Engineering Escherichia coli for efficient glutathione production

• Published in: Metabolic engineering Vol. 84; pp. 180 - 190
• Main Authors: Mori, Hiroki, Matsui, Misato, Bamba, Takahiro, Hori, Yoshimi, Kitamura, Sayaka, Toya, Yoshihiro, Hidese, Ryota, Yasueda, Hisashi, Hasunuma, Tomohisa, Shimizu, Hiroshi, Taoka, Naoaki, Kobayashi, Shingo
• Format: Journal Article
• Language: English
• Published: Belgium Elsevier Inc 01.07.2024
• Subjects: ammonium sulfate; batch fermentation; culture media; cysteine; Escherichia coli; glutamic acid; glutathione; Glutathione fermentation; glutathione synthase; Metabolic flux analysis (MFA); Metabolomic analysis; metabolomics; oxidoreductases; reaction kinetics; sodium thiosulfate; sulfur; tripeptides; yeasts; Glutathione fermentation; Metabolic flux analysis (MFA); Metabolomic analysis; Escherichia coli

Glutathione is a tripeptide of excellent value in the pharmaceutical, food, and cosmetic industries that is currently produced during yeast fermentation. In this case, glutathione accumulates intracellularly, which hinders high production. Here, we engineered Escherichia coli for the efficient produ...