Nosocomial transmission of tuberculosis to a nurse demonstrated by means of spoligotyping of a formalin-fixed bronchial biopsy
Background: Tuberculosis was diagnosed in a student nurse. The source of infection was unknown and no positive culture was available. Methods: The diagnosis of tuberculous bronchitis was established on the grounds of a positive Mantoux test, the pathology of a bronchial biopsy and the results of a C...
Saved in:
Published in | Netherlands journal of medicine Vol. 59; no. 3; pp. 152 - 157 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Alphen aan den Rijn
Elsevier B.V
01.09.2001
Van zuiden |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Background: Tuberculosis was diagnosed in a student nurse. The source of infection was unknown and no positive culture was available.
Methods: The diagnosis of tuberculous bronchitis was established on the grounds of a positive Mantoux test, the pathology of a bronchial biopsy and the results of a CT scan of the thorax. Spoligotyping of, for example, formalin-fixed tissue makes it possible to establish the diagnosis in a later phase after all.
Results: Cultures for Mycobacterium tuberculosis were not performed for the student nurse and Ziehl-Neelsen staining of the formalin-fixed bronchial biopsy was negative. The final tuberculosis diagnosis was confirmed by a PCR fingerprint technique, i.e., spoligotyping of a formalin-fixed biopsy specimen. By means of contact investigation and identification of the strain via spoligotyping, comparison of the spoligo patterns made it possible to treat both the patient and those infected by this person correctly.
Conclusions: When there is a pronounced suspicion of tuberculosis and a microbiological culture is not available, it is recommended that supplementary spoligotyping of clinical specimens be carried out. The purpose is to confirm the diagnosis, trace the presumed source case and indirectly to provide information on the drug susceptibility of the relevant M. tuberculosis strain. |
---|---|
AbstractList | BACKGROUNDTuberculosis was diagnosed in a student nurse. The source of infection was unknown and no positive culture was available. METHODSThe diagnosis of tuberculous bronchitis was established on the grounds of a positive Mantoux test, the pathology of a bronchial biopsy and the results of a CT scan of the thorax. Spoligotyping of, for example, formalin-fixed tissue makes it possible to establish the diagnosis in a later phase after all. RESULTSCultures for Mycobacterium tuberculosis were not performed for the student nurse and Ziehl-Neelsen staining of the formalin-fixed bronchial biopsy was negative. The final tuberculosis diagnosis was confirmed by a PCR fingerprint technique, i.e., spoligotyping of a formalin-fixed biopsy specimen. By means of contact investigation and identification of the strain via spoligotyping, comparison of the spoligo patterns made it possible to treat both the patient and those infected by this person correctly. CONCLUSIONSWhen there is a pronounced suspicion of tuberculosis and a microbiological culture is not available, it is recommended that supplementary spoligotyping of clinical specimens be carried out. The purpose is to confirm the diagnosis, trace the presumed source case and indirectly to provide information on the drug susceptibility of the relevant M. tuberculosis strain. Tuberculosis was diagnosed in a student nurse. The source of infection was unknown and no positive culture was available. The