Identification and overexpression of human neutrophil α-defensins (human neutrophil peptides 1, 2 and 3) in squamous cell carcinomas of the human tongue
To date, little attention has been paid to the possible role of α-defensins (human neutrophil peptides 1-3), HNP-1, HNP-2 and HNP-3 in innate host defence against tumour invasion. In the current study, using a single-dimensional high pressure liquid chromatography (HPLC) method for peptide separatio...
Saved in:
| Published in | Oral oncology Vol. 40; no. 2; pp. 139 - 144 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford
Elsevier Ltd
01.02.2004
Elsevier |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | To date, little attention has been paid to the possible role of α-defensins (human neutrophil peptides 1-3), HNP-1, HNP-2 and HNP-3 in innate host defence against tumour invasion. In the current study, using a single-dimensional high pressure liquid chromatography (HPLC) method for peptide separation, followed by mass spectrometry and amino acid sequencing for identification and quantitation, we report the overexpression of HNP-1, HNP-2 and HNP-3 in squamous cell carcinomas of the human tongue compared with autogenous non-tumour tissue. Using a specific antibody we show that the defensins are abundant in neutrophils infiltrating human oral squamous cell carcinoma tissue. In the context of their previously reported oncolytic activity, our results may imply a role for α-defensins in host defence against oral squamous cell carcinoma. |
|---|---|
| AbstractList | To date, little attention has been paid to the possible role of alpha-defensins (human neutrophil peptides 1-3), HNP-1, HNP-2 and HNP-3 in innate host defence against tumour invasion. In the current study, using a single-dimensional high pressure liquid chromatography (HPLC) method for peptide separation, followed by mass spectrometry and amino acid sequencing for identification and quantitation, we report the overexpression of HNP-1, HNP-2 and HNP-3 in squamous cell carcinomas of the human tongue compared with autogenous non-tumour tissue. Using a specific antibody we show that the defensins are abundant in neutrophils infiltrating human oral squamous cell carcinoma tissue. In the context of their previously reported oncolytic activity, our results may imply a role for alpha-defensins in host defence against oral squamous cell carcinoma. To date, little attention has been paid to the possible role of α-defensins (human neutrophil peptides 1-3), HNP-1, HNP-2 and HNP-3 in innate host defence against tumour invasion. In the current study, using a single-dimensional high pressure liquid chromatography (HPLC) method for peptide separation, followed by mass spectrometry and amino acid sequencing for identification and quantitation, we report the overexpression of HNP-1, HNP-2 and HNP-3 in squamous cell carcinomas of the human tongue compared with autogenous non-tumour tissue. Using a specific antibody we show that the defensins are abundant in neutrophils infiltrating human oral squamous cell carcinoma tissue. In the context of their previously reported oncolytic activity, our results may imply a role for α-defensins in host defence against oral squamous cell carcinoma. |
| Author | Gerald Cowan, C. Maxwell, Perry Lundy, Fionnuala T. Gallagher, James R. Lamey, Philip-John Orr, David F. Shaw, Chris Marley, John J. Napier, Seamus S. |
