The mouse uracil-DNA glycosylase gene: isolation of cDNA and genomic clones and mapping ung to mouse chromosome 5

• Published in: Gene Vol. 189; no. 2; pp. 175 - 181
• Main Authors: Svendsen, Pia C, Yee, Harris A, Winkfein, Robert J, van de Sande, Johan H
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 21.04.1997
• Subjects: Amino Acid Sequence; Animals; Base excision; Base Sequence; Chromosome Mapping; Cloning, Molecular; DNA Glycosylases; DNA repair; DNA, Complementary - isolation & purification; Exons; Genes; Humans; In Situ Hybridization, Fluorescence; Introns; Mice; Molecular Sequence Data; Mutation; N-Glycosyl Hydrolases - chemistry; N-Glycosyl Hydrolases - genetics; N-Glycosyl Hydrolases - isolation & purification; Nucleotide sequence; Protein Binding - genetics; Repetitive Sequences, Nucleic Acid; Sequence Analysis, DNA; Transcription, Genetic; Uracil-DNA Glycosidase; aa, amino acid(s); UDG, uracil-DNA glycosylase; ung, mouse gene (DNA) encoding UDG; Nucleotide sequence; FITC, fluorescein isothiocyanate; kb, kilobase(s) or 1000 bp; kDa, kilodalton; bp, base pair(s); p, plasmid; DHFR, dihydrofolate reductase; pfu, plaque forming units; DAPI, 4′,6-diamidino-2-phenylindole; DNA repair; HSV, herpes simplex virus; FISH, fluorescence in situ hybridization; nt, nucleotide(s); Base excision; cDNA, DNA complementary to RNA; PCR, polymerase chain reaction; RACE, rapid amplification of cDNA ends; UNG, human gene (DNA) encoding UDG; Mutation
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(96)00797-4

Uracil-DNA glycosylase (UDG) is the enzyme responsible for the first step in the base-excision repair pathway that specifically removes uracil from DNA. Here we report the isolation of the cDNA and genomic clones for the mouse uracil-DNA glycosylase gene ( ung) homologous to the major placental uracil-DNA glycosylase gene ( UNG) of humans. The complete characterization of the genomic organization of the mouse uracil-DNA glycosylase gene shows that the entire mRNA coding region for the 1.83-kb cDNA of the mouse ung gene is contained in an 8.2-kb SstI genomic fragment which includes six exons and five introns. The cDNA encodes a predicted uracil-DNA glycosylase (UDG) protein of 295 amino acids (33 kDa) that is highly similar to a group of UDGs that have been isolated from a wide variety of organisms. The mouse ung gene has been mapped to mouse chromosome 5 using fluorescence in situ hybridization (FISH).