Localization of the small GTP-binding protein rab1p to early compartments of the secretory pathway

• Published in: Journal of cell science Vol. 108 ( Pt 4); no. 4; pp. 1541 - 1552
• Main Authors: Saraste, J, Lahtinen, U, Goud, B
• Format: Journal Article
• Language: English
• Published: England 01.04.1995
• Subjects: Animals; Antibodies; Biomarkers - analysis; Cell Fractionation; Cell Line; Endoplasmic Reticulum - ultrastructure; Fluorescent Antibody Technique; Golgi Apparatus - metabolism; Golgi Apparatus - ultrastructure; GTP Phosphohydrolases - analysis; GTP-Binding Proteins - analysis; Immunoenzyme Techniques; Kidney; Mice; Microscopy, Immunoelectron; Microsomes - metabolism; Microsomes - ultrastructure; Multiple Myeloma; Organelles - metabolism; Organelles - ultrastructure; Pancreas - metabolism; Pancreas - ultrastructure; rab GTP-Binding Proteins; Rats; Saccharomyces cerevisiae Proteins

We have studied the localization of the small GTPase rab1p in different cell types using polyclonal antibodies prepared against the rab1A isoform of the protein. Immunofluorescence microscopy of normal rat kidney (NRK) and mouse myeloma cells showed the association of the protein with the Golgi comp...