Rapid probe and isolation of bioactive compounds from Dioscorea panthaica using human serum albumin functionalized magnetic nano-particles (HSA-MNPs)-based ligand fishing coupled with electrospray ionization mass spectrometry
The chemical diversity of secondary metabolites in medicinal plant makes it a huge challenge to isolate the bioactive compounds from herbal extracts, so quick recognition of the bioactive ones is of vital importance for improving the efficiency of isolation. In this study, a ligand fishing experimen...
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Published in | Rapid communications in mass spectrometry Vol. 24; no. 22; pp. 3335 - 3339 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
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Chichester, UK
John Wiley & Sons, Ltd
30.11.2010
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Abstract | The chemical diversity of secondary metabolites in medicinal plant makes it a huge challenge to isolate the bioactive compounds from herbal extracts, so quick recognition of the bioactive ones is of vital importance for improving the efficiency of isolation. In this study, a ligand fishing experiment based on human serum albumin functionalized magnetic nano‐particles (HSA‐MNPs) was performed to probe the bioactive components in a traditional Chinese medicinal plant, Dioscorea panthaica. The minor compounds fished out by HSA‐MNPs were identified by electrospray ionization mass spectrometry (ESI‐MS), and then separated from the extract of the whole plant by one or two steps of column chromatography under the guidance of ESI‐MS. Four biologically active compounds, progenin II, progenin III, dioscin and gracillin, were isolated much faster than in the normal lengthy phytochemical procedure. The present study demonstrates that biological macromolecule (protein, enzyme, receptor, et al.) functionalized MNPs may serve as baits to recognize bioactive small molecules in complex herbal extracts. It is expected that a macromolecule functionalized MNPs‐based ligand fishing experiment coupled with ESI‐MS may accelerate the process of identification and isolation of bioactive components from medicinal plants, and thus benefit the speed of drug discovery. Copyright © 2010 John Wiley & Sons, Ltd. |
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AbstractList | The chemical diversity of secondary metabolites in medicinal plant makes it a huge challenge to isolate the bioactive compounds from herbal extracts, so quick recognition of the bioactive ones is of vital importance for improving the efficiency of isolation. In this study, a ligand fishing experiment based on human serum albumin functionalized magnetic nano‐particles (HSA‐MNPs) was performed to probe the bioactive components in a traditional Chinese medicinal plant, Dioscorea panthaica. The minor compounds fished out by HSA‐MNPs were identified by electrospray ionization mass spectrometry (ESI‐MS), and then separated from the extract of the whole plant by one or two steps of column chromatography under the guidance of ESI‐MS. Four biologically active compounds, progenin II, progenin III, dioscin and gracillin, were isolated much faster than in the normal lengthy phytochemical procedure. The present study demonstrates that biological macromolecule (protein, enzyme, receptor, et al.) functionalized MNPs may serve as baits to recognize bioactive small molecules in complex herbal extracts. It is expected that a macromolecule functionalized MNPs‐based ligand fishing experiment coupled with ESI‐MS may accelerate the process of identification and isolation of bioactive