HPLC electrochemical detection of trace amines in human plasma and platelets and expression of mRNA transcripts of trace amine receptors in circulating leukocytes

• Published in: Neuroscience letters Vol. 346; no. 1; pp. 89 - 92
• Main Authors: D'Andrea, Giovanni, Terrazzino, Salvatore, Fortin, Dino, Farruggio, Angelo, Rinaldi, Luciano, Leon, Alberta
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 31.07.2003; Elsevier
• Subjects: Adult; Biogenic Amines - blood; Biological and medical sciences; Blood Platelets - metabolism; Chromatography, High Pressure Liquid - methods; Electrochemistry; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - physiology; General aspects; High performance liquid chromatography electrochemical detection; Human plasma; Humans; Leukocytes - metabolism; Male; Octopamine; Platelets; RNA, Messenger - biosynthesis; RNA, Messenger - blood; Synephrine; Trace amine receptors; Tyramine; Tyramine - blood; Tyramine - metabolism; Vertebrates: cardiovascular system; Tyramine; Human plasma; Octopamine; Trace amine receptors; Platelets; Synephrine; High performance liquid chromatography electrochemical detection; Human; Leukocyte; HPLC chromatography; Gene expression; Oxedrine; Blood plasma; Platelet; Amine

We evaluated, using a multi-channel electrochemical HPLC system, whether trace amines are detectable in plasma and platelets of healthy control subjects. To this end, levels of tyramine, octopamine and synephrine were assessed in samples obtained from eight males and eight females, age matched and f...