Characterization of zetin 1/rBSPRY, a novel binding partner of 14-3-3 proteins

• Published in: Biochemical and biophysical research communications Vol. 302; no. 3; pp. 526 - 533
• Main Authors: Birkenfeld, Jörg, Kartmann, Bettina, Anliker, Brigitte, Ono, Koji, Schlötcke, Britta, Betz, Heinrich, Roth, Dagmar
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.03.2003
• Subjects: 14-3-3 Proteins; Actin; Amino Acid Sequence; Animals; B-box; Blotting, Northern; Brain - metabolism; Brain expression; Cell Line; COS Cells; DNA, Complementary - metabolism; Gene Library; Humans; In Situ Hybridization; Molecular Sequence Data; Plasmids - metabolism; Precipitin Tests; Protein Binding; Protein Biosynthesis; Protein Isoforms; Proteins - chemistry; Proteins - metabolism; Rats; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; SPRY domain; Tissue Distribution; Transcription, Genetic; Transfection; Two-Hybrid System Techniques; Tyrosine 3-Monooxygenase - metabolism; B-box; SPRY domain; 14-3-3 Proteins; Brain expression; Actin
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(03)00182-7

14-3-3 proteins are ubiquitously expressed proteins which serve as central adaptors in different signal transduction cascades. In this study, yeast two-hybrid screening of a rat brain cDNA library identified a novel gene product termed zetin 1/rBSPRY that interacts with 14-3-3ζ. The zetin 1/rBSPRY gene is ubiquitously expressed in a variety of rat tissues, with highest expression being found in testis. In adult brain, high levels of zetin 1/rBSPRY mRNA were observed in the hippocampus, cereberal cortex, and piriform cortex. Biochemical studies confirmed zetin 1/rBSPRY to interact with 14-3-3ζ. Transient co-transfection in COS 7 cells caused a partial redistribution of zetin 1/rBSPRY into 14-3-3ζ enriched submembranous foci at leading edges. Our results suggest a role for zetin 1/rBSPRY-14-3-3 interactions at specialized submembrane domains.