Limitations of monoclonal antibodies for monitoring of fungal aerosols using Penicillium brevicompactum as a model fungus

• Published in: Journal of immunological methods Vol. 283; no. 1; pp. 235 - 245
• Main Authors: Schmechel, D., Górny, R.L., Simpson, J.P., Reponen, T., Grinshpun, S.A., Lewis, D.M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.2003; Elsevier
• Subjects: Aerosols; Animals; Antibodies, Monoclonal - immunology; Antibody cross-reactivity; Aspergillus; Biological and medical sciences; Button personal inhalable aerosol sampler; Cross Reactions; ELISA; Enzyme-Linked Immunosorbent Assay - methods; Eurotium; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Fungal aerosol; Mice; Mice, Inbred BALB C; Microbiology; Molecular immunology; Monoclonal antibody (mAb); Mycological methods and techniques used in mycology; Mycology; Penicillium - isolation & purification; Penicillium brevicompactum; Sample processing; Specimen Handling; Spores, Fungal; Techniques; PBS; RT; Sample processing; CCB; PTFE; Button personal inhalable aerosol sampler; Fungal aerosol; mAb; Monoclonal antibody (mAb); Antibody cross-reactivity; Penicillium brevicompactum; OD; PF; ELISA; SIT; Fungi; Models; Fungi Imperfecti; Monoclonal antibody; Thallophyta; Inhalation; Immunological method

Molds are ubiquitous in every environment and many species have been recently associated with an increase in opportunistic infections in immunocompromised patients or the exacerbation of asthmatic episodes in allergic patients. The degree of environmental contamination with fungi thus needs to be mo...