Improvement of Infusion Process in Cell Transplantation: Effect of Shear Stress on Hepatocyte Viability Under Horizontal and Vertical Syringe Orientation

Improving cell viability and function are important for enhancing the clinical results of cell transplantation. The relationship between cell viability and shear stress remains unexplained, and sedimentation effects during the infusion process are important to the hepatocyte transplantation process....

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Published inCell medicine Vol. 7; no. 2; pp. 59 - 66
Main Authors Sufiandi, Sandi, Obara, Hiromichi, Enosawa, Shin, Hsu, Huai-Che, Matsuno, Naoto, Mizunuma, Hiroshi
Format Journal Article
LanguageEnglish
Published United States Cognizant Communication Corporation 01.01.2015
Subjects
Shear stress
Syringe orientation
Hepatocytes
Viability loss
Rectangular microchannel
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Abstract Improving cell viability and function are important for enhancing the clinical results of cell transplantation. The relationship between cell viability and shear stress remains unexplained, and sedimentation effects during the infusion process are important to the hepatocyte transplantation process. In the present study, the relationship between cell viability and shear stress in the presence of sedimentation effect was investigated using a microchannel simulating the cell transplantation process under several shear stress conditions. Horizontal and vertical syringe orientations were employed to investigate the sedimentation effect. The vertical syringe orientation resulted in lower viability loss than the horizontal orientation. In summary, removing a sedimentation effect is important to improving cell viability by preventing high shear stress.
AbstractList Improving cell viability and function are important for enhancing the clinical results of cell transplantation. The relationship between cell viability and shear stress remains unexplained, and sedimentation effects during the infusion process are important to the hepatocyte transplantation process. In the present study, the relationship between cell viability and shear stress in the presence of sedimentation effect was investigated using a microchannel simulating the cell transplantation process under several shear stress conditions. Horizontal and vertical syringe orientations were employed to investigate the sedimentation effect. The vertical syringe orientation resulted in lower viability loss than the horizontal orientation. In summary, removing a sedimentation effect is important to improving cell viability by preventing high shear stress.
Author Sufiandi, Sandi
Mizunuma, Hiroshi
Obara, Hiromichi
Hsu, Huai-Che
Enosawa, Shin
Matsuno, Naoto
AuthorAffiliation Department of Mechanical Engineering, Graduate School of Science and Engineering, Tokyo Metropolitan University , Hachioji, Tokyo , Japan
Clinical Research Center, National Center for Child Health and Development , Setagaya-ku, Tokyo , Japan
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Cites_doi 10.1542/peds.111.6.1262
10.1016/j.transproceed.2008.03.043
10.1007/s001090100260
10.1016/j.transproceed.2008.03.110
10.1016/S0041-1345(97)00252-2
10.1097/TP.0b013e318199936a
10.3727/000000007783465019
10.1053/j.gastro.2006.01.008
10.1002/lt.21443
10.1016/j.ijmultiphaseflow.2004.12.004
10.1097/00007890-199702270-00014
10.3748/wjg.14.3464
10.1056/NEJM199805143382004
10.1002/0471142735.ima03bs21
10.1097/01.TP.0000113809.53415.C2
10.1097/TP.0b013e31823b72d6
10.1002/lt.23800
10.1097/TP.0b013e3181963ad3
10.1111/j.1365-2796.2009.02152.x
10.3727/096368911X566208
10.1097/01.TP.0000077420.81365.53
10.1055/s-2007-1007096
10.1111/j.1432-2277.2005.00127.x
10.1038/ng0494-335
10.1038/nrgastro.2010.44
10.1097/00000658-199602000-00002
10.1016/j.jhep.2010.05.010
10.1016/j.transproceed.2008.03.022
10.1097/01.tp.0000231689.44266.ac
10.3727/096368909X470775
10.1111/j.1600-6143.2005.00935.x
10.1111/j.1432-1033.1981.tb05560.x
10.1097/01.tp.0000232451.93703.ab
10.1177/014107680509800803
10.1111/j.1365-2796.2012.02574.x
10.3727/000000006783982395
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Syringe orientation
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Viability loss
Rectangular microchannel
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Received May 21, 2014; final acceptance November 18, 2014. Online prepub date: December 12, 2014.
