Absence of epstein–barr virus DNA in the tumor cells of european hepatocellular carcinoma
The Epstein–Barr virus (EBV) has recently been associated with hepatocellular carcinoma (HCC) arising in Japanese patients. We analyzed 82 cases of HCC from Germany and the U.K. for the presence of EBV DNA and viral gene products within tumor cells. Initial screening of whole sections using quantita...
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Published in | Virology (New York, N.Y.) Vol. 306; no. 2; pp. 236 - 243 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
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Elsevier Inc
15.02.2003
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Abstract | The Epstein–Barr virus (EBV) has recently been associated with hepatocellular carcinoma (HCC) arising in Japanese patients. We analyzed 82 cases of HCC from Germany and the U.K. for the presence of EBV DNA and viral gene products within tumor cells. Initial screening of whole sections using quantitative (Q)-PCR detected EBV DNA in 9/58 U.K. cases and in 9/24 German cases; in positive cases viral load was very low, ranging between 1.4 and 49.1 copies of the EBV genome/1000 cell equivalents, compared to much higher values for EBV-positive Hodgkin’s disease and nasopharyngeal carcinoma controls (range, 714–3259/1000 cells). EBV DNA was not detected in the tumor cells of any of the Q-PCR-positive cases either by Q-PCR of pure tumor cell populations isolated by laser capture microdissection or by isotopic in situ hybridization. Furthermore, none of the German or U.K. HCC tumors tested positive for EBER or EBNAI expression in tumor cells. Our results provide strong evidence that HCCs from the U.K. or Germany are not associated with EBV. |
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AbstractList | The Epstein-Barr virus (EBV) has recently been associated with hepatocellular carcinoma (HCC) arising in Japanese patients. We analyzed 82 cases of HCC from Germany and the U.K. for the presence of EBV DNA and viral gene products within tumor cells. Initial screening of whole sections using quantitative (Q)-PCR detected EBV DNA in 9/58 U.K. cases and in 9/24 German cases; in positive cases viral load was very low, ranging between 1.4 and 49.1 copies of the EBV genome/1000 cell equivalents, compared to much higher values for EBV-positive Hodgkin's disease and nasopharyngeal carcinoma controls (range, 714-3259/1000 cells). EBV DNA was not detected in the tumor cells of any of the Q-PCR-positive cases either by Q-PCR of pure tumor cell populations isolated by laser capture microdissection or by isotopic in situ hybridization. Furthermore, none of the German or U.K. HCC tumors tested positive for EBER or EBNAI expression in tumor cells. Our results provide strong evidence that HCCs from the U.K. or Germany are not associated with EBV. |
Author | Murray, Paul G Bell, Andrew Young, Lawrence S Davies, Gillian Herrmann, Kathrin Timms, Judith Niedobitek, Gerald Junying, Jia Reynolds, Gary M Lissauer, David Hubscher, Stefan G |
Author_xml | – sequence: 1 givenname: Jia surname: Junying fullname: Junying, Jia organization: Department of Pathology, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK – sequence: 2 givenname: Kathrin surname: Herrmann fullname: Herrmann, Kathrin organization: Pathological Institute, Friedrich-Alexander-University, 91054 Erlangen, Germany – sequence: 3 givenname: Gillian surname: Davies fullname: Davies, Gillian organization: Department of Pathology, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK – sequence: 4 givenname: David surname: Lissauer fullname: Lissauer, David organization: Department of Pathology, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK – sequence: 5 givenname: Andrew surname: Bell fullname: Bell, Andrew organization: Cancer Research UK Institute for Cancer Studies, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK – sequence: 6 givenname: Judith surname: Timms fullname: Timms, Judith organization: Cancer Research UK Institute for Cancer Studies, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK – sequence: 7 givenname: Gary M surname: Reynolds