| Author_xml | – sequence: 1 givenname: Fionnuala T. surname: Lundy fullname: Lundy, Fionnuala T. organization: Oral Science Research Centre, School of Dentistry, Queen's University Belfast, BelfastBT12 6BP, N. Ireland, UK – sequence: 2 givenname: David F. surname: Orr fullname: Orr, David F. organization: School of Biomedical Sciences, University of Ulster, Coleraine, N. Ireland, UK – sequence: 3 givenname: James R. surname: Gallagher fullname: Gallagher, James R. organization: Oral Science Research Centre, School of Dentistry, Queen's University Belfast, BelfastBT12 6BP, N. Ireland, UK – sequence: 4 givenname: Perry surname: Maxwell fullname: Maxwell, Perry organization: Department of Histopathology, The Royal Group of Hospitals Trust, Belfast, N. Ireland, UK – sequence: 5 givenname: Chris surname: Shaw fullname: Shaw, Chris organization: School of Biomedical Sciences, University of Ulster, Coleraine, N. Ireland, UK – sequence: 6 givenname: Seamus S. surname: Napier fullname: Napier, Seamus S. organization: Department of Histopathology, The Royal Group of Hospitals Trust, Belfast, N. Ireland, UK – sequence: 7 givenname: C. surname: Gerald Cowan fullname: Gerald Cowan, C. organization: Oral Science Research Centre, School of Dentistry, Queen's University Belfast, BelfastBT12 6BP, N. Ireland, UK – sequence: 8 givenname: Philip-John surname: Lamey fullname: Lamey, Philip-John organization: Oral Science Research Centre, School of Dentistry, Queen's University Belfast, BelfastBT12 6BP, N. Ireland, UK – sequence: 9 givenname: John J. surname: Marley fullname: Marley, John J. email: j.marley@qub.qc.uk organization: Oral Science Research Centre, School of Dentistry, Queen's University Belfast, BelfastBT12 6BP, N. Ireland, UK |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15442315$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/14693236$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkc1u1TAQhS1URNsLjwDyBtRKDdjxT-JVhapCK1ViAawtx55wjRI7tZMKHoXH4EX6TE3uTdUFC1b-mW_mHM05RgchBkDoNSXvKaHyw1fKZF3UrBInhJ0SQnlZ1M_QEa0rVRCh2MF8f0QO0XHOPwkhggryAh1SLhUrmTxCf64dhNG33prRx4BNcDjeQYJfQ4Kcl6_Y4u3Um4ADTGOKw9Z3-P5v4aCFkH3I-OSf8gDD6B1kTM9wuZvJTrEPON9Opo9Txha6DluTrA-xN3nRGLew6owx_JjgJXremi7Dq_XcoO-fLr9dXBU3Xz5fX3y8KSxTdCyUk2ArqWTJmtYaynhVU6No42gjGG9so7hhZVUR0YimEkpyrgSXztZzXUq2Qe_2c4cUbyfIo-59XvyZALNVXRMy97JqBsUetCnmnKDVQ_K9Sb81JXrJRO8y0cvCNWF6l8n82qA3q8DU9OCeutYQZuDtCphsTdcmE6zPT5zgvGRUzNz5noN5HXceks7WQ7DgfAI7ahf9f6w8APv0rII |
| CitedBy_id | crossref_primary_10_1002_jps_21198 crossref_primary_10_3390_ijms12118027 crossref_primary_10_3171_2010_1_FOCUS09300 crossref_primary_10_1158_1078_0432_CCR_05_1538 crossref_primary_10_1021_pr060439b crossref_primary_10_1038_bjc_2017_172 crossref_primary_10_3353_omp_11_1 crossref_primary_10_3390_antibiotics12020361 crossref_primary_10_1369_jhc_2010_956656 crossref_primary_10_1186_1471_2407_10_588 crossref_primary_10_1016_j_cgh_2007_03_013 crossref_primary_10_2165_11597570_000000000_00000 crossref_primary_10_3389_fimmu_2020_00764 crossref_primary_10_1007_s10555_020_09951_1 crossref_primary_10_3390_cancers16040720 crossref_primary_10_1186_s40779_021_00343_2 crossref_primary_10_1111_j_1468_3083_2009_03347_x crossref_primary_10_14216_kjco_16013 crossref_primary_10_1007_s00381_010_1245_4 crossref_primary_10_1007_s00432_012_1303_2 crossref_primary_10_1007_s00432_012_1305_0 crossref_primary_10_1080_02713683_2017_1339806 crossref_primary_10_1096_fj_06_7646com crossref_primary_10_3390_metabo12080704 crossref_primary_10_1111_bpa_12502 crossref_primary_10_3390_ijms18122588 crossref_primary_10_1016_S0242_6498_04_94038_2 