components from medicinal plants, and thus benefit the speed of drug discovery. Copyright © 2010 John Wiley & Sons, Ltd. The chemical diversity of secondary metabolites in medicinal plant makes it a huge challenge to isolate the bioactive compounds from herbal extracts, so quick recognition of the bioactive ones is of vital importance for improving the efficiency of isolation. In this study, a ligand fishing experiment based on human serum albumin functionalized magnetic nano-particles (HSA-MNPs) was performed to probe the bioactive components in a traditional Chinese medicinal plant, Dioscorea panthaica. The minor compounds fished out by HSA-MNPs were identified by electrospray ionization mass spectrometry (ESI-MS), and then separated from the extract of the whole plant by one or two steps of column chromatography under the guidance of ESI-MS. Four biologically active compounds, progenin II, progenin III, dioscin and gracillin, were isolated much faster than in the normal lengthy phytochemical procedure. The present study demonstrates that biological macromolecule (protein, enzyme, receptor, et al.) functionalized MNPs may serve as baits to recognize bioactive small molecules in complex herbal extracts. It is expected that a macromolecule functionalized MNPs-based ligand fishing experiment coupled with ESI-MS may accelerate the process of identification and isolation of bioactive components from medicinal plants, and thus benefit the speed of drug discovery. Abstract The chemical diversity of secondary metabolites in medicinal plant makes it a huge challenge to isolate the bioactive compounds from herbal extracts, so quick recognition of the bioactive ones is of vital importance for improving the efficiency of isolation. In this study, a ligand fishing experiment based on human serum albumin functionalized magnetic nano‐particles (HSA‐MNPs) was performed to probe the bioactive components in a traditional Chinese medicinal plant, Dioscorea panthaica . The minor compounds fished out by HSA‐MNPs were identified by electrospray ionization mass spectrometry (ESI‐MS), and then separated from the extract of the whole plant by one or two steps of column chromatography under the guidance of ESI‐MS. Four biologically active compounds, progenin II, progenin III, dioscin and gracillin, were isolated much faster than in the normal lengthy phytochemical procedure. The present study demonstrates that biological macromolecule (protein, enzyme, receptor, et al .) functionalized MNPs may serve as baits to recognize bioactive small molecules in complex herbal extracts. It is expected that a macromolecule functionalized MNPs‐based ligand fishing experiment coupled with ESI‐MS may accelerate the process of identification and isolation of bioactive components from medicinal plants, and thus benefit the speed of drug discovery. Copyright © 2010 John Wiley & Sons, Ltd. |
Author | Xue, Ying Shan, Xiao-Qing Deng, Wen-Long Xu, Xue-Min Li, Bo-Gang Liao, Xun Qing, Lin-Sen Wang, Xiao-Ling |
Author_xml | – sequence: 1 givenname: Lin-Sen surname: Qing fullname: Qing, Lin-Sen organization: National Engineering Research Center for Natural Medicines, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, P.R. China – sequence: 2 givenname: Xiao-Qing surname: Shan fullname: Shan, Xiao-Qing organization: Southwest University for Nationalities, Chengdu 610041, P.R. China – sequence: 3 givenname: Xue-Min surname: Xu fullname: Xu, Xue-Min organization: Sichuan Key Laboratory of Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, P.R. China – sequence: 4 givenname: Ying surname: Xue fullname: Xue, Ying organization: National Engineering Research Center for Natural Medicines, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, P.R. China – sequence: 5 givenname: Wen-Long surname: Deng fullname: Deng, Wen-Long organization: Sichuan Key Laboratory of Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, P.R. China – sequence: 6 givenname: Bo-Gang surname: Li fullname: Li, Bo-Gang organization: National Engineering Research Center for Natural Medicines, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, P.R. China – sequence: 7 givenname: Xiao-Ling surname: Wang fullname: Wang, Xiao-Ling organization: Southwest University for Nationalities, Chengdu 610041, P.R. China – sequence: 8 givenname: Xun surname: Liao fullname: Liao, Xun email: liaoxun@cib.ac.cn organization: National Engineering Research Center for Natural Medicines, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, P.R. China |
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Cites_doi | 10.1016/0022-1759(93)90101-C 10.1021/ac070268 10.1016/S0040-4020(00)01024-3 10.1016/0167-4838(96)00013-1 10.1021/ac9000755 10.1016/j.chroma.2010.05.009 10.1021/ac070195u 10.1002/1521-3773(20020802)41:15<2644::AID-ANIE2644>3.0.CO;2-O 10.1002/1521-3773(20020215)41:4<646::AID-ANIE646>3.0.CO;2-I 10.1021/ac049335f 10.1021/ac070146h 10.1016/j.bbrc.2006.01.069 10.1021/ac010569y 10.1021/ac801153h 10.1021/ac034794m 10.1002/jms.988 |
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References | Gohlke H, Klebe G. Angew. Chem. Int. Ed. Engl. 2002; 41: 2644. Zou HF, Zhang QC, Guo Z, Guo BC, Zhang Q, Chen XM. Angew. Chem. Int. Ed. Engl. 2002; 41: 646. Marszałł MP, Moaddel R, Kole S, Gandhari M, Bernier M, Wainer IW. Anal. Chem. 2008; 80: 7571. Jiangsu New Medical College. Chinese Materia Medica Dictionary. Shanghai Press of Science and Technology: Shanghai, 1977. Fermas S, Gonnet F, Varenne A, Gareil P, Daniel R. Anal. Chem. 2007; 79: 4987. Geng Y, Tan NH, Zhou J, Kong LY. Chin. J. Nat. Med. 2004; 2: 25. Brenner GM, Stevens CW. Pharmacology, (2nd edn). Elsevier: Philadelphia, 2006. Widjojoatmodjo MN, Fluit AC, Torensma R, Verhoef J. J. Immunol. Methods 1993; 165: 11. Jonker N, Kretschmer A, Kool J, Fernandez A, Kloos D, Krabbe JG, Lingeman H, Irth H. Anal. Chem. 2009; 81: 4263. Yamasaki K, Maruyama T, Kragh-Hansen U, Otagiria M. Biochim. Biophys. Acta 1996; 1295: 147. Sugo T, Tachimoto H, Murakami TY. Biochem. Biophys. Res. Commun. 2006; 341: 1078. Lin PC, Tseng MC, Su AK, Chen YJ, Lin CC. Anal. Chem. 2007; 79: 3401. Moaddel R, Marsza MP, Bighi F, Yang Q, Duan X, Wainer IW. Anal. Chem. 2007; 79: 5414. Qing LS, Xue Y, Zheng Y, Xiong J, Liao X, Ding LS, Li BG, Liu YM. J. Chromatogr. A 2010; 1217: 4663. Kelly MA, McLellan TJ, Rosner PJ. Anal. Chem. 2001; 74: 1. Li R, Zhou Y, Wu ZJ, Ding LS. J. Mass Spectrom. 2006; 41: 1. Ferrer I, Heine CE, Thurman EM. Anal. Chem. 2004; 76: 1437. Li BG, Tang YF, Shi Y. Bull. Bot. 1986; 28: 409. Dong M, Feng XZ, Wang BX, Wu LJ, Ikejima T. Tetrahedron 2001; 57: 501. Borch J, Roepstorff P. Anal. Chem. 2004; 76: 5243. 2004; 76 1996; 1295 2006; 41 2002; 41 2009; 81 2010; 1217 1986; 28 2006; 341 2006 2004; 2 2001; 57 2007; 79 1993; 165 2008; 80 2001; 74 1977 Brenner GM (e_1_2_6_2_2) 2006 Geng Y (e_1_2_6_19_2) 2004; 2 e_1_2_6_8_2 e_1_2_6_7_2 Jiangsu New Medical College (e_1_2_6_17_2) 1977 e_1_2_6_18_2 e_1_2_6_9_2 e_1_2_6_4_2 e_1_2_6_3_2 Li BG (e_1_2_6_20_2) 1986; 28 e_1_2_6_6_2 e_1_2_6_5_2 e_1_2_6_12_2 e_1_2_6_13_2 e_1_2_6_10_2 e_1_2_6_11_2 e_1_2_6_21_2 e_1_2_6_16_2 e_1_2_6_14_2 e_1_2_6_15_2 |
