Cloning, expression and regulation of the human S14 gene

The rat S14 gene has been a useful model to study carbohydrate and triiodothyronine (T 3) regulation of hepatic gene expression. To gain insight into the regulation and function of the S14 gene, we isolated the human S14 gene and studied its sequence, tissue specific expression, and transcriptional...

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Published inMolecular and cellular endocrinology Vol. 126; no. 1; pp. 75 - 81
Main Authors Ota, Yasuhiro, Mariash, Ami, Wagner, Jennifer L, Mariash, Cary N
Format Journal Article
LanguageEnglish
Published Ireland Elsevier Ireland Ltd 03.01.1997
Subjects
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Carbohydrate response
Cloning, Molecular
Gene Expression Regulation - drug effects
Glucose - pharmacology
Humans
Liver
Liver - chemistry
Molecular Sequence Data
Nuclear Proteins
Proteins - chemistry
Proteins - genetics
Rats
Restriction Mapping
RNA, Messenger - analysis
S-14 gene (human)
Sequence Homology
Thyroid hormone
Transcription Factors
Transfection
Triiodothyronine - pharmacology
Thyroid hormone
Carbohydrate response
S-14 gene (human)
Liver
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Abstract The rat S14 gene has been a useful model to study carbohydrate and triiodothyronine (T 3) regulation of hepatic gene expression. To gain insight into the regulation and function of the S14 gene, we isolated the human S14 gene and studied its sequence, tissue specific expression, and transcriptional regulation by glucose and T 3. The deduced amino acid sequence of the human S14 protein is 78% identical to that of the rat. Northern blot analysis showed that the S14-mRNA is a single species in human liver and is not present in human brain or HepG2 cells. Transfection studies in primary hepatocytes revealed that transcription of the human S14 gene is regulated by glucose and T 3 in a similar manner to that of the rat gene. However, in HepG2 cells, T 3 and glucose did not affect the transcription of the human S14 gene. These observations suggest that the S14 gene is highly conserved in mammals and is similarly regulated by carbohydrate and T 3 in vivo. More importantly, the function of the human S14 gene may be critical in lipid metabolism in human liver as the rat S14 gene is in rodents.
AbstractList The rat S14 gene has been a useful model to study carbohydrate and triiodothyronine (T3) regulation of hepatic gene expression. To gain insight into the regulation and function of the S14 gene, we isolated the human S14 gene and studied its sequence, tissue specific expression, and transcriptional regulation by glucose and T3. The deduced amino acid sequence of the human S14 protein is 78% identical to that of the rat. Northern blot analysis showed that the S14-mRNA is a single species in human liver and is not present in human brain or HepG2 cells. Transfection studies in primary hepatocytes revealed that transcription of the human S14 gene is regulated by glucose and T3 in a similar manner to that of the rat gene. However, in HepG2 cells, T3 and glucose did not affect the transcription of the human S14 gene. These observations suggest that the S14 gene is highly conserved in mammals and is similarly regulated by carbohydrate and T3 in vivo. More importantly, the function of the human S14 gene may be critical in lipid metabolism in human liver as the rat S14 gene is in rodents.
The rat S14 gene has been a useful model to study carbohydrate and triiodothyronine (T 3) regulation of hepatic gene expression. To gain insight into the regulation and function of the S14 gene, we isolated the human S14 gene and studied its sequence, tissue specific expression, and transcriptional regulation by glucose and T 3. The deduced amino acid sequence of the human S14 protein is 78% identical to that of the rat. Northern blot analysis showed that the S14-mRNA is a single species in human liver and is not present in human brain or HepG2 cells. Transfection studies in primary hepatocytes revealed that transcription of the human S14 gene is regulated by glucose and T 3 in a similar manner to that of the rat gene. However, in HepG2 cells, T 3 and glucose did not affect the transcription of the human S14 gene. These observations suggest that the S14 gene is highly conserved in mammals and is similarly regulated by carbohydrate and T 3 in vivo. More importantly, the function of the human S14 gene may be critical in lipid metabolism in human liver as the rat S14 gene is in rodents.
The rat S14 gene has been a useful model to study carbohydrate and triiodothyronine (T sub(3)) regulation of hepatic gene expression. To gain insight into the regulation and function of the S14 gene, we isolated the human S14 gene and studied its sequence, tissue specific expression, and transcriptional regulation by glucose and T sub(3). The deduced amino acid sequence of the human S14 protein is 78% identical to that of the rat. Northern blot analysis showed that the S14-mRNA is a single species in human liver and is not present in human brain or HepG2 cells. Transfection studies in primary hepatocytes revealed that transcription of the human S14 gene is regulated by glucose and T sub(3) in a similar manner to that of the rat gene. However, in HepG2 cells, T sub(3) and glucose did not affect the transcription of the human S14 gene. These observations suggest that the S14 gene is highly conserved in mammals and is similarly regulated by carbohydrate and T sub(3) in vivo. More importantly, the function of the human S14 gene may be critical in lipid metabolism in human liver as the rat S14 gene is in rodents.
Author Mariash, Ami
Ota, Yasuhiro
Wagner, Jennifer L
Mariash, Cary N
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Issue 1
Keywords Thyroid hormone
Carbohydrate response
S-14 gene (human)
Liver
Language English
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Snippet The rat S14 gene has been a useful model to study carbohydrate and triiodothyronine (T 3) regulation of hepatic gene expression. To gain insight into the...
The rat S14 gene has been a useful model to study carbohydrate and triiodothyronine (T3) regulation of hepatic gene expression. To gain insight into the...
The rat S14 gene has been a useful model to study carbohydrate and triiodothyronine (T sub(3)) regulation of hepatic gene expression. To gain insight into the...
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SubjectTerms Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Carbohydrate response
Cloning, Molecular
Gene Expression Regulation - drug effects
Glucose - pharmacology
Humans
Liver
Liver - chemistry
Molecular Sequence Data
Nuclear Proteins
Proteins - chemistry
Proteins - genetics
Rats
Restriction Mapping
RNA, Messenger - analysis
S-14 gene (human)
Sequence Homology
Thyroid hormone
Transcription Factors
Transfection
Triiodothyronine - pharmacology
Title Cloning, expression and regulation of the human S14 gene
URI https://dx.doi.org/10.1016/S0303-7207(96)03971-8
https://www.ncbi.nlm.nih.gov/pubmed/9027365
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Volume 126
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