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References 11715065 - J Mol Med (Berl). 2001 Nov;79(11):617-30
12777539 - Pediatrics. 2003 Jun;111(6 Pt 1):1262-7
8054972 - Nat Genet. 1994 Apr;6(4):335-41
18555137 - Transplant Proc. 2008 May;40(4):1153-5
16826802 - Cell Transplant. 2006;15 Suppl 1:S105-10
12973120 - Transplantation. 2003 Aug 27;76(4):735-8
17060866 - Transplantation. 2006 Oct 27;82(8):1115-6
20667616 - J Hepatol. 2010 Oct;53(4):769-74
19307780 - Transplantation. 2009 Feb 27;87(4):457-66
22789058 - J Intern Med. 2012 Sep;272(3):201-23
15996260 - Am J Transplant. 2005 Aug;5(8):2058-61
19765179 - J Intern Med. 2009 Oct;266(4):339-57
9193547 - Transplant Proc. 1997 Jun;29(4):2103-6
16618422 - Gastroenterology. 2006 Apr;130(4):1317-23
18567072 - World J Gastroenterol. 2008 Jun 14;14(22):3464-70
10349682 - Semin Liver Dis. 1999;19(1):39-48
15910305 - Transpl Int. 2005 Jun;18(6):750-4
24273015 - Liver Transpl. 2014 Mar;20(3):391-3
18555136 - Transplant Proc. 2008 May;40(4):1148-50
21457616 - Cell Transplant. 2012;21(1):1-10
19295306 - Transplantation. 2009 Mar 15;87(5):636-41
177845 - Methods Cell Biol. 1976;13:29-83
18433045 - Liver Transpl. 2008 May;14(5):688-94
8597504 - Ann Surg. 1996 Feb;223(2):116-26
20368738 - Nat Rev Gastroenterol Hepatol. 2010 May;7(5):288-98
9047152 - Transplantation. 1997 Feb 27;63(4):559-69
16055896 - J R Soc Med. 2005 Aug;98(8):341-5
9580649 - N Engl J Med. 1998 May 14;338(20):1422-6
15239608 - Transplantation. 2004 May 27;77(10):1481-6
7028482 - Eur J Biochem. 1981 Sep 1;118(3):591-7
17912954 - Cell Transplant. 2007;16(6):629-37
18555083 - Transplant Proc. 2008 May;40(4):936-7
16926585 - Transplantation. 2006 Aug 27;82(4):441-9
19796496 - Cell Transplant. 2009;18(7):777-86
22082820 - Transplantation. 2012 Feb 27;93(4):342-7
bibr18-215517914X685150
bibr30-215517914X685150
bibr35-215517914X685150
bibr9-215517914X685150
bibr21-215517914X685150
bibr5-215517914X685150
bibr14-215517914X685150
bibr12-215517914X685150
bibr31-215517914X685150
bibr25-215517914X685150
bibr17-215517914X685150
bibr34-215517914X685150
bibr4-215517914X685150
bibr22-215517914X685150
bibr8-215517914X685150
bibr26-215517914X685150
bibr13-215517914X685150
bibr24-215517914X685150
Seglen P. O. (bibr27-215517914X685150) 1976
bibr37-215517914X685150
bibr2-215517914X685150
bibr32-215517914X685150
bibr3-215517914X685150
bibr38-215517914X685150
bibr16-215517914X685150
bibr10-215517914X685150
bibr29-215517914X685150
bibr7-215517914X685150
bibr1-215517914X685150
bibr36-215517914X685150
bibr23-215517914X685150
bibr20-215517914X685150
bibr19-215517914X685150
bibr33-215517914X685150
bibr15-215517914X685150
bibr6-215517914X685150
bibr11-215517914X685150
bibr28-215517914X685150
References_xml – ident: bibr12-215517914X685150
  doi: 10.1542/peds.111.6.1262
– ident: bibr22-215517914X685150
