Quercetin Induces Antiproliferative Activity Against Human Hepatocellular Carcinoma (HepG2) Cells by Suppressing Specificity Protein 1 (Sp1)
ABSTRACT Preclinical Research Quercetin, found in red onions and red apple skin can induce apoptosis insome malignant cells. However, the apoptotic effect of quercetin in hepatocellular carcinoma HepG2 cells via regulation of specificity protein 1 (Sp1) has not been studied. Here, we demonstrated th...
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| Published in | Drug development research Vol. 76; no. 1; pp. 9 - 16 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Blackwell Publishing Ltd
01.02.2015
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| Abstract | ABSTRACT
Preclinical Research
Quercetin, found in red onions and red apple skin can induce apoptosis insome malignant cells. However, the apoptotic effect of quercetin in hepatocellular carcinoma HepG2 cells via regulation of specificity protein 1 (Sp1) has not been studied. Here, we demonstrated that quercetin decreased cell growth and induce apoptosis in HepG2 cells via suppression of Sp1 using 3‐(4,5‐dimethyl‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium, inner salt (MTS) assay, 4′,6‐diamidino‐2‐phenylindole (DAPI) staining, Annexin V, and Western blot analysis, an effect that was dose‐ and time‐dependent manner. Treatment of HepG2 cells with quercetin reduced cell growth and induced apoptosis, followed by regulation of Sp1 and Sp1 regulatory protein. Taken together, the results suggest that quercetin can induce apoptotic cell death by regulating cell cycle and suppressing antiapoptotic proteins. Therefore, quercetin may be useful for cancer prevention. Drug Dev Res 76 : 9–16, 2015 |
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| AbstractList | ABSTRACT
Preclinical Research
Quercetin, found in red onions and red apple skin can induce apoptosis insome malignant cells. However, the apoptotic effect of quercetin in hepatocellular carcinoma HepG2 cells via regulation of specificity protein 1 (Sp1) has not been studied. Here, we demonstrated that quercetin decreased cell growth and induce apoptosis in HepG2 cells via suppression of Sp1 using 3‐(4,5‐dimethyl‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium, inner salt (MTS) assay, 4′,6‐diamidino‐2‐phenylindole (DAPI) staining, Annexin V, and Western blot analysis, an effect that was dose‐ and time‐dependent manner. Treatment of HepG2 cells with quercetin reduced cell growth and induced apoptosis, followed by regulation of Sp1 and Sp1 regulatory protein. Taken together, the results suggest that quercetin can induce apoptotic cell death by regulating cell cycle and suppressing antiapoptotic proteins. Therefore, quercetin may be useful for cancer prevention. Drug Dev Res 76 : 9–16, 2015 Preclinical Research Quercetin, found in red onions and red apple skin can induce apoptosis insome malignant cells. However, the apoptotic effect of quercetin in hepatocellular carcinoma HepG2 cells via regulation of specificity protein 1 (Sp1) has not been studied. Here, we demonstrated that quercetin decreased cell growth and induce apoptosis in HepG2 cells via suppression of Sp1 using 3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay, 4',6-diamidino-2-phenylindole (DAPI) staining, Annexin V, and Western blot analysis, an effect that was dose- and time-dependent manner. Treatment of HepG2 cells with quercetin reduced cell growth and induced apoptosis, followed by regulation of Sp1 and Sp1 regulatory protein. Taken together, the results suggest that quercetin can induce apoptotic cell death by regulating cell cycle and suppressing antiapoptotic proteins. Therefore, quercetin may be useful for cancer prevention. Drug Dev Res 76 : 9-16, 2015. Preclinical Research Quercetin, found in red onions and red apple skin can induce apoptosis insome malignant cells. However, the apoptotic effect of quercetin in hepatocellular carcinoma HepG2 cells via regulation of specificity protein 1 (Sp1) has not been studied. Here, we demonstrated that quercetin decreased cell growth and induce apoptosis in HepG2 cells via suppression of Sp1 using 3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay, 4',6-diamidino-2-phenylindole (DAPI) staining, Annexin V, and Western blot analysis, an effect that was dose- and time-dependent manner. Treatment of HepG2 cells with quercetin reduced cell growth and induced apoptosis, followed by regulation of Sp1 and Sp1 regulatory protein. Taken together, the results suggest that quercetin can induce apoptotic cell death by regulating cell cycle and suppressing antiapoptotic proteins. Therefore, quercetin may be useful for cancer prevention. Drug Dev Res 76 : 9-16, 2015 |
| Author | Cho, Jung-Jae Bang, Woong Choi, Nag-Jin Seo, Kang Seok Cho, Jin Hyoung Jeon, Young-Joo Shim, Jung-Hyun Lee, Ra Ham Chae, Jung-Il |
| Author_xml | – sequence: 1 givenname: Ra Ham surname: Lee fullname: Lee, Ra Ham organization: Department of Oral Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK21 plus, Chonbuk National University, 651-756, Jeonju – sequence: 2 givenname: Jin Hyoung surname: Cho fullname: Cho, Jin Hyoung organization: Department of Oral Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK21 plus, Chonbuk National University, 651-756, Jeonju – sequence: 3 givenname: Young-Joo surname: Jeon fullname: Jeon, Young-Joo organization: Department of Oral Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK21 plus, Chonbuk National University, 651-756, Jeonju – sequence: 4 givenname: Woong surname: Bang fullname: Bang, Woong organization: Department of Oral Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK21 plus, Chonbuk National University, 651-756, Jeonju – sequence: 5 givenname: Jung-Jae surname: Cho fullname: Cho, Jung-Jae organization: Department of Pharmacy, Natural Medicine Research Institute, College of Pharmacy, Mokpo National University, Jeonnam, 534-729, Muan-gun – sequence: 6 givenname: Nag-Jin surname: Choi fullname: Choi, Nag-Jin organization: Department of Animal Science, College of Agricultural & Life Science, Chonbuk National University, 651-756, Jeonju – sequence: 7 givenname: Kang Seok surname: Seo fullname: Seo, Kang Seok organization: Department of Animal Science and Technology, Sunchon National University, Suncheon, Republic of Korea – sequence: 8 givenname: Jung-Hyun surname: Shim fullname: Shim, Jung-Hyun email: jichae@jbnu.ac.kr organization: Department of Pharmacy, Natural Medicine Research Institute, College of Pharmacy, Mokpo National University, Jeonnam, 534-729, Muan-gun – sequence: 9 givenname: Jung-Il surname: Chae fullname: Chae, Jung-Il email: jichae@jbnu.ac.kr organization: Department of Oral Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK21 plus, Chonbuk National University, 651-756, Jeonju |
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| Keywords | apoptosis quercetin antiproliferative specificity protein 1 human hepatocellular carcinoma |
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Preclinical Research
Quercetin, found in red onions and red apple skin can induce apoptosis insome malignant cells. However, the apoptotic effect of... Preclinical Research Quercetin, found in red onions and red apple skin can induce apoptosis insome malignant cells. However, the apoptotic effect of quercetin... |
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| SubjectTerms | antiproliferative Apoptosis Cell cycle Cell growth human hepatocellular carcinoma Liver cancer Proteins quercetin specificity protein 1 |
| Title | Quercetin Induces Antiproliferative Activity Against Human Hepatocellular Carcinoma (HepG2) Cells by Suppressing Specificity Protein 1 (Sp1) |
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