Absence of epstein–barr virus DNA in the tumor cells of european hepatocellular carcinoma

• Published in: Virology (New York, N.Y.) Vol. 306; no. 2; pp. 236 - 243
• Main Authors: Junying, Jia, Herrmann, Kathrin, Davies, Gillian, Lissauer, David, Bell, Andrew, Timms, Judith, Reynolds, Gary M, Hubscher, Stefan G, Young, Lawrence S, Niedobitek, Gerald, Murray, Paul G
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.02.2003
• Subjects: Base Sequence; Carcinoma, Hepatocellular - virology; DNA, Viral - genetics; DNA, Viral - isolation & purification; Epstein-Barr Virus Nuclear Antigens - genetics; Epstein-Barr Virus Nuclear Antigens - metabolism; Epstein–Barr virus; Europe; Genome, Viral; Hepatocellular carcinoma; Herpesvirus 4, Human - genetics; Herpesvirus 4, Human - isolation & purification; Humans; Immunohistochemistry; In Situ Hybridization; Laser capture microdissection; Liver Neoplasms - virology; Lymphocytes - virology; Polymerase Chain Reaction; Quantitative PCR; Ribosomal Proteins; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Hepatocellular carcinoma; Laser capture microdissection; In situ hybridization; Epstein–Barr virus; Quantitative PCR
• DOI: 10.1016/S0042-6822(02)00027-2
• ISSN: 0042-6822

The Epstein–Barr virus (EBV) has recently been associated with hepatocellular carcinoma (HCC) arising in Japanese patients. We analyzed 82 cases of HCC from Germany and the U.K. for the presence of EBV DNA and viral gene products within tumor cells. Initial screening of whole sections using quantitative (Q)-PCR detected EBV DNA in 9/58 U.K. cases and in 9/24 German cases; in positive cases viral load was very low, ranging between 1.4 and 49.1 copies of the EBV genome/1000 cell equivalents, compared to much higher values for EBV-positive Hodgkin’s disease and nasopharyngeal carcinoma controls (range, 714–3259/1000 cells). EBV DNA was not detected in the tumor cells of any of the Q-PCR-positive cases either by Q-PCR of pure tumor cell populations isolated by laser capture microdissection or by isotopic in situ hybridization. Furthermore, none of the German or U.K. HCC tumors tested positive for EBER or EBNAI expression in tumor cells. Our results provide strong evidence that HCCs from the U.K. or Germany are not associated with EBV.