crossref_primary_10_1186_1755_8794_1_54 crossref_primary_10_1016_j_oraloncology_2005_11_005 crossref_primary_10_1002_pmic_201300463 crossref_primary_10_1016_j_oraloncology_2012_06_007 crossref_primary_10_1189_jlb_4RU0614_294R crossref_primary_10_1186_1471_2407_5_8 crossref_primary_10_1089_hum_2020_289 crossref_primary_10_1097_MPA_0000000000001054 crossref_primary_10_1038_sj_bjc_6605138 crossref_primary_10_1016_j_jprot_2009_01_002 crossref_primary_10_1080_1061186X_2021_1906885 crossref_primary_10_1021_ac302746t crossref_primary_10_1016_j_ejca_2006_05_039 crossref_primary_10_1053_j_gastro_2005_05_014 crossref_primary_10_3390_biomedicines2020163 crossref_primary_10_3892_or_2013_2947 crossref_primary_10_1177_0960327111412802 crossref_primary_10_1016_j_oraloncology_2018_09_013 crossref_primary_10_1038_sj_onc_1209770 crossref_primary_10_1177_154405910808701011 crossref_primary_10_1016_j_ejca_2005_07_033 crossref_primary_10_1111_prd_12552 crossref_primary_10_1111_omi_12333 |
| Cites_doi | 10.1182/blood.V68.6.1407.1407 10.1016/S0167-7799(97)01156-6 10.1128/AAC.28.5.626 10.1016/0008-8749(88)90258-4 10.1016/S0002-9440(10)62558-8 10.4049/jimmunol.140.8.2686 10.1146/annurev.iy.11.040193.000541 10.1038/309359a0 10.1111/j.1601-0825.1999.tb00078.x 10.1002/1615-9861(200101)1:1<118::AID-PROT118>3.0.CO;2-1 10.1002/(SICI)1098-2744(200003)27:3<158::AID-MC2>3.0.CO;2-2 10.1164/ajrccm/141.1.179 10.1016/0008-8749(91)90136-Y 10.1016/0163-7258(94)00076-F 10.1128/IAI.66.9.4222-4228.1998 |
| ContentType | Journal Article |
| Copyright | 2003 Elsevier Science Ltd 2004 INIST-CNRS |
| Copyright_xml | – notice: 2003 Elsevier Science Ltd – notice: 2004 INIST-CNRS |
| DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
| DOI | 10.1016/S1368-8375(03)00142-8 |
| DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
| DatabaseTitleList | MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Dentistry |
| EISSN | 1879-0593 |
| EndPage | 144 |
| ExternalDocumentID | 10_1016_S1368_8375_03_00142_8 14693236 15442315 S1368837503001428 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- --K --M .1- .55 .FO .GJ .~1 0R~ 123 1B1 1P~ 1RT 1~. 1~5 29N 3O- 4.4 457 4CK 4G. 53G 5RE 5VS 7-5 71M 8P~ AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAWTL AAXUO ABBQC ABFNM ABJNI ABLJU ABLVK ABMAC ABMZM ABOCM ABXDB ABYKQ ACDAQ ACGFS ACIUM ACRLP ADBBV ADEZE AEBSH AEKER AENEX AEVXI AFCTW AFKWA AFRHN AFTJW AFXIZ AGHFR AGUBO AGYEJ AHPSJ AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY AJUYK ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX AXJTR BKOJK BLXMC BNPGV CS3 DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-Q GBLVA HVGLF HZ~ IHE J1W K-O KOM L7B LCYCR M41 MO0 N9A O-L O9- OAUVE OC~ OO- OZT P-8 P-9 P2P PC. Q38 RIG ROL RPZ SCC SDF SDG SDP SEL SES SEW SPCBC SSH SSZ T5K X7M XPP Z5R ZA5 ZGI ~G- 08R AAUGY ABPIF ABPTK IQODW AAXKI AFJKZ AKRWK CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
| ID | FETCH-LOGICAL-c391t-9d6ec769623bfca134781a91bd1b534bcb94a327705b5b7596449546dc8b53663 |
| IEDL.DBID | AIKHN |
| ISSN | 1368-8375 |
| IngestDate | Sat Oct 26 01:45:25 EDT 2024 Fri Dec 06 03:24:50 EST 2024 Sat Sep 28 07:40:48 EDT 2024 Sun Oct 22 16:04:05 EDT 2023 Fri Feb 23 02:12:52 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Keywords | Human OSCC Oral cancer Matrix assisted laser desorption ionisation mass spectrometry MALDI-MS 2D-PAGE 2-dimensional polyacrylamide gel electrophoresis HNP Oral squamous cell carcinoma High pressure liquid chromatography Proteomics Tumour Human defensin Defensin HD HPLC Human neutrophil peptide Squamous cell carcinoma Tongue Peptides Stomatology Granulocyte ENT Malignant tumor Cancerology Oral cavity disease Neutrophil |
