Research on Chilling Requirements and Physiological Mechanisms of Prunus mume

• Published in: Horticulturae Vol. 9; no. 5; p. 603
• Main Authors: Zhang, Yuhan, Ma, Kaifeng, Li, Qingwei
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 19.05.2023
• Subjects: Abscisic acid; Carbohydrates; Catalase; Chilling; chilling requirement; Cold storage; Cooling; Cultivars; Dormancy; Enzymatic activity; Flowering; Flowers & plants; Fruits; Germination; Gibberellins; Hormones; Humidity; Low level; Low temperature; Osmoregulation; Peroxidase; physiological mechanism of dormancy; Physiology; Prunus mume; Statistical analysis; Superoxide dismutase; Temperature; Temperature requirements; Woody plants; United States > US; China; Utah; Taiwan
• ISSN: 2311-7524; 2311-7524
• DOI: 10.3390/horticulturae9050603

Low temperature plays a vital role in the growth and development of woody plants. In this research, based on the ability of artificial low temperatures to break dormancy, the Utah model was used to determine the chilling requirements (CR) of four early flowering Prunus mume Sieb. et Zucc. (Rosaceae) cultivars, which are widely found in the Henan area of China. In addition, changes in the carbohydrates, antioxidant enzyme activities, and endogenous hormone contents of the flower buds (FBs) of the above-mentioned P. mume cultivars were measured during the low-temperature storage process, and the physiological changes of the four cultivars during the low-temperature induction period were explored. The main research conclusions are as follows: (1) the CRs of ‘Gulihong’, ‘Nanjing gongfen’, ‘Zaoyudie’, and ‘Zaohualve’ were 408CU, 396CU, 372CU, and 348CU, respectively. All the P. mume cultivars belonged to cultivars with a low chilling demand. They also bloomed 4 months earlier, and (2) during the process of releasing dormancy at low temperatures, the contents of soluble sugar (SS) in the osmoregulation system of the four Prunus mume cultivars showed an upward trend, while the content of starch (ST) basically showed an opposite trend to the increase in chilling. The superoxide dismutase (SOD) activity in the FBs of each cultivar gradually decreased with the increase in cold and remained at a low level, while the peroxidase (POD) activity showed an opposite trend, and the dynamic changes of the catalase (CAT) activity generally showed a trend of first increasing and then decreasing. The content of abscisic acid (ABA) showed a trend of increasing first and then decreasing as a whole. The changing trend of gibberellin (GA3) content was similar to that of ABA. In addition, it was found that before dormancy was released, the SS and Pro contents of cultivars with lower CRs and early FB germination were significantly higher than those of other cultivars with higher CRs and the contents of ST and SP were lower. The cultivars with higher CRs and late FB germination had higher ABA content, lower GA3 contents, and their enzyme activities were significantly higher than those of the cultivars with lower CR. Therefore, the changes in the content of various substances in FBs are related to the amount of cooling required by the cultivar and at what point the FBs germinate, and the changes in their contents can be used as one of the indicators for judging the dormancy process of FBs.