fullname: Reynolds, Gary M organization: Liver Research Laboratories, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK – sequence: 8 givenname: Stefan G surname: Hubscher fullname: Hubscher, Stefan G organization: Department of Pathology, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK – sequence: 9 givenname: Lawrence S surname: Young fullname: Young, Lawrence S organization: Cancer Research UK Institute for Cancer Studies, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK – sequence: 10 givenname: Gerald surname: Niedobitek fullname: Niedobitek, Gerald organization: Pathological Institute, Friedrich-Alexander-University, 91054 Erlangen, Germany – sequence: 11 givenname: Paul G surname: Murray fullname: Murray, Paul G email: p.g.murray@bham.ac.uk organization: Department of Pathology, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK |
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Cites_doi | 10.1002/(SICI)1096-9896(199601)178:1<44::AID-PATH471>3.0.CO;2-0 10.1099/0022-1317-72-12-3035 10.1002/(SICI)1097-0215(19971114)73:4<514::AID-IJC10>3.0.CO;2-9 10.1093/jnci/92.8.655 10.1080/003655200750023075 10.1200/JCO.2001.19.2.420 10.1093/ajcp/103.3.308 10.1006/viro.1999.9619 10.1016/0092-8674(88)90530-2 10.1016/S0092-1157(85)80024-X 10.1182/blood.V84.11.3792.bloodjournal84113792 10.1002/(SICI)1097-0215(19990820)84:4<442::AID-IJC20>3.0.CO;2-J 10.1101/gr.6.10.986 10.1016/S0002-9440(10)62515-1 |
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Keywords | Hepatocellular carcinoma Laser capture microdissection In situ hybridization Epstein–Barr virus Quantitative PCR |
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References | Lawrence, Villnave, Singer (BIB8) 1988; 52 Chu, Chen, Chen, Weiss (BIB2) 2001; 159 Niedobitek, Mutimer, Williams, Whitehead, Wilson, Rooney, Young, Hubscher (BIB11) 1997; 73 Sugawara, Mizugaki, Uchida, Torii, Imai, Makuuchi, Takada (BIB13) 1999; 256 Brink, van Den Brule, van Diest, Meijer (BIB1) 2000; 92 Heid, Stevens, Livak, Williams (BIB5) 1996; 6 Whitaker (BIB16) 1985; 13 Grasser, Murray, Kremmer, Klein, Remberger, Feiden, Reynolds, Niedobitek, Young, Mueller-Lantzsch (BIB4) 1994; 84 Shibata, Weiss (BIB12) 1992; 140 Niedobitek, Young, Lau, Brooks, Greenspan, Greenspan, Rickinson (BIB10) 1991; 72 Gallagher, Armstrong, MacKenzie, Shield, Khan, Lake, Proctor, Taylor, Clements, Jarrett (BIB3) 1999; 84 Klein (BIB7) 1979 Murray, Niedobitek, Kremmer, Grasser, Reynolds, Cruchley, Williams, Muller-Lantzsch, Young (BIB9) 1996; 178 Sugawara, Makuuchi, Takada (BIB14) 2000; 35 Summers, Goff, Wilson, Gupta, Lister, Fitzgibbon (BIB15) 2001; 19 Iezzoni, Gaffey, Weiss (BIB6) 1995; 103 Niedobitek (10.1016/S0042-6822(02)00027-2_BIB10) 1991; 72 Lawrence (10.1016/S0042-6822(02)00027-2_BIB8) 1988; 52 Summers (10.1016/S0042-6822(02)00027-2_BIB15) 2001; 19 Grasser (10.1016/S0042-6822(02)00027-2_BIB4) 1994; 84 Iezzoni (10.1016/S0042-6822(02)00027-2_BIB6) 1995; 103 Murray (10.1016/S0042-6822(02)00027-2_BIB9) 1996; 178 Sugawara (10.1016/S0042-6822(02)00027-2_BIB14) 2000; 35 Gallagher (10.1016/S0042-6822(02)00027-2_BIB3) 1999; 84 Brink (10.1016/S0042-6822(02)00027-2_BIB1) 2000; 92 Sugawara (10.1016/S0042-6822(02)00027-2_BIB13) 1999; 256 Whitaker (10.1016/S0042-6822(02)00027-2_BIB16) 1985; 13 Klein (10.1016/S0042-6822(02)00027-2_BIB7) 1979 Chu (10.1016/S0042-6822(02)00027-2_BIB2) 2001; 159 Shibata (10.1016/S0042-6822(02)00027-2_BIB12) 1992; 140 Heid (10.1016/S0042-6822(02)00027-2_BIB5) 1996; 6 Niedobitek (10.1016/S0042-6822(02)00027-2_BIB11) 1997; 73 |
References_xml | – volume: 84 start-page: 442 year: 1999 end-page: 448 ident: BIB3 article-title: Detection of Epstein–Barr virus (EBV) genomes in the serum of patients with publication-title: Int. J. Cancer contributor: fullname: Jarrett – volume: 13 start-page: 173 year: 1985 end-page: 175 ident: BIB16 article-title: The chromosomes of the Namalwa cell line publication-title: J. Biol. Stand. contributor: fullname: Whitaker – volume: 6 start-page: 986 year: 1996 end-page: 994 ident: BIB5 article-title: Real time quantitative PCR publication-title: Genome Res. contributor: fullname: Williams – volume: 52 start-page: 51 year: 1988 