An E-box Motif Residing in the Exon/Intron 1 Junction Regulates Both Transcriptional Activation and Splicing of the Human Norepinephrine Transporter Gene

• Published in: The Journal of biological chemistry Vol. 276; no. 27; pp. 24797 - 24805
• Main Authors: Kim, Chun-Hyung, Ardayfio, Paul, Kim, Kwang-Soo
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 06.07.2001
• Subjects: Base Sequence; Carrier Proteins - genetics; Consensus Sequence; DNA Footprinting; Exons; HeLa Cells; Helix-Loop-Helix Motifs; Humans; Introns; Molecular Sequence Data; Mutagenesis, Site-Directed; NET gene; Norepinephrine; Norepinephrine Plasma Membrane Transport Proteins; norepinephrine transporter; Promoter Regions, Genetic; RNA Splicing; RNA, Messenger - metabolism; sodium chloride; Symporters; Thymidine Kinase - genetics; Thymidine Kinase - metabolism; Transcriptional Activation

The norepinephrine transporter (NET) is responsible for the rapid NaCl-dependent uptake of norepinephrine into presynaptic noradrenergic nerve endings. Recently, we have characterized the structural organization of the 5′ upstream promoter region of the human NET (hNET) gene. A new intron of 476 b...