Mutant combinations of lycopene ɛ‐cyclase and β‐carotene hydroxylase 2 homoeologs increased β‐carotene accumulation in endosperm of tetraploid wheat (Triticum turgidum L.) grains

• Published in: Plant biotechnology journal Vol. 20; no. 3; pp. 564 - 576
• Main Authors: Yu, Shu, Li, Michelle, Dubcovsky, Jorge, Tian, Li
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.03.2022; John Wiley and Sons Inc
• Subjects: Amino acids; Anodontia; beta Carotene - genetics; beta Carotene - metabolism; biofortification; Biosynthesis; biotechnology; Carotene; carotenoid; Carotenoids; Carotenoids - metabolism; Catalysis; catalytic activity; Comparative analysis; Endosperm; Endosperm - genetics; Endosperm - metabolism; Ferredoxin hydrogenase; flour; Gene expression; genes; Genetic modification; Genomes; Grain; hexaploidy; Hydroxylase; Intramolecular Lyases - genetics; Intramolecular Lyases - metabolism; Light effects; loss-of-function mutation; Lutein; Lutein - metabolism; Lycopene; Lycopene - metabolism; lycopene ɛ‐cyclase; Metabolism; Mixed Function Oxygenases - genetics; Mixed Function Oxygenases - metabolism; Mutants; Mutation; Photosynthesis; provitamin A biofortification; Provitamins - metabolism; Tetraploidy; Triticum - genetics; Triticum - metabolism; Triticum turgidum; Wheat; Xanthophylls; Xanthophylls - metabolism; β-Carotene; β‐carotene hydroxylase; β-carotene hydroxylase; provitamin A biofortification; β-carotene; carotenoid; grain; endosperm; Wheat; lutein; lycopene ɛ-cyclase

Summary Grains of tetraploid wheat (Triticum turgidum L.) mainly accumulate the non‐provitamin A carotenoid lutein—with low natural variation in provitamin A β‐carotene in wheat accessions necessitating alternative strategies for provitamin A biofortification. Lycopene ɛ‐cyclase (LCYe) and β‐caroten...