Optimization of extracellular glucan production from Pleurotus eryngii and its impact on angiogenesis

• Published in: International journal of biological macromolecules Vol. 50; no. 4; pp. 957 - 964
• Main Authors: Shenbhagaraman, Ramalingam, Jagadish, Loganathan K., Premalatha, Kishorekumar, Kaviyarasan, Venkatesan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2012
• Subjects: angiogenesis; Angiogenesis Inhibitors - biosynthesis; Angiogenesis Inhibitors - pharmacology; Angiogenesis Inhibitors - therapeutic use; Anti-angiogenesis; Biomass; Box–Behnken; CAM assay; Chorioallantoic Membrane - blood supply; Chorioallantoic Membrane - drug effects; Extracellular polysaccharide; Extracellular Space - metabolism; Fermentation; glucans; Glucans - biosynthesis; Glucans - pharmacology; Glucans - therapeutic use; glucose; King oyster; Models, Statistical; mushrooms; mycelium; Mycelium - growth & development; Neovascularization, Pathologic - drug therapy; Pleurotus - cytology; Pleurotus - growth & development; Pleurotus - metabolism; Pleurotus eryngii; prediction; response surface methodology; temperature; yeast extract; Extracellular polysaccharide; King oyster; Anti-angiogenesis; Box–Behnken; CAM assay
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2012.02.008

► Pleurotus eryngii, an edible mushroom optimized using response surface methodology of four-factor Box–Behnken design. ► The optimum levels of glucose, yeast extract, pH and temperature were determined for maximal yield of mycelial biomass and extra cellular polysaccharide. ► The Box Behnken design matrix was validated using real-time experiments. ► Extracellular polysaccharide of Pleurotus eryngii was found to be a glucan. ► Extracellular glucans significantly inhibited the neo-vascularization above 500μg/egg. Pleurotus eryngii, an edible mushroom with therapeutic potential was optimized using response surface methodology of four-factor Box–Behnken design for maximum mycelial biomass and extracellular glucan (EPS) production. The model predicts to gain a maximal mycelial biomass and extracellular polysaccharide at 39.4g/l; 36.04g/l of glucose, 8.27g/l; 7.51g/l of yeast extract, pH 6.99; 7.07 and temperature 26.2°C; 25.84°C, respectively. The validation experiments showed that the model was significant and in close agreement with the model prediction. The evaluation of extracellular polysaccharide on angiogenesis by ex vivo CAM assay showed that there was significant inhibition in neo-vascularization.