Use of Engineered Exosomes Expressing HLA and Costimulatory Molecules to Generate Antigen-specific CD8+ T Cells for Adoptive Cell Therapy

• Published in: Journal of immunotherapy (1997) Vol. 40; no. 3; p. 83
• Main Authors: Kim, Sueon, Sohn, Hyun-Jung, Lee, Hyun-Joo, Sohn, Dae-Hee, Hyun, Seung-Joo, Cho, Hyun-Il, Kim, Tai-Gyu
• Format: Journal Article
• Language: English
• Published: United States 01.04.2017
• Subjects: 4-1BB Ligand - genetics; 4-1BB Ligand - metabolism; Antigens, CD - genetics; Antigens, CD - metabolism; B7-1 Antigen - genetics; B7-1 Antigen - metabolism; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - transplantation; CD83 Antigen; Cross-Priming; Dendritic Cells - pathology; Exosomes - genetics; Exosomes - metabolism; Exosomes - pathology; Genetic Engineering; HLA-A2 Antigen - genetics; HLA-A2 Antigen - metabolism; Humans; Immunoglobulins - genetics; Immunoglobulins - metabolism; Immunotherapy, Adoptive - methods; K562 Cells; Lymphocyte Activation; MART-1 Antigen - immunology; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Neoplasms - immunology; Neoplasms - therapy; Phosphoproteins - immunology; Viral Matrix Proteins - immunology; Virus Diseases - immunology; Virus Diseases - therapy

Dendritic cell-derived exosomes (DEX) comprise an efficient stimulator of T cells. However, the production of sufficient DEX remains a barrier to their broad applicability in immunotherapeutic approaches. In previous studies, genetically engineered K562 have been used to generate artificial antigen...