diagnosis of tuberculous bronchitis was established on the grounds of a positive Mantoux test, the pathology of a bronchial biopsy and the results of a CT scan of the thorax. Spoligotyping of, for example, formalin-fixed tissue makes it possible to establish the diagnosis in a later phase after all. Cultures for Mycobacterium tuberculosis were not performed for the student nurse and Ziehl-Neelsen staining of the formalin-fixed bronchial biopsy was negative. The final tuberculosis diagnosis was confirmed by a PCR fingerprint technique, i.e., spoligotyping of a formalin-fixed biopsy specimen. By means of contact investigation and identification of the strain via spoligotyping, comparison of the spoligo patterns made it possible to treat both the patient and those infected by this person correctly. When there is a pronounced suspicion of tuberculosis and a microbiological culture is not available, it is recommended that supplementary spoligotyping of clinical specimens be carried out. The purpose is to confirm the diagnosis, trace the presumed source case and indirectly to provide information on the drug susceptibility of the relevant M. tuberculosis strain. Background: Tuberculosis was diagnosed in a student nurse. The source of infection was unknown and no positive culture was available. Methods: The diagnosis of tuberculous bronchitis was established on the grounds of a positive Mantoux test, the pathology of a bronchial biopsy and the results of a CT scan of the thorax. Spoligotyping of, for example, formalin-fixed tissue makes it possible to establish the diagnosis in a later phase after all. Results: Cultures for Mycobacterium tuberculosis were not performed for the student nurse and Ziehl-Neelsen staining of the formalin-fixed bronchial biopsy was negative. The final tuberculosis diagnosis was confirmed by a PCR fingerprint technique, i.e., spoligotyping of a formalin-fixed biopsy specimen. By means of contact investigation and identification of the strain via spoligotyping, comparison of the spoligo patterns made it possible to treat both the patient and those infected by this person correctly. Conclusions: When there is a pronounced suspicion of tuberculosis and a microbiological culture is not available, it is recommended that supplementary spoligotyping of clinical specimens be carried out. The purpose is to confirm the diagnosis, trace the presumed source case and indirectly to provide information on the drug susceptibility of the relevant M. tuberculosis strain. |
Author | van Soolingen, D. Heilmann, F.G.C. Bosje, T. van der Zanden, A.G.M. |
Author_xml | – sequence: 1 givenname: A.G.M. surname: van der Zanden fullname: van der Zanden, A.G.M. email: agm.vd.zanden@wxs.nl organization: Department of Medical Microbiology and Infectious Diseases, Gelre hospitals, Location Lukas, P.O. Box 9014, 7300 DS Apeldoorn, The Netherlands – sequence: 2 givenname: T. surname: Bosje fullname: Bosje, T. organization: Department for the Prevention of Tuberculosis, Public Health Authority Groningen, Groningen, The Netherlands – sequence: 3 givenname: F.G.C. surname: Heilmann fullname: Heilmann, F.G.C. organization: Department of Medical Microbiology and Infectious Diseases, Gelre hospitals, Location Lukas, P.O. Box 9014, 7300 DS Apeldoorn, The Netherlands – sequence: 4 givenname: D. surname: van Soolingen fullname: van Soolingen, D. organization: Mycobacterium Department, National Institute for Public Health and the Environment, Laboratory for Diagnostics