In this study, we identified a conserved farnesyltransferase (FTase) β subunit Ram1 in
. The
Δ mutant displayed significantly reduced mating/filamentation, thus of weak pathogenicity to the host cane. The
Δ mutant sporidia showed more tolerant toward cell wall stressor Congo red compared to that of the wild-type. Transcriptional profiling showed that Congo red treatment resulted in notable up-regulation of the core genes involving in cell wall integrity pathway in
Δ sporidia compared with that of WT, indicating that Ram1 may be involved in cell wall integrity regulation. In yeast the heterodimeric FTase is responsible for post-translational modification of Ras (small G protein) and a-factor (pheromone). We also identified and characterized two conserved Ras proteins, Ras1 and Ras2, respectively, and a
pheromone precursor Mfa1. The
Δ,
Δ and
Δ mutants all displayed reduced mating/filamentation similar as the
Δ mutant. However, both
Δ and
Δ mutants were hypersensitive to Congo red while the
Δ mutant was the same as wild-type. Overall our study displayed that
plays an essential role in
mating/filamentation, pathogenicity, and cell wall stability. |
|---|---|
| AbstractList | The basidiomycetous fungus Sporisorium scitamineum causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for infection to the host; however, its underlying molecular mechanism has not been fully studied. In this study, we identified a conserved farnesyltransferase (FTase) β subunit Ram1 in S. scitamineum. The ram1Δ mutant displayed significantly reduced mating/filamentation, thus of weak pathogenicity to the host cane. The ram1Δ mutant sporidia showed more tolerant toward cell wall stressor Congo red compared to that of the wild-type. Transcriptional profiling showed that Congo red treatment resulted in notable up-regulation of the core genes involving in cell wall integrity pathway in ram1Δ sporidia compared with that of WT, indicating that Ram1 may be involved in cell wall integrity regulation. In yeast the heterodimeric FTase is responsible for post-translational modification of Ras (small G protein) and a-factor (pheromone). We also identified and characterized two conserved Ras proteins, Ras1 and Ras2, respectively, and a MAT-1 pheromone precursor Mfa1. The ras1Δ, ras2Δ and mfa1Δ mutants all displayed reduced mating/filamentation similar as the ram1Δ mutant. However, both ras1Δ and ras2Δ mutants were hypersensitive to Congo red while the mfa1Δ mutant was the same as wild-type. Overall our study displayed that RAM1 plays an essential role in S. scitamineum mating/filamentation, pathogenicity, and cell wall stability.The basidiomycetous fungus Sporisorium scitamineum causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for infection to the host; however, its underlying molecular mechanism has not been fully studied. In this study, we identified a conserved farnesyltransferase (FTase) β subunit Ram1 in S. scitamineum. The ram1Δ mutant displayed significantly reduced mating/filamentation, thus of weak pathogenicity to the host cane. The ram1Δ mutant sporidia showed more tolerant toward cell wall stressor Congo red compared to that of the wild-type. Transcriptional profiling showed that Congo red treatment resulted in notable up-regulation of the core genes involving in cell wall integrity pathway in ram1Δ sporidia compared with that of WT, indicating that Ram1 may be involved in cell wall integrity regulation. In yeast the heterodimeric FTase is responsible for post-translational modification of Ras (small G protein) and a-factor (pheromone). We also identified and characterized two conserved Ras proteins, Ras1 and Ras2, respectively, and a MAT-1 pheromone precursor Mfa1. The ras1Δ, ras2Δ and mfa1Δ mutants all displayed reduced mating/filamentation similar as the ram1Δ mutant. However, both ras1Δ and ras2Δ mutants were hypersensitive to Congo red while the mfa1Δ mutant was the same as wild-type. Overall our study displayed that RAM1 plays an essential role in S. scitamineum mating/filamentation, pathogenicity, and cell wall stability. The basidiomycetous fungus causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for infection to the host; however, its underlying molecular mechanism has not been fully studied. In this study, we identified a conserved farnesyltransferase (FTase) β subunit Ram1 in . The Δ mutant displayed significantly reduced mating/filamentation, thus of weak pathogenicity to the host cane. The Δ mutant sporidia showed more tolerant toward cell wall stressor Congo red compared to that of the wild-type. Transcriptional profiling showed that Congo red treatment resulted in notable up-regulation of the core genes involving in cell wall integrity pathway in Δ sporidia compared with that of WT, indicating that Ram1 may be involved in cell wall integrity regulation. In yeast the heterodimeric FTase is responsible for post-translational modification of Ras (small G protein) and a-factor (pheromone). We also identified and characterized two conserved Ras proteins, Ras1 and Ras2, respectively, and a pheromone precursor Mfa1. The Δ, Δ and Δ mutants all displayed reduced mating/filamentation similar as the Δ mutant. However, both Δ and Δ mutants were hypersensitive to Congo red while the Δ mutant was the same as wild-type. Overall our study displayed that plays an essential role in mating/filamentation, pathogenicity, and cell wall stability. The basidiomycetous fungus Sporisorium scitamineum causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for