A Bacillus subtilis locus encoding several gene products affecting transport of cations
A 3.6-kb DNA fragment from Bacillus subtilis was found to complement the K + uptake-deficient Escherichia coli strain TK2420. Transformation with a pKLO61 plasmid harboring this fragment conferred the capacity to grow on a minimal medium containing only 10 mM K +. Insertional mutagenesis and subclon...
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Published in | Gene Vol. 188; no. 1; pp. 91 - 94 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
25.03.1997
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Subjects | |
Online Access | Get full text |
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Summary: | A 3.6-kb DNA fragment from
Bacillus subtilis was found to complement the K
+ uptake-deficient
Escherichia coli strain TK2420. Transformation with a pKLO61 plasmid harboring this fragment conferred the capacity to grow on a minimal medium containing only 10 mM K
+. Insertional mutagenesis and subcloning identified a single gene responsible for the complementation. This gene coded for an apparent homolog of
E. coli TrkA. Sequence analysis of the cloned region also revealed three additional open reading frames. These included: a gene encoding a homolog to the
czcD gene product of
Alcaligenes eutrophus, a
lysR-type regulatory gene which was found to enhance Na
+ resistance in
E. coli NM81 (Δ
nhaA) in a separate complementation test, and an
orfD with no significant similarity to sequences deposited in Genbank. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/S0378-1119(96)00784-6 |