of Infectious Diseases and Screening, Bilthoven, The Netherlands |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14074951$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/11583831$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkE1v1DAQhi1URLeFnwDyBVQOAU_srJ0TQhUFpAoOgNSbZTuTYpTYiydB7IXfTtJd0SOnkUbPOx_PGTtJOSFjT0G8AgHb11-EFKKqW60vBLwUAhpZ3TxgGzC6rlqxhRO2-YecsjOiH0KIrW6bR-wUoDHSSNiwP58y5ZDH6AY-FZdojEQxJ557Ps0eS5iHTJH4lLnjaS6EvMMxJ1roCTvu93zEJbcGaJeHeJun_S6m27XheJ_L6IaYqj7-XumSU_i-LvMx72j_mD3s3UD45FjP2berd18vP1TXn99_vHx7XQXZwlSBrztVC1drqZbXgtLBexW8EQKh9bpRfdOg8boLKJ2qDepgWoDaiGBk6OU5e3GYuyv554w02eXPgMPgEuaZrIYapFFqAZsDGEomKtjbXYmjK3sLwq7i7Z14u1q1AuydeHuz5J4dF8x-xO4-dTS9AM-PgKPghn5xHSLdc0po1TYr9-bA4aLjV8RiKURMAbtYMEy2y_E_p_wFNUikIA |
CitedBy_id | crossref_primary_10_1128_JCM_40_12_4628_4639_2002 crossref_primary_10_1186_1471_2334_14_409 crossref_primary_10_1128_JCM_41_11_5350_5351_2003 crossref_primary_10_1371_journal_pone_0121639 crossref_primary_10_1007_s00508_006_0721_z |
ContentType | Journal Article |
Copyright | 2001 Elsevier Science B.V. 2002 INIST-CNRS |
Copyright_xml | – notice: 2001 Elsevier Science B.V. – notice: 2002 INIST-CNRS |
DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.1016/S0300-2977(01)00153-X |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine |
EISSN | 1872-9061 |
EndPage | 157 |
ExternalDocumentID | 10_1016_S0300_2977_01_00153_X 11583831 14074951 S030029770100153X |
Genre | Journal Article Case Reports |
GroupedDBID | --- --K 0R~ 123 1B1 29M 2WC 53G 5VS AAEDT AALRI AAQFI AAQXK AAXUO ABMAC ADBBV ADMUD AENEX ALMA_UNASSIGNED_HOLDINGS BAWUL CS3 DIK DU5 EBS EJD F5P FDB FEDTE FGOYB FRP GX1 HVGLF HZ~ IHE M41 NQ- O9- OK1 P6G R2- RIG ROL RPZ SEW TR2 UHS W2D ZGI IPNFZ IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-c391t-1b2d420a2734061c47cbb4cb800e19b754f55e8b7dce3a428e7c8911280c83cf3 |
ISSN | 0300-2977 |
IngestDate | Fri Aug 16 11:35:47 EDT 2024 Fri Aug 23 01:51:26 EDT 2024 Sat Sep 28 07:36:29 EDT 2024 Thu Nov 24 18:22:07 EST 2022 Fri Feb 23 02:39:30 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Keywords | Mycobacterium tuberculosis ZN Nurses PCR RFLP Human Surgical biopsy Nosocomial infection Radiodiagnosis Respiratory disease Transmission Bronchus Spoligotyping Mycobacterial infection Infection Case study Polymerase chain reaction Tuberculosis Surgery Young adult Bacteriosis Bronchus disease Medical imagery Female Computerized axial tomography Diagnosis Molecular biology Nurse |
Language | English |
License | CC BY 4.0 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c391t-1b2d420a2734061c47cbb4cb800e19b754f55e8b7dce3a428e7c8911280c83cf3 |
Notes | ObjectType-Case Study-2 SourceType-Scholarly Journals-1 ObjectType-Feature-4 content type line 23 ObjectType-Report-1 ObjectType-Article-3 |
PMID | 11583831 |
PQID | 71213844 |
PQPubID | 23479 |
PageCount | 6 |
ParticipantIDs | proquest_miscellaneous_71213844 crossref_primary_10_1016_S0300_2977_01_00153_X pubmed_primary_11583831 pascalfrancis_primary_14074951 elsevier_sciencedirect_doi_10_1016_S0300_2977_01_00153_X |
PublicationCentury | 2000 |
PublicationDate | 2001-09-01 |
PublicationDateYYYYMMDD | 2001-09-01 |
PublicationDate_xml | – month: 09 year: 2001 text: 2001-09-01 day: 01 |
PublicationDecade | 2000 |
PublicationPlace | Alphen aan den Rijn |
PublicationPlace_xml | – name: Alphen aan den Rijn – name: Netherlands |
PublicationTitle | Netherlands journal of medicine |
PublicationTitleAlternate | Neth J Med |
PublicationYear | 2001 |