| Language | English |
| License | CC BY 4.0 https://www.elsevier.com/tdm/userlicense/1.0 |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c391t-9d6ec769623bfca134781a91bd1b534bcb94a327705b5b7596449546dc8b53663 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 14693236 |
| PQID | 80070537 |
| PQPubID | 23479 |
| PageCount | 6 |
| ParticipantIDs | proquest_miscellaneous_80070537 crossref_primary_10_1016_S1368_8375_03_00142_8 pubmed_primary_14693236 pascalfrancis_primary_15442315 elsevier_sciencedirect_doi_10_1016_S1368_8375_03_00142_8 |
| PublicationCentury | 2000 |
| PublicationDate | 2004-02-01 |
| PublicationDateYYYYMMDD | 2004-02-01 |
| PublicationDate_xml | – month: 02 year: 2004 text: 2004-02-01 day: 01 |
| PublicationDecade | 2000 |
| PublicationPlace | Oxford |
| PublicationPlace_xml | – name: Oxford – name: England |
| PublicationTitle | Oral oncology |
| PublicationTitleAlternate | Oral Oncol |
| PublicationYear | 2004 |
| Publisher | Elsevier Ltd Elsevier |
| Publisher_xml | – name: Elsevier Ltd – name: Elsevier |
| References | Muller, Markovic-Lipkovski, Klatt, Gamper, Schwarz, Beck (BIB5) 2002; 160 Wright, Silverstein (BIB19) 1984; 309 Verhaert, Uttenweiler-Joseph, de Vries, Loboda, Ens, Standing (BIB21) 2001; 1 Lichtenstein, Ganz, Selsted, Lehrer (BIB8) 1986; 68 Sheu, Baldwin, Brunson (BIB10) 1985; 28 Lichtenstein, Ganz, Nguyen, Selsted, Lehrer (BIB11) 1988; 140 Okrent, Lichtenstein, Ganz (BIB9) 1990; 141 Gera, Lichtenstein (BIB7) 1991; 138 Emmert-Buck, Gillespie, Paweletz (BIB20) 2000; 27 Mizukawa, Sawaki, Nagatsuka, Kamio, Yamachika, Fukunaga (BIB17) 2001; 21 Lichtenstein, Ganz, Selsted, Lehrer (BIB12) 1988; 114 Conlon (BIB13) 1997 Hancock, Leher (BIB4) 1998 Mizukawa, Sugiyama, Ueno, Mishima, Takagi, Sugahara (BIB15) 1999; 5 Mizukawa, Sugiyama, Kamio, Yamachika, Ueno, Fukunaga (BIB16) 2000; 20 Leher, Lichtenstein, Ganz (BIB1) 1993 Krisanaprakornkit, Weinberg, Perez, Dale (BIB2) 1998 Mizukawa, Sugiyama, Fukunaga, Ueno, Mishima, Takagi, Sugahara (BIB14) 1998; 18 Ganz, Lehrer (BIB3) 1995; 66 Lehrer, Lichtenstein, Ganz (BIB18) 1993; 11 Barker, Reisfeld (BIB6) 1993; 53 Barker (10.1016/S1368-8375(03)00142-8_BIB6) 1993; 53 Okrent (10.1016/S1368-8375(03)00142-8_BIB9) 1990; 141 Lehrer (10.1016/S1368-8375(03)00142-8_BIB18) 1993; 11 Verhaert (10.1016/S1368-8375(03)00142-8_BIB21) 2001; 1 Mizukawa (10.1016/S1368-8375(03)00142-8_BIB14) 1998; 18 Lichtenstein (10.1016/S1368-8375(03)00142-8_BIB12) 1988; 114 Mizukawa (10.1016/S1368-8375(03)00142-8_BIB15) 1999; 5 Leher (10.1016/S1368-8375(03)00142-8_BIB1) 1993 Muller (10.1016/S1368-8375(03)00142-8_BIB5) 2002; 160 Gera (10.1016/S1368-8375(03)00142-8_BIB7) 1991; 138 Lichtenstein (10.1016/S1368-8375(03)00142-8_BIB8) 1986; 68 Mizukawa (10.1016/S1368-8375(03)00142-8_BIB16) 2000; 20 Ganz (10.1016/S1368-8375(03)00142-8_BIB3) 1995; 66 Hancock (10.1016/S1368-8375(03)00142-8_BIB4) 1998 Conlon (10.1016/S1368-8375(03)00142-8_BIB13) 1997 Krisanaprakornkit (10.1016/S1368-8375(03)00142-8_BIB2) 1998 Sheu (10.1016/S1368-8375(03)00142-8_BIB10) 1985; 28 Wright (10.1016/S1368-8375(03)00142-8_BIB19) 1984; 309 Mizukawa (10.1016/S1368-8375(03)00142-8_BIB17) 2001; 21 Emmert-Buck (10.1016/S1368-8375(03)00142-8_BIB20) 2000; 27 Lichtenstein (10.1016/S1368-8375(03)00142-8_BIB11) 1988; 140 |
