Single-cell measurement of microbial growth rate with Raman microspectroscopy

• Published in: FEMS microbiology ecology Vol. 100; no. 9
• Main Authors: Caro, Tristan A, Kashyap, Srishti, Brown, George, Chen, Claudia, Kopf, Sebastian H, Templeton, Alexis S
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 13.08.2024
• Subjects: Raman microspectroscopy; growth rate; Stable isotope probing; deuterium; nanoSIMS; single-cell analysis

Rates of microbial growth are fundamental to understanding environmental geochemistry and ecology. However, measuring the heterogeneity of microbial activity at the single-cell level, especially within complex populations and environmental matrices, remains a forefront challenge. Stable isotope prob...