Publisher | Elsevier B.V Van zuiden |
Publisher_xml | – name: Elsevier B.V – name: Van zuiden |
References | Behr, Warren, Salamon (BIB10) 1999; 353 Kiers, Drost, Van Soolingen, Veen (BIB2) 1996; 140 Kamerbeek, Schouls, Kolk (BIB8) 1997; 35 Gordin, Chaisson, Matts (BIB12) 2000; 283 Koninklijke Nederlandse Centrale Vereniging tot bestrijding der Tuberculose. Jaarverslag 1999. Den Haag, 2000. Sepkowitz (BIB9) 1996; 23 Lambregts-van Weezenbeek, Keizer, Sebek, Schepp-Beelen, Van der Loo (BIB3) 1996; 140 Crofton, Horne, Miller (BIB4) 2000 Van der Zanden, Hoentjen, Heilmann, Weltevreden, Schouls, Van Embden (BIB7) 1998; 51 Veen (BIB5) 1992; 73 Alito, Morcillo, Scipioni, Dolmann, Romano, Cataldi, van Soolingen (BIB13) 1999; 37 Van Embden, Cave, Crawford (BIB6) 1993; 31 American Thoracic Society. Targeted tuberculin testing and treatment of latent tuberculosis infection. MMWR Morbid Mortal Wkly Rep 2000;49:1–51. |
References_xml | – volume: 51 start-page: 209 year: 1998 end-page: 214 ident: BIB7 article-title: Simultaneous detection and strain differentiation of publication-title: Mol Pathol contributor: fullname: Van Embden – volume: 23 start-page: 954 year: 1996 end-page: 962 ident: BIB9 article-title: How contagious is tuberculosis? publication-title: Clin Infect Dis contributor: fullname: Sepkowitz – volume: 37 start-page: 788 year: 1999 end-page: 791 ident: BIB13 article-title: The IS6110 restriction fragment length polymorphism in particular multidrug-resistant publication-title: J Clin Microbiol contributor: fullname: van Soolingen – volume: 73 start-page: 73 year: 1992 end-page: 76 ident: BIB5 article-title: Microepidemics of tuberculosis: the stone-in-the-pond principle publication-title: Tubercle Lung Dis contributor: fullname: Veen – year: 2000 ident: BIB4 publication-title: Clinical Tuberculosis contributor: fullname: Miller – volume: 283 start-page: 1445 year: 2000 end-page: 1450 ident: BIB12 article-title: Rifampin and pyrazinamide vs. isoniazid for prevention of tuberculosis in HIV-infected persons: an international randomized trial. Terry Beirn Community Programs for Clinical Research on AIDS, the Adult AIDS Clinical Trials Group, the Pan American Health Organization, and the Centers for Disease Control and Prevention Study Group publication-title: J Am Med Assoc contributor: fullname: Matts – volume: 353 start-page: 444 year: 1999 end-page: 449 ident: BIB10 article-title: Transmission of publication-title: Lancet contributor: fullname: Salamon – volume: 140 start-page: 2290 year: 1996 end-page: 2293 ident: BIB2 article-title: [Border-crossing source tracing in tuberculosis via DNA fingerprint technique] publication-title: Ned Tijdschr Geneeskd contributor: fullname: Veen – volume: 140 start-page: 2293 year: 1996 end-page: 2295 ident: BIB3 article-title: Transmission of multiresistant tuberculosis in a Dutch hospital publication-title: Ned Tijdschr Geneeskd contributor: fullname: Van der Loo – volume: 31 start-page: 406 year: 1993 end-page: 409 ident: BIB6 article-title: Strain identification of publication-title: J Clin Microbiol contributor: fullname: Crawford – volume: 35 start-page: 907 year: 1997 end-page: 914 ident: BIB8 article-title: Simultaneous detection and strain differentiation of publication-title: J Clin Microbiol contributor: fullname: Kolk |
SSID | ssj0006795 |
Score | 1.642334 |
Snippet | Background: Tuberculosis was diagnosed in a student nurse. The source of infection was unknown and no positive culture was available.
