Inhibition of cellular RNA methyltransferase abrogates influenza virus capping and replication

• Published in: Science (American Association for the Advancement of Science) Vol. 379; no. 6632; pp. 586 - 591
• Main Authors: Tsukamoto, Yuta, Hiono, Takahiro, Yamada, Shintaro, Matsuno, Keita, Faist, Aileen, Claff, Tobias, Hou, Jianyu, Namasivayam, Vigneshwaran, vom Hemdt, Anja, Sugimoto, Satoko, Ng, Jin Ying, Christensen, Maria H., Tesfamariam, Yonas M., Wolter, Steven, Juranek, Stefan, Zillinger, Thomas, Bauer, Stefan, Hirokawa, Takatsugu, Schmidt, Florian I., Kochs, Georg, Shimojima, Masayuki, Huang, Yi-Shuian, Pichlmair, Andreas, Kümmerer, Beate M., Sakoda, Yoshihiro, Schlee, Martin, Brunotte, Linda, Müller, Christa E., Igarashi, Manabu, Kato, Hiroki
• Format: Journal Article
• Language: English
• Published: United States The American Association for the Advancement of Science 10.02.2023
• Subjects: A549 Cells; Alphainfluenzavirus - drug effects; Animals; Antiviral Agents - chemistry; Antiviral Agents - pharmacology; Betainfluenzavirus - drug effects; Biological Products - chemistry; Biological Products - pharmacology; Computer Simulation; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - pharmacology; Humans; Influenza; Maturation; Methyltransferases - antagonists & inhibitors; Mice; Replication; Ribonucleic acid; RNA; RNA Caps - metabolism; RNA viruses; RNA, Messenger - metabolism; RNA, Viral - biosynthesis; Streptomyces - chemistry; Toxicity; Tubercidin - analogs & derivatives; Tubercidin - pharmacology; Virus Replication - drug effects; Viruses

Orthomyxo- and bunyaviruses steal the 5′ cap portion of host RNAs to prime their own transcription in a process called “cap snatching.” We report that RNA modification of the cap portion by host 2′-O-ribose methyltransferase 1 (MTr1) is essential for the initiation of influenza A and B virus replica...