| References_xml | – volume: 66 start-page: 191 year: 1995 end-page: 205 ident: BIB3 article-title: Defensins publication-title: Pharmac Ther. contributor: fullname: Lehrer – volume: 160 start-page: 1311 year: 2002 end-page: 1324 ident: BIB5 article-title: Human α-defensins HNPs-1,-2 and-3 in renal cell carcinoma—influences on tumor cell proliferation publication-title: Am J Pathol. contributor: fullname: Beck – volume: 27 start-page: 158 year: 2000 end-page: 165 ident: BIB20 article-title: An approach to proteomic analysis of human tumors publication-title: Mol. Carcinog. contributor: fullname: Paweletz – volume: 28 start-page: 626 year: 1985 end-page: 629 ident: BIB10 article-title: Cyto-toxicity of rabbit macrophage peptides MCP-1 and MCP-2 for mouse-tumor cells publication-title: Antimicrob. Agents Chemother. contributor: fullname: Brunson – volume: 20 start-page: 1125 year: 2000 end-page: 1127 ident: BIB16 article-title: Immunohistochemical staining of human alpha defensin 1 (HNP-1), in the submandibular glands of patients with oral carcinoma publication-title: Anticancer Res. contributor: fullname: Fukunaga – volume: 309 start-page: 359 year: 1984 end-page: 361 ident: BIB19 article-title: Phagocytosing macrophages exclude proteins from the zones of contact with opsonised targets publication-title: Nature contributor: fullname: Silverstein – volume: 53 start-page: 362 year: 1993 end-page: 367 ident: BIB6 article-title: A mechanism for neutrophil-mediated lysis of human neuroblastoma cells publication-title: Cancer Res. contributor: fullname: Reisfeld – volume: 68 start-page: 1407 year: 1986 end-page: 1410 ident: BIB8 article-title: In vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytes publication-title: Blood contributor: fullname: Lehrer – volume: 1 start-page: 118 year: 2001 end-page: 131 ident: BIB21 article-title: Matrix-assisted laser desorption/ionisation quadrupole time of flight mass spectrometry publication-title: Proteomics contributor: fullname: Standing – start-page: 105 year: 1993 end-page: 128 ident: BIB1 article-title: Defensins publication-title: Annu. Rev. Immunol. contributor: fullname: Ganz – volume: 138 start-page: 108 year: 1991 end-page: 120 ident: BIB7 article-title: Human neutrophil peptide defensins induce single-strand DNA breaks in target cells publication-title: Cell. Immunol. contributor: fullname: Lichtenstein – start-page: 4222 year: 1998 end-page: 4228 ident: BIB2 article-title: Expression of the peptide antibiotic human β-defensin 1 in cultured gingival epithelial cells and gingival tissue publication-title: Infect. Immun. contributor: fullname: Dale – volume: 114 start-page: 104 year: 1988 end-page: 116 ident: BIB12 article-title: Synergistic cytolysis mediated by hydrogen peroxide combined with peptide defensins publication-title: Cell. Immunol. contributor: fullname: Lehrer – start-page: 82 year: 1998 end-page: 88 ident: BIB4 article-title: Cationic peptides publication-title: TIBTECH contributor: fullname: Leher – volume: 18 start-page: 4645 year: 1998 end-page: 4650 ident: BIB14 article-title: Defensin-1, a peptide detected in the saliva of oral squamous cell carcimoma patients publication-title: Anticancer Res. contributor: fullname: Sugahara – volume: 21 start-page: 2171 year: 2001 end-page: 2174 ident: BIB17 article-title: Human alpha- and beta- defensin immunoreactivity in oral mucoepidermoid carcinomas publication-title: Anticancer Res. contributor: fullname: Fukunaga – volume: 5 start-page: 139 year: 1999 