end-page: 61 ident: BIB8 article-title: Sensitive, high-resolution chromatin and chromosome mapping in situ publication-title: Cell contributor: fullname: Singer – volume: 19 start-page: 420 year: 2001 end-page: 424 ident: BIB15 article-title: Implications for the monitoring of disease in patients with follicular lymphoma publication-title: J. Clin. Oncol. contributor: fullname: Fitzgibbon – volume: 35 start-page: 981 year: 2000 end-page: 984 ident: BIB14 article-title: Detection of Epstein-Barr virus DNA in hepatocellular carcinoma tissues from hepatitis C-positive patients publication-title: Scand. J. Gastroenterol. contributor: fullname: Takada – volume: 84 start-page: 3792 year: 1994 end-page: 3798 ident: BIB4 article-title: Monoclonal antibodies directed against the Epstein-Barr virus-encoded nuclear antigen 1 (EBNA1) publication-title: Blood contributor: fullname: Mueller-Lantzsch – volume: 178 start-page: 44 year: 1996 end-page: 47 ident: BIB9 article-title: In situ detection of the Epstein-Barr virus-encoded nuclear antigen 1 in oral hairy leukoplakia and virus-associated carcinomas publication-title: J. Pathol. contributor: fullname: Young – volume: 73 start-page: 514 year: 1997 end-page: 520 ident: BIB11 article-title: Epstein-Barr virus infection and malignant lymphomas in liver transplant recipients publication-title: Int. J. Cancer contributor: fullname: Hubscher – start-page: 339 year: 1979 end-page: 350 ident: BIB7 article-title: The relationship of the virus to nasopharyngeal carcinoma publication-title: The Epstein–Barr Virus contributor: fullname: Klein – volume: 72 start-page: 3035 year: 1991 end-page: 3046 ident: BIB10 article-title: Epstein- Barr virus infection in oral hairy leukoplakia publication-title: J. Gen. Virol. contributor: fullname: Rickinson – volume: 256 start-page: 196 year: 1999 end-page: 202 ident: BIB13 article-title: Detection of Epstein-Barr virus ( publication-title: Virology contributor: fullname: Takada – volume: 103 start-page: 308 year: 1995 end-page: 315 ident: BIB6 article-title: The role of Epstein–Barr virus in lymphoepithelioma-like carcinomas publication-title: Am. J. Clin. Pathol. contributor: fullname: Weiss – volume: 92 start-page: 655 year: 2000 end-page: 656 ident: BIB1 article-title: Detection of Epstein–Barr virus in invasive breast cancers publication-title: J. Natl. Cancer Inst. contributor: fullname: Meijer – volume: 159 start-page: 1287 year: 2001 end-page: 1292 ident: BIB2 article-title: No direct role for Epstein–Barr virus in American hepatocellular carcinoma publication-title: Am. J. Pathol. contributor: fullname: Weiss – volume: 140 start-page: 769 year: 1992 end-page: 841 ident: BIB12 article-title: Epstein-Barr virus associated gastric adenocarcinoma publication-title: Am. J. Pathol. contributor: fullname: Weiss – volume: 178 start-page: 44 year: 1996 ident: 10.1016/S0042-6822(02)00027-2_BIB9 article-title: In situ detection of the Epstein-Barr virus-encoded nuclear antigen 1 in oral hairy leukoplakia and virus-associated carcinomas publication-title: J. Pathol. doi: 10.1002/(SICI)1096-9896(199601)178:1<44::AID-PATH471>3.0.CO;2-0 contributor: fullname: Murray – volume: 72 start-page: 3035 year: 1991 ident: 10.1016/S0042-6822(02)00027-2_BIB10 article-title: Epstein- Barr virus infection in oral hairy leukoplakia publication-title: J. Gen. Virol. doi: 10.1099/0022-1317-72-12-3035 contributor: fullname: Niedobitek – volume: 73 start-page: 514 year: 1997 ident: 10.1016/S0042-6822(02)00027-2_BIB11 article-title: Epstein-Barr virus infection and malignant lymphomas in liver transplant recipients publication-title: Int. J. Cancer doi: 10.1002/(SICI)1097-0215(19971114)73:4<514::AID-IJC10>3.0.CO;2-9 contributor: fullname: Niedobitek – volume: 92 start-page: 655 year: 2000 ident: 10.1016/S0042-6822(02)00027-2_BIB1 article-title: Detection of Epstein–Barr virus in invasive breast cancers publication-title: J. Natl. Cancer Inst. doi: 10.1093/jnci/92.8.655 contributor: fullname: Brink – volume: 35 start-page: 981 year: 2000 ident: 10.1016/S0042-6822(02)00027-2_BIB14 article-title: Detection of Epstein-Barr virus DNA in hepatocellular carcinoma