Crystal Structures of Interleukin-2 Tyrosine Kinase and Their Implications for the Design of Selective Inhibitors
Interleukin-2 tyrosine kinase, Itk, is an important member of the Tec family of non-receptor tyrosine kinases that play a central role in signaling through antigen receptors such as the T-cell receptor, B-cell receptor, and Fcϵ. Selective inhibition of Itk may be an important way of modulating many...
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Published in | The Journal of biological chemistry Vol. 279; no. 18; pp. 18727 - 18732 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Biochemistry and Molecular Biology
30.04.2004
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Subjects | |
Online Access | Get full text |
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Summary: | Interleukin-2 tyrosine kinase, Itk, is an important member of the Tec family of non-receptor tyrosine kinases that play a
central role in signaling through antigen receptors such as the T-cell receptor, B-cell receptor, and Fcϵ. Selective inhibition
of Itk may be an important way of modulating many diseases involving heightened or inappropriate activation of the immune
system. In addition to an unliganded nonphophorylated Itk catalytic kinase domain, we determined the crystal structures of
the phosphorylated and nonphosphorylated kinase domain bound to staurosporine, a potent broad-spectrum kinase inhibitor. These
structures are useful for the design of novel, highly potent and selective Itk inhibitors and provide insight into the influence
of inhibitor binding and phosphorylation on the conformation of Itk. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M400031200 |