end-page: 142 ident: BIB15 article-title: Defensin-1, an antimicrobial peptide present in the saliva of patients with oral diseases publication-title: Oral Diseases contributor: fullname: Sugahara – start-page: 1 year: 1997 end-page: 8 ident: BIB13 article-title: Preparation of neuropeptide-containing fractions from biological materials publication-title: Methods in Molecular Biology. Neuropeptide Protocols contributor: fullname: Conlon – volume: 11 start-page: 105 year: 1993 end-page: 128 ident: BIB18 article-title: Defensins—antimicrobial and cytotoxic peptides of mammalian cells publication-title: Annu. Rev. Immunol. contributor: fullname: Ganz – volume: 141 start-page: 179 year: 1990 end-page: 185 ident: BIB9 article-title: Direct cytotoxicity of polymorphonuclear leukocyte granule proteins to human lung-derived cells and endothelaial cells publication-title: Am Rev Respir Dis contributor: fullname: Ganz – volume: 140 start-page: 2686 year: 1988 end-page: 2694 ident: BIB11 article-title: Mechanism of target cytolysis by peptide defensins—target cell metabolic activities, possibly involving endocytosis, are crucial for expression of cytotoxicity publication-title: J. Immunol. contributor: fullname: Lehrer – volume: 53 start-page: 362 year: 1993 ident: 10.1016/S1368-8375(03)00142-8_BIB6 article-title: A mechanism for neutrophil-mediated lysis of human neuroblastoma cells publication-title: Cancer Res. contributor: fullname: Barker – volume: 68 start-page: 1407 year: 1986 ident: 10.1016/S1368-8375(03)00142-8_BIB8 article-title: In vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytes publication-title: Blood doi: 10.1182/blood.V68.6.1407.1407 contributor: fullname: Lichtenstein – start-page: 82 year: 1998 ident: 10.1016/S1368-8375(03)00142-8_BIB4 article-title: Cationic peptides publication-title: TIBTECH doi: 10.1016/S0167-7799(97)01156-6 contributor: fullname: Hancock – volume: 18 start-page: 4645 year: 1998 ident: 10.1016/S1368-8375(03)00142-8_BIB14 article-title: Defensin-1, a peptide detected in the saliva of oral squamous cell carcimoma patients publication-title: Anticancer Res. contributor: fullname: Mizukawa – volume: 28 start-page: 626 year: 1985 ident: 10.1016/S1368-8375(03)00142-8_BIB10 article-title: Cyto-toxicity of rabbit macrophage peptides MCP-1 and MCP-2 for mouse-tumor cells publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.28.5.626 contributor: fullname: Sheu – volume: 114 start-page: 104 year: 1988 ident: 10.1016/S1368-8375(03)00142-8_BIB12 article-title: Synergistic cytolysis mediated by hydrogen peroxide combined with peptide defensins publication-title: Cell. Immunol. doi: 10.1016/0008-8749(88)90258-4 contributor: fullname: Lichtenstein – volume: 160 start-page: 1311 year: 2002 ident: 10.1016/S1368-8375(03)00142-8_BIB5 article-title: Human α-defensins HNPs-1,-2 and-3 in renal cell carcinoma—influences on tumor cell proliferation publication-title: Am J Pathol. doi: 10.1016/S0002-9440(10)62558-8 contributor: fullname: Muller – volume: 140 start-page: 2686 year: 1988 ident: 10.1016/S1368-8375(03)00142-8_BIB11 article-title: Mechanism of target cytolysis by peptide defensins—target cell metabolic activities, possibly involving endocytosis, are crucial for expression of cytotoxicity publication-title: J. Immunol. doi: 10.4049/jimmunol.140.8.2686 contributor: fullname: Lichtenstein – volume: 11 start-page: 105 year: 1993 ident: 10.1016/S1368-8375(03)00142-8_BIB18 article-title: Defensins—antimicrobial and