Second harmonic imaging of membrane potential of neurons with retinal

• Published in: Journal of biomedical optics Vol. 9; no. 5; p. 873
• Main Authors: Nemet, Boaz A, Nikolenko, Volodymyr, Yuste, Rafael
• Format: Journal Article
• Language: English
• Published: United States 01.09.2004
• Subjects: Animals; Animals, Newborn; Cells, Cultured; Image Enhancement - methods; Kidney - cytology; Kidney - physiology; Membrane Potentials - physiology; Microscopy, Confocal - methods; Neurons - cytology; Neurons - physiology; Patch-Clamp Techniques - methods; Rats; Rats, Sprague-Dawley; Retinaldehyde; Staining and Labeling - methods

We present a method to optically measure and image the membrane potential of neurons, using the nonlinear optical phenomenon of second harmonic generation (SHG) with a photopigment retinal as the chromophore [second harmonic retinal imaging of membrane potential (SHRIMP)]. We show that all-trans ret...