infection to the host; however, its underlying molecular mechanism has not been fully studied. In this study, we identified a conserved farnesyltransferase (FTase) β subunit Ram1 in S. scitamineum . The ram1 Δ mutant displayed significantly reduced mating/filamentation, thus of weak pathogenicity to the host cane. The ram1 Δ mutant sporidia showed more tolerant toward cell wall stressor Congo red compared to that of the wild-type. Transcriptional profiling showed that Congo red treatment resulted in notable up-regulation of the core genes involving in cell wall integrity pathway in ram1 Δ sporidia compared with that of WT, indicating that Ram1 may be involved in cell wall integrity regulation. In yeast the heterodimeric FTase is responsible for post-translational modification of Ras (small G protein) and a-factor (pheromone). We also identified and characterized two conserved Ras proteins, Ras1 and Ras2, respectively, and a MAT-1 pheromone precursor Mfa1. The ras1 Δ, ras2 Δ and mfa1 Δ mutants all displayed reduced mating/filamentation similar as the ram1 Δ mutant. However, both ras1 Δ and ras2 Δ mutants were hypersensitive to Congo red while the mfa1 Δ mutant was the same as wild-type. Overall our study displayed that RAM1 plays an essential role in S. scitamineum mating/filamentation, pathogenicity, and cell wall stability. The basidiomycetous fungus Sporisorium scitamineum causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for infection to the host; however, its underlying molecular mechanism has not been fully studied. In this study, we identified a conserved farnesyltransferase (FTase) β subunit Ram1 in S. scitamineum. The ram1Δ mutant displayed significantly reduced mating/filamentation, thus of weak pathogenicity to the host cane. The ram1Δ mutant sporidia showed more tolerant toward cell wall stressor Congo red compared to that of the wild-type. Transcriptional profiling showed that Congo red treatment resulted in notable up-regulation of the core genes involving in cell wall integrity pathway in ram1Δ sporidia compared with that of WT, indicating that Ram1 may be involved in cell wall integrity regulation. In yeast the heterodimeric FTase is responsible for post-translational modification of Ras (small G protein) and a-factor (pheromone). We also identified and characterized two conserved Ras proteins, Ras1 and Ras2, respectively, and a MAT-1 pheromone precursor Mfa1. The ras1Δ, ras2Δ and mfa1Δ mutants all displayed reduced mating/filamentation similar as the ram1Δ mutant. However, both ras1Δ and ras2Δ mutants were hypersensitive to Congo red while the mfa1Δ mutant was the same as wild-type. Overall our study displayed that RAM1 plays an essential role in S. scitamineum mating/filamentation, pathogenicity, and cell wall stability. |
| Author | Jiang, Zide Cai, Enping Li, Lingyu Yan, Meixin Sun, Shuquan Deng, Yizhen Chen, Baoshan Chang, Changqing |
| AuthorAffiliation | 1 Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University , Guangzhou , China 2 Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences , Nanning , China 3 State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University , Nanning , China |
| AuthorAffiliation_xml | – name: 2 Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences , Nanning , China – name: 3 State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University , Nanning , China – name: 1 Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University , Guangzhou , China |
| Author_xml | – sequence: 1 givenname: Shuquan surname: Sun fullname: Sun, Shuquan – sequence: 2 givenname: Yizhen surname: Deng fullname: Deng, Yizhen – sequence: 3 givenname: Enping surname: Cai fullname: Cai, Enping – sequence: 4 givenname: Meixin surname: Yan fullname: Yan, Meixin – sequence: 5 givenname: Lingyu surname: Li fullname: Li, Lingyu – sequence: 6 givenname: Baoshan surname: Chen fullname: Chen, Baoshan – sequence: 7 givenname: Changqing surname: Chang fullname: Chang, Changqing – sequence: 8 givenname: Zide surname: Jiang fullname: Jiang, Zide |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31134021$$D View this record in MEDLINE/PubMed |
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| Copyright | Copyright © 2019 Sun, Deng, Cai, Yan, Li, Chen, Chang and Jiang. 2019 Sun, Deng, Cai, Yan, Li, Chen, Chang and Jiang |
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| Keywords | cell wall integrity Sporisorium scitamineum RAM1 Ras mating |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Fungi and Their Interactions, a section of the journal Frontiers in Microbiology Reviewed by: Andrei Steindorff, Joint Genome Institute and Lawrence Berkeley National Laboratory, United States; Jarrod R. Fortwendel, The University of Tennessee Health Science Center, United States Edited by: Fausto Almeida, University of São Paulo, Brazil |
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causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for infection to the host;... The basidiomycetous fungus Sporisorium scitamineum causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for... The basidiomycetous fungus Sporisorium scitamineum causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for... |
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| Title | The Farnesyltransferase β-Subunit Ram1 Regulates Sporisorium scitamineum Mating, Pathogenicity and Cell Wall Integrity |
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