Methods: The diagnosis of... Tuberculosis was diagnosed in a student nurse. The source of infection was unknown and no positive culture was available. The diagnosis of tuberculous... BACKGROUNDTuberculosis was diagnosed in a student nurse. The source of infection was unknown and no positive culture was available. METHODSThe diagnosis of... |
SourceID | proquest crossref pubmed pascalfrancis elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 152 |
SubjectTerms | Bacterial diseases Biological and medical sciences Biopsy - methods Bronchitis - diagnosis Bronchitis - drug therapy Bronchitis - microbiology Cross Infection - diagnosis Cross Infection - drug therapy Cross Infection - microbiology Cross Infection - transmission Diagnosis, Differential DNA Fingerprinting DNA, Bacterial - analysis DNA, Bacterial - genetics Female Human bacterial diseases Humans Infectious Disease Transmission, Patient-to-Professional - methods Infectious diseases Medical sciences Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Nurses PCR Polymorphism, Genetic RFLP Serotyping - methods Students, Nursing Tomography, X-Ray Computed Tropical medicine Tuberculosis and atypical mycobacterial infections Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - drug therapy Tuberculosis, Pulmonary - microbiology Tuberculosis, Pulmonary - transmission |
Title | Nosocomial transmission of tuberculosis to a nurse demonstrated by means of spoligotyping of a formalin-fixed bronchial biopsy |
URI | https://dx.doi.org/10.1016/S0300-2977(01)00153-X https://www.ncbi.nlm.nih.gov/pubmed/11583831 https://search.proquest.com/docview/71213844 |
Volume | 59 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwGLXKkBASQtwpl-EHHkBTQtwkTfKIJrYKqXvZJkW8RLbjTEVtXTWpxHjgp_Hb-D7bTVLGBOwlqixfqpwT-9j-LoS8jatYqKBknuDj0IsyWXppWUkvUQA_KISyMul8pifjyXn0OY_zweBnz2pp0whffv-jX8lNUIUywBW9ZP8D2bZTKIDfgC88AWF4_hPGJxrerV4Y1w9ccwCz2gnAZiPUWm7mGgOOgL7k1lzjoFQLVIQYIMJIz4Xi1hCuxnQNF7q5XDk7aG4dG0GGetXsG9Ze6yVaRs8PxEyv6p374J7fcD8Yxe839-gshcErvuDRtQ1e4B_7U789FND1VxsGuC2aqNl84TI5H0HlQ7_f2anGpEMXbu70d84wWGuk5Q7WrjjXWIeuIPBGmcvyouz8nCYjLwts_PbtBO5Cis_6-3szGzMbHNct7MxGwr6yZtjji9N2OFD2mL0gQzkZenm3ULbmi6YuVg2YqZTfIrdHSRajSelx3pkYjROT9aftufMg-9AN9y5g791Q12mjeytewxdb2VQr1--FjCY6e0Duu80M_WiZ-ZAM1PIRuTN1oD8mPzqC0j5Bqa5on6C00ZRTQ1DaJygVl9QQFBvsEBQLON0lKG0JSi1Bn5Dzo09nhxPPJfzwZJixxmNiVEajgGPIJcBYRokUIpICNjWKZQLmjiqOVSqSUqqQw8ZZJTKF1RoAk2koq_Ap2VvqpXpOKBPjTKiYyywIo3GV8ZKVXCrJo0yEUcqHxN--6mJl47oUncEjXvEiNkXACoNNkQ9JugWkcOLUis4CePS3pvs7AHYDRiDgYYszJG-2iBYABF7Z8aXSm7pIMOJiGkVD8swC3bVlaPAQshc3_18vyd3uY3xF9pr1Rr0Gid2IfcPjX6Fiz4s |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Geneva Foundation for Medical Education and Research |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Nosocomial+transmission+of+tuberculosis+to+a+nurse+demonstrated+by+means+of+spoligotyping+of+a+formalin-fixed+bronchial+biopsy&rft.jtitle=Netherlands+journal+of+medicine&rft.au=van+der+Zanden%2C+A.G.M.&rft.au=Bosje%2C+T.&rft.au=Heilmann%2C+F.G.C.&rft.au=van+Soolingen%2C+D.&rft.date=2001-09-01&rft.pub=Elsevier+B.V&rft.issn=0300-2977&rft.eissn=1872-9061&rft.volume=59&rft.issue=3&rft.spage=152&rft.epage=157&rft_id=info:doi/10.1016%2FS0300-2977%2801%2900153-X&rft.externalDocID=S030029770100153X |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0300-2977&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0300-2977&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0300-2977&client=summon |