Conversion of an anti-single-stranded DNA active site to an anti-fluorescein active site through heavy chain complementarity determining region transplantation

• Published in: The Journal of biological chemistry Vol. 269; no. 11; pp. 7934 - 7940
• Main Authors: G A Gulliver, W D Bedzyk, R G Smith, S L Bode, S Y Tetin, E W Voss, Jr
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 18.03.1994
• Subjects: Amino Acid Sequence; Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - metabolism; Base Sequence; Binding Sites, Antibody; DNA Primers; DNA, Single-Stranded - immunology; Fluorescein; Fluoresceins; Immunoglobulin Heavy Chains - biosynthesis; Immunoglobulin Heavy Chains - metabolism; Mice; Mice, Inbred BALB C - immunology; Molecular Sequence Data; Plasmids; Polymerase Chain Reaction; Promoter Regions, Genetic; Recombinant Proteins - biosynthesis; Recombinant Proteins - metabolism; Spectrometry, Fluorescence

Complementarity determining region (CDR) transplant studies were conducted between two monoclonal antibodies of distinctly different specificities (anti-fluorescein monoclonal antibody (mAb) 4-4-20 and anti-single-stranded DNA (ssDNA) mAb 04-01) which possessed nearly identical light chains but diss...