A comparative study of synchronised and conventional culture methods on the micronucleus dose–response curve

• Published in: Mutation research Vol. 391; no. 1; pp. 91 - 98
• Main Authors: Paul, Solomon F.D, Venkatachalam, P, Jeevanram, R.K
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 13.06.1997
• Subjects: Cell Culture Techniques - methods; Chromosome Aberrations; Cobalt Radioisotopes - toxicity; Conventional culture; Dicentric chromosome; Dose response curve; Dose-Response Relationship, Radiation; Humans; Methotrexate; Methotrexate - pharmacology; Micronucleus; Micronucleus Tests - methods; Mitotic Index; Mutagens - toxicity; Synchronised culture; Synchronised culture; Dicentric chromosome; Methotrexate; Dose response curve; Conventional culture; Micronucleus

Micronucleus (MN) dose–response curves have been studied in blood samples obtained from a healthy volunteer with both a methotrexate synchronised culture and with a conventional culture method. The curve obtained with the synchronised culture, showed better response at higher doses compared to that...