Optimization of extracellular glucan production from Pleurotus eryngii and its impact on angiogenesis
► Pleurotus eryngii, an edible mushroom optimized using response surface methodology of four-factor Box–Behnken design. ► The optimum levels of glucose, yeast extract, pH and temperature were determined for maximal yield of mycelial biomass and extra cellular polysaccharide. ► The Box Behnken design...
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Published in | International journal of biological macromolecules Vol. 50; no. 4; pp. 957 - 964 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.05.2012
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Subjects | |
Online Access | Get full text |
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Summary: | ► Pleurotus eryngii, an edible mushroom optimized using response surface methodology of four-factor Box–Behnken design. ► The optimum levels of glucose, yeast extract, pH and temperature were determined for maximal yield of mycelial biomass and extra cellular polysaccharide. ► The Box Behnken design matrix was validated using real-time experiments. ► Extracellular polysaccharide of Pleurotus eryngii was found to be a glucan. ► Extracellular glucans significantly inhibited the neo-vascularization above 500μg/egg.
Pleurotus eryngii, an edible mushroom with therapeutic potential was optimized using response surface methodology of four-factor Box–Behnken design for maximum mycelial biomass and extracellular glucan (EPS) production. The model predicts to gain a maximal mycelial biomass and extracellular polysaccharide at 39.4g/l; 36.04g/l of glucose, 8.27g/l; 7.51g/l of yeast extract, pH 6.99; 7.07 and temperature 26.2°C; 25.84°C, respectively. The validation experiments showed that the model was significant and in close agreement with the model prediction. The evaluation of extracellular polysaccharide on angiogenesis by ex vivo CAM assay showed that there was significant inhibition in neo-vascularization. |
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Bibliography: | http://dx.doi.org/10.1016/j.ijbiomac.2012.02.008 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0141-8130 1879-0003 |
DOI: | 10.1016/j.ijbiomac.2012.02.008 |