Direct molecular typing of Bordetella pertussis from clinical specimens submitted for diagnostic quantitative (real-time) PCR

• Published in: Journal of medical microbiology Vol. 61; no. 12; pp. 1662 - 1668
• Main Authors: Litt, David J., Jauneikaite, Elita, Tchipeva, Draga, Harrison, Timothy G., Fry, Norman K.
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.12.2012
• Subjects: Bacteriology; Biological and medical sciences; Bordetella Infections - diagnosis; Bordetella pertussis - classification; Bordetella pertussis - genetics; Bordetella pertussis - isolation & purification; DNA Fingerprinting; DNA, Bacterial - analysis; Fundamental and applied biological sciences. Psychology; Humans; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; Molecular Typing; Multilocus Sequence Typing; Real-Time Polymerase Chain Reaction; Polymerase chain reaction; Molecular epidemiology; Bacteria; Genotype; Bordetella pertussis; Real time; Clinical isolate; Quantitative analysis
• ISSN: 0022-2615; 1473-5644; 1473-5644
• DOI: 10.1099/jmm.0.049585-0

Molecular typing of Bordetella pertussis is routinely performed on bacterial isolates, but not on DNA extracted from nasopharyngeal aspirates or pernasal swabs submitted for diagnostic real-time PCR (qPCR). We investigated whether these DNA extracts were suitable for multilocus variable-number tande...