tissues from hepatitis C-positive patients publication-title: Scand. J. Gastroenterol. doi: 10.1080/003655200750023075 contributor: fullname: Sugawara – volume: 19 start-page: 420 year: 2001 ident: 10.1016/S0042-6822(02)00027-2_BIB15 article-title: Implications for the monitoring of disease in patients with follicular lymphoma publication-title: J. Clin. Oncol. doi: 10.1200/JCO.2001.19.2.420 contributor: fullname: Summers – volume: 103 start-page: 308 year: 1995 ident: 10.1016/S0042-6822(02)00027-2_BIB6 article-title: The role of Epstein–Barr virus in lymphoepithelioma-like carcinomas publication-title: Am. J. Clin. Pathol. doi: 10.1093/ajcp/103.3.308 contributor: fullname: Iezzoni – start-page: 339 year: 1979 ident: 10.1016/S0042-6822(02)00027-2_BIB7 article-title: The relationship of the virus to nasopharyngeal carcinoma contributor: fullname: Klein – volume: 256 start-page: 196 year: 1999 ident: 10.1016/S0042-6822(02)00027-2_BIB13 article-title: Detection of Epstein-Barr virus (EBV) in hepatocellular carcinoma tissue publication-title: Virology doi: 10.1006/viro.1999.9619 contributor: fullname: Sugawara – volume: 52 start-page: 51 year: 1988 ident: 10.1016/S0042-6822(02)00027-2_BIB8 article-title: Sensitive, high-resolution chromatin and chromosome mapping in situ publication-title: Cell doi: 10.1016/0092-8674(88)90530-2 contributor: fullname: Lawrence – volume: 13 start-page: 173 year: 1985 ident: 10.1016/S0042-6822(02)00027-2_BIB16 article-title: The chromosomes of the Namalwa cell line publication-title: J. Biol. Stand. doi: 10.1016/S0092-1157(85)80024-X contributor: fullname: Whitaker – volume: 140 start-page: 769 year: 1992 ident: 10.1016/S0042-6822(02)00027-2_BIB12 article-title: Epstein-Barr virus associated gastric adenocarcinoma publication-title: Am. J. Pathol. contributor: fullname: Shibata – volume: 84 start-page: 3792 year: 1994 ident: 10.1016/S0042-6822(02)00027-2_BIB4 article-title: Monoclonal antibodies directed against the Epstein-Barr virus-encoded nuclear antigen 1 (EBNA1) publication-title: Blood doi: 10.1182/blood.V84.11.3792.bloodjournal84113792 contributor: fullname: Grasser – volume: 84 start-page: 442 year: 1999 ident: 10.1016/S0042-6822(02)00027-2_BIB3 article-title: Detection of Epstein–Barr virus (EBV) genomes in the serum of patients with EBV-associated Hodgkin’s disease publication-title: Int. J. Cancer doi: 10.1002/(SICI)1097-0215(19990820)84:4<442::AID-IJC20>3.0.CO;2-J contributor: fullname: Gallagher – volume: 6 start-page: 986 year: 1996 ident: 10.1016/S0042-6822(02)00027-2_BIB5 article-title: Real time quantitative PCR publication-title: Genome Res. doi: 10.1101/gr.6.10.986 contributor: fullname: Heid – volume: 159 start-page: 1287 issue: 4 year: 2001 ident: 10.1016/S0042-6822(02)00027-2_BIB2 article-title: No direct role for Epstein–Barr virus in American hepatocellular carcinoma publication-title: Am. J. Pathol. doi: 10.1016/S0002-9440(10)62515-1 contributor: fullname: Chu |
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Snippet | The Epstein–Barr virus (EBV) has recently been associated with hepatocellular carcinoma (HCC) arising in Japanese patients. We analyzed 82 cases of HCC from... The Epstein-Barr virus (EBV) has recently been associated with hepatocellular carcinoma (HCC) arising in Japanese patients. We analyzed 82 cases of HCC from... |
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SubjectTerms | Base Sequence Carcinoma, Hepatocellular - virology DNA, Viral - genetics DNA, Viral - isolation & purification Epstein-Barr Virus Nuclear Antigens - genetics Epstein-Barr Virus Nuclear Antigens - metabolism Epstein–Barr virus Europe Genome, Viral Hepatocellular carcinoma Herpesvirus 4, Human - genetics Herpesvirus 4, Human - isolation & purification Humans Immunohistochemistry In Situ Hybridization Laser capture microdissection Liver Neoplasms - virology Lymphocytes - virology Polymerase Chain Reaction Quantitative PCR Ribosomal Proteins RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism |
Title | Absence of epstein–barr virus DNA in the tumor cells of european hepatocellular carcinoma |
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