cytotoxic peptides of mammalian cells publication-title: Annu. Rev. Immunol. doi: 10.1146/annurev.iy.11.040193.000541 contributor: fullname: Lehrer – volume: 309 start-page: 359 year: 1984 ident: 10.1016/S1368-8375(03)00142-8_BIB19 article-title: Phagocytosing macrophages exclude proteins from the zones of contact with opsonised targets publication-title: Nature doi: 10.1038/309359a0 contributor: fullname: Wright – volume: 5 start-page: 139 year: 1999 ident: 10.1016/S1368-8375(03)00142-8_BIB15 article-title: Defensin-1, an antimicrobial peptide present in the saliva of patients with oral diseases publication-title: Oral Diseases doi: 10.1111/j.1601-0825.1999.tb00078.x contributor: fullname: Mizukawa – volume: 1 start-page: 118 year: 2001 ident: 10.1016/S1368-8375(03)00142-8_BIB21 article-title: Matrix-assisted laser desorption/ionisation quadrupole time of flight mass spectrometry publication-title: Proteomics doi: 10.1002/1615-9861(200101)1:1<118::AID-PROT118>3.0.CO;2-1 contributor: fullname: Verhaert – volume: 21 start-page: 2171 year: 2001 ident: 10.1016/S1368-8375(03)00142-8_BIB17 article-title: Human alpha- and beta- defensin immunoreactivity in oral mucoepidermoid carcinomas publication-title: Anticancer Res. contributor: fullname: Mizukawa – start-page: 1 year: 1997 ident: 10.1016/S1368-8375(03)00142-8_BIB13 article-title: Preparation of neuropeptide-containing fractions from biological materials contributor: fullname: Conlon – start-page: 105 year: 1993 ident: 10.1016/S1368-8375(03)00142-8_BIB1 article-title: Defensins publication-title: Annu. Rev. Immunol. doi: 10.1146/annurev.iy.11.040193.000541 contributor: fullname: Leher – volume: 27 start-page: 158 year: 2000 ident: 10.1016/S1368-8375(03)00142-8_BIB20 article-title: An approach to proteomic analysis of human tumors publication-title: Mol. Carcinog. doi: 10.1002/(SICI)1098-2744(200003)27:3<158::AID-MC2>3.0.CO;2-2 contributor: fullname: Emmert-Buck – volume: 141 start-page: 179 year: 1990 ident: 10.1016/S1368-8375(03)00142-8_BIB9 article-title: Direct cytotoxicity of polymorphonuclear leukocyte granule proteins to human lung-derived cells and endothelaial cells publication-title: Am Rev Respir Dis doi: 10.1164/ajrccm/141.1.179 contributor: fullname: Okrent – volume: 138 start-page: 108 year: 1991 ident: 10.1016/S1368-8375(03)00142-8_BIB7 article-title: Human neutrophil peptide defensins induce single-strand DNA breaks in target cells publication-title: Cell. Immunol. doi: 10.1016/0008-8749(91)90136-Y contributor: fullname: Gera – volume: 20 start-page: 1125 year: 2000 ident: 10.1016/S1368-8375(03)00142-8_BIB16 article-title: Immunohistochemical staining of human alpha defensin 1 (HNP-1), in the submandibular glands of patients with oral carcinoma publication-title: Anticancer Res. contributor: fullname: Mizukawa – volume: 66 start-page: 191 year: 1995 ident: 10.1016/S1368-8375(03)00142-8_BIB3 article-title: Defensins publication-title: Pharmac Ther. doi: 10.1016/0163-7258(94)00076-F contributor: fullname: Ganz – start-page: 4222 year: 1998 ident: 10.1016/S1368-8375(03)00142-8_BIB2 article-title: Expression of the peptide antibiotic human β-defensin 1 in cultured gingival epithelial cells and gingival tissue publication-title: Infect. Immun. doi: 10.1128/IAI.66.9.4222-4228.1998 contributor: fullname: Krisanaprakornkit |
| SSID | ssj0005150 |
| Score | 1.984844 |