References_xml | – volume: 2 start-page: 25 year: 2004 publication-title: Chin. J. Nat. Med. – volume: 1295 start-page: 147 year: 1996 publication-title: Biochim. Biophys. Acta – volume: 41 start-page: 646 year: 2002 publication-title: Angew. Chem. Int. Ed. Engl. – volume: 74 start-page: 1 year: 2001 publication-title: Anal. Chem. – year: 2006 – volume: 80 start-page: 7571 year: 2008 publication-title: Anal. Chem. – volume: 41 start-page: 1 year: 2006 publication-title: J. Mass Spectrom. – volume: 41 start-page: 2644 year: 2002 publication-title: Angew. Chem. Int. Ed. Engl. – volume: 341 start-page: 1078 year: 2006 publication-title: Biochem. Biophys. Res. Commun. – volume: 76 start-page: 5243 year: 2004 publication-title: Anal. Chem. – volume: 28 start-page: 409 year: 1986 publication-title: Bull. Bot. – volume: 81 start-page: 4263 year: 2009 publication-title: Anal. Chem. – volume: 1217 start-page: 4663 year: 2010 publication-title: J. Chromatogr. A – volume: 165 start-page: 11 year: 1993 publication-title: J. Immunol. Methods – year: 1977 – volume: 57 start-page: 501 year: 2001 publication-title: Tetrahedron – volume: 79 start-page: 5414 year: 2007 publication-title: Anal. Chem. – volume: 76 start-page: 1437 year: 2004 publication-title: Anal. Chem. – volume: 79 start-page: 4987 year: 2007 publication-title: Anal. Chem. – volume: 79 start-page: 3401 year: 2007 publication-title: Anal. Chem. – ident: e_1_2_6_8_2 doi: 10.1016/0022-1759(93)90101-C – volume-title: Pharmacology year: 2006 ident: e_1_2_6_2_2 contributor: fullname: Brenner GM – ident: e_1_2_6_11_2 doi: 10.1021/ac070268 – ident: e_1_2_6_18_2 doi: 10.1016/S0040-4020(00)01024-3 – ident: e_1_2_6_16_2 doi: 10.1016/0167-4838(96)00013-1 – ident: e_1_2_6_9_2 doi: 10.1021/ac9000755 – ident: e_1_2_6_15_2 doi: 10.1016/j.chroma.2010.05.009 – ident: e_1_2_6_10_2 doi: 10.1021/ac070195u – ident: e_1_2_6_3_2 doi: 10.1002/1521-3773(20020802)41:15<2644::AID-ANIE2644>3.0.CO;2-O – volume-title: Chinese Materia Medica Dictionary year: 1977 ident: e_1_2_6_17_2 contributor: fullname: Jiangsu New Medical College – ident: e_1_2_6_7_2 doi: 10.1002/1521-3773(20020215)41:4<646::AID-ANIE646>3.0.CO;2-I – volume: 28 start-page: 409 year: 1986 ident: e_1_2_6_20_2 publication-title: Bull. Bot. contributor: fullname: Li BG – ident: e_1_2_6_4_2 doi: 10.1021/ac049335f – ident: e_1_2_6_5_2 doi: 10.1021/ac070146h – ident: e_1_2_6_6_2 doi: 10.1016/j.bbrc.2006.01.069 – volume: 2 start-page: 25 year: 2004 ident: e_1_2_6_19_2 publication-title: Chin. J. Nat. Med. contributor: fullname: Geng Y – ident: e_1_2_6_14_2 doi: 10.1021/ac010569y – ident: e_1_2_6_12_2 doi: 10.1021/ac801153h – ident: e_1_2_6_13_2 doi: 10.1021/ac034794m – ident: e_1_2_6_21_2 doi: 10.1002/jms.988 |
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Snippet | The chemical diversity of secondary metabolites in medicinal plant makes it a huge challenge to isolate the bioactive compounds from herbal extracts, so quick... Abstract The chemical diversity of secondary metabolites in medicinal plant makes it a huge challenge to isolate the bioactive compounds from herbal extracts,... |
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SubjectTerms | Dioscorea Dioscorea - chemistry Drugs, Chinese Herbal - chemistry Drugs, Chinese Herbal - metabolism Humans Magnetite Nanoparticles - chemistry Molecular Probe Techniques Molecular Probes - chemistry Serum Albumin - chemistry Spectrometry, Mass, Electrospray Ionization - methods Spirostans - chemistry |
Title | Rapid probe and isolation of bioactive compounds from Dioscorea panthaica using human serum albumin functionalized magnetic nano-particles (HSA-MNPs)-based ligand fishing coupled with electrospray ionization mass spectrometry |
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