  doi: 10.1016/j.transproceed.2008.03.043
– ident: bibr23-215517914X685150
  doi: 10.1007/s001090100260
– ident: bibr16-215517914X685150
  doi: 10.1016/j.transproceed.2008.03.110
– ident: bibr37-215517914X685150
  doi: 10.1016/S0041-1345(97)00252-2
– ident: bibr21-215517914X685150
  doi: 10.1097/TP.0b013e318199936a
– ident: bibr18-215517914X685150
  doi: 10.3727/000000007783465019
– ident: bibr32-215517914X685150
  doi: 10.1053/j.gastro.2006.01.008
– ident: bibr1-215517914X685150
  doi: 10.1002/lt.21443
– ident: bibr31-215517914X685150
  doi: 10.1016/j.ijmultiphaseflow.2004.12.004
– ident: bibr38-215517914X685150
  doi: 10.1097/00007890-199702270-00014
– ident: bibr19-215517914X685150
  doi: 10.3748/wjg.14.3464
– ident: bibr8-215517914X685150
  doi: 10.1056/NEJM199805143382004
– ident: bibr34-215517914X685150
  doi: 10.1002/0471142735.ima03bs21
– ident: bibr11-215517914X685150
  doi: 10.1097/01.TP.0000113809.53415.C2
– start-page: 29
  volume-title: Methods in Cell Biology.
  year: 1976
  ident: bibr27-215517914X685150
  contributor:
    fullname: Seglen P. O.
– ident: bibr13-215517914X685150
  doi: 10.1097/TP.0b013e31823b72d6
– ident: bibr5-215517914X685150
  doi: 10.1002/lt.23800
– ident: bibr28-215517914X685150
  doi: 10.1097/TP.0b013e3181963ad3
– ident: bibr7-215517914X685150
  doi: 10.1111/j.1365-2796.2009.02152.x
– ident: bibr24-215517914X685150
  doi: 10.3727/096368911X566208
– ident: bibr29-215517914X685150
  doi: 10.1097/01.TP.0000077420.81365.53
– ident: bibr36-215517914X685150
  doi: 10.1055/s-2007-1007096
– ident: bibr2-215517914X685150
  doi: 10.1111/j.1432-2277.2005.00127.x
– ident: bibr9-215517914X685150
  doi: 10.1038/ng0494-335
– ident: bibr10-215517914X685150
– ident: bibr4-215517914X685150
  doi: 10.1038/nrgastro.2010.44
– ident: bibr25-215517914X685150
  doi: 10.1097/00000658-199602000-00002
– ident: bibr30-215517914X685150
  doi: 10.1016/j.jhep.2010.05.010
– ident: bibr17-215517914X685150
  doi: 10.1016/j.transproceed.2008.03.022
– ident: bibr6-215517914X685150
  doi: 10.1097/01.tp.0000231689.44266.ac
– ident: bibr20-215517914X685150
  doi: 10.3727/096368909X470775
– ident: bibr33-215517914X685150
  doi: 10.1111/j.1600-6143.2005.00935.x
– ident: bibr3-215517914X685150
  doi: 10.1111/j.1432-1033.1981.tb05560.x
– ident: bibr26-215517914X685150
  doi: 10.1097/01.tp.0000232451.93703.ab
– ident: bibr14-215517914X685150
  doi: 10.1177/014107680509800803
– ident: bibr15-215517914X685150
  doi: 10.1111/j.1365-2796.2012.02574.x
– ident: bibr35-215517914X685150
  doi: 10.3727/000000006783982395
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Title Improvement of Infusion Process in Cell Transplantation: Effect of Shear Stress on Hepatocyte Viability Under Horizontal and Vertical Syringe Orientation
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