| Snippet | To date, little attention has been paid to the possible role of α-defensins (human neutrophil peptides 1-3), HNP-1, HNP-2 and HNP-3 in innate host defence... To date, little attention has been paid to the possible role of alpha-defensins (human neutrophil peptides 1-3), HNP-1, HNP-2 and HNP-3 in innate host defence... |
| SourceID | proquest crossref pubmed pascalfrancis elsevier |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 139 |
| SubjectTerms | Adult alpha-Defensins - metabolism Amino Acid Sequence Biological and medical sciences Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Chromatography, High Pressure Liquid Defensin Female Human Humans Male Medical sciences Middle Aged Molecular Sequence Data Neoplasm Proteins - metabolism Neoplasm Staging Oral cancer Otorhinolaryngology. Stomatology Proteomics Tongue Neoplasms - metabolism Tongue Neoplasms - pathology Tumors Tumour Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| Title | Identification and overexpression of human neutrophil α-defensins (human neutrophil peptides 1, 2 and 3) in squamous cell carcinomas of the human tongue |
| URI | https://dx.doi.org/10.1016/S1368-8375(03)00142-8 https://www.ncbi.nlm.nih.gov/pubmed/14693236 https://search.proquest.com/docview/80070537 |
| Volume | 40 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB71IQESQlAoLI_FBw6tRLrxOk7iY1WoFlB7gUq9WX4FVqq8obsrceJ_8DP6R_qbmEm8rCpUIXGN44dmxvZn-5sZgDe-cmMvGp_JyoqssIZnihx1a-OEavJGmC6U0slpOTkrPp7L8w04WvnCEK0yrf39mt6t1unLKElz1E6no89clDUerySaaU5xwzZhG7cjeqvdPvzwaXK6Znpw2TsLl3VGFdaOPH0j3ce9XOx37WT1bVvU_dbMUXBNn_HidkjabU3HD-FBwpTssB_2I9gIcQfuviMeEKVy24E7J-kF_TH86j1zm3RVx0z0jFic4UdixEY2a1iXuY_FsFxcztpv0wt2fZX50BDZPc7Z3l_FLXFjfJgz_paNuzbFPptGNv--NHS3wOh9gDnKWxSJkER9IPJM_SD8_LoMT-Ds-P2Xo0mW0jNkqEa-yJQvg6tKhQDKNs6QT2rNjeLWcytFYZ1VhRHjqsqllbaSCqGXkkXpXY3liHR2YSvOYngGzAT8XxTBqoYXxlXKldY7ItAFPAEIMYCDlUZ020fh0H_oaaRCTSrUudCdCnU9gHqlN33DnDTuFP-qOryh53WHskDsyeUAXq8Ur3EukgBNDChLXVPwJCmqATzt7WFdtyhxCojy-f-P6wXc61lDRKV5CVuLy2V4hYBoYYewefCTD5PZ_wYuFQZ3 |
| link.rule.ids | 314,780,784,4502,24116,27924,27925,45585,45679 |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3bbhMxEB2VVqKVEIJyC5fWDzy0EkvW8Xp3_VgVqpQ2eaGV-mb5thAJOWmTSPwKn8GP8E3M7DpEFaqQeF2vL5oZ28f2mRmAt75yAy8an8nKiqywhmeKHHVr44Rq8kaYNpTSaFwOL4tPV_JqA45XvjBEq0xrf7emt6t1-tJP0uzPJpP-Zy7KGo9XEs00p7hh92AL0UCFpr11dHo2HK-ZHlx2zsJlnVGFtSNP10j78SAXh207WX3XFvVgZuYouKbLeHE3JG23ppNH8DBhSnbUDfsxbIS4C9sfiAdEqdx24f4ovaA_gR-dZ26TruqYiZ4RizN8T4zYyKYNazP3sRiWi5vp7OvkG_v1M_OhIbJ7nLODv4pnxI3xYc74OzZo2xSHbBLZ_Hpp6G6B0fsAc5S3KBIhifpA5Jn6Qfj5ZRmewuXJx4vjYZbSM2SoRr7IlC-Dq0qFAMo2zpBPas2N4tZzK0VhnVWFEYOqyqWVtpIKoZeSReldjeWIdJ7BZpzG8AKYCfi_KIJVDS-Mq5QrrXdEoAt4AhCiB-9XGtGzLgqH_kNPIxVqUqHOhW5VqOse1Cu96VvmpHGn-FfVvVt6XncoC8SeXPZgf6V4jXORBGhiQFnqmoInSVH14HlnD-u6RYlTQJQv_39c-7A9vBid6_PT8dkr2OkYRESreQ2bi5tleIPgaGH3kvH_BqGHCHQ |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Identification+and+overexpression+of+human+neutrophil+%CE%B1-defensins+%28human+neutrophil+peptides+1%2C+2+and+3%29+in+squamous+cell+carcinomas+of+the+human+tongue&rft.jtitle=Oral+oncology&rft.au=Lundy%2C+Fionnuala+T.&rft.au=Orr%2C+David+F.&rft.au=Gallagher%2C+James+R.&rft.au=Maxwell%2C+Perry&rft.date=2004-02-01&rft.issn=1368-8375&rft.volume=40&rft.issue=2&rft.spage=139&rft.epage=144&rft_id=info:doi/10.1016%2FS1368-8375%2803%2900142-8&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_S1368_8375_03_00142_8 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1368-8375&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1368-8375&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1368-8375&client=summon |