Fibroblast growth factor-binding protein facilitates the growth and migration of skin-derived precursors
Fibroblast growth factors (FGFs) are important regulators of cell proliferation, migration, and differentiation during wound healing. FGF-binding protein (FGF-BP) plays a critical role in activating FGFs by releasing them from the extracellular matrix. Although previous studies have demonstrated a p...
Saved in:
Published in | Journal of cutaneous medicine and surgery Vol. 15; no. 4; p. 201 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.07.2011
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Fibroblast growth factors (FGFs) are important regulators of cell proliferation, migration, and differentiation during wound healing. FGF-binding protein (FGF-BP) plays a critical role in activating FGFs by releasing them from the extracellular matrix. Although previous studies have demonstrated a pivotal role for FGF-BP in wound healing and angiogenesis, little is known about the biologic effects of FGF-BP on skin stem cells that contribute to wound healing.
To investigate the effects of FGF-BP on the growth and migration of skin-derived precursors (SKPs).
FGF-BP was titrated to determine the optimal concentration that maximally stimulated cell proliferation. Cellular phenotype and telomerase activity were compared in the presence and absence of FGF-BP. The effect of FGF-BP on cell migration was observed by intravenously transplanting SKPs to adult mice.
Cell proliferation was maximally stimulated by FGF-BP at a concentration of 10 ng/mL without changing the intrinsic characteristics of SKPs. Low levels of telomerase activity were detected, and FGF-BP decreased the rate at which telomerase activity was downregulated. In vivo, FGF-BP remarkably enhanced the migration of SKPs to skin lesion sites.
FGF-BP exerts a positive effect on the growth and migration of SKPs, suggesting a potential role for SKPs in wound healing. |
---|---|
AbstractList | Fibroblast growth factors (FGFs) are important regulators of cell proliferation, migration, and differentiation during wound healing. FGF-binding protein (FGF-BP) plays a critical role in activating FGFs by releasing them from the extracellular matrix. Although previous studies have demonstrated a pivotal role for FGF-BP in wound healing and angiogenesis, little is known about the biologic effects of FGF-BP on skin stem cells that contribute to wound healing.
To investigate the effects of FGF-BP on the growth and migration of skin-derived precursors (SKPs).
FGF-BP was titrated to determine the optimal concentration that maximally stimulated cell proliferation. Cellular phenotype and telomerase activity were compared in the presence and absence of FGF-BP. The effect of FGF-BP on cell migration was observed by intravenously transplanting SKPs to adult mice.
Cell proliferation was maximally stimulated by FGF-BP at a concentration of 10 ng/mL without changing the intrinsic characteristics of SKPs. Low levels of telomerase activity were detected, and FGF-BP decreased the rate at which telomerase activity was downregulated. In vivo, FGF-BP remarkably enhanced the migration of SKPs to skin lesion sites.
FGF-BP exerts a positive effect on the growth and migration of SKPs, suggesting a potential role for SKPs in wound healing. |
Author | Qi, Shao-Hai Xie, Ju-Lin Xu, Ying-Bin Huang, Yong Chen, Lei Liu, Xu-Sheng Shu, Bin |
Author_xml | – sequence: 1 givenname: Yong surname: Huang fullname: Huang, Yong organization: Department of Burns and Emergency Surgery, Sun Yat-sen University, Guangzhou, People's Republic of China – sequence: 2 givenname: Shao-Hai surname: Qi fullname: Qi, Shao-Hai – sequence: 3 givenname: Bin surname: Shu fullname: Shu, Bin – sequence: 4 givenname: Lei surname: Chen fullname: Chen, Lei – sequence: 5 givenname: Ju-Lin surname: Xie fullname: Xie, Ju-Lin – sequence: 6 givenname: Ying-Bin surname: Xu fullname: Xu, Ying-Bin – sequence: 7 givenname: Xu-Sheng surname: Liu fullname: Liu, Xu-Sheng |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21781626$$D View this record in MEDLINE/PubMed |
BookMark | eNo1T0tPxCAY5LDGfejZm-EPdAUKpT2ajasmm3jR8wbKR4u20ACr8d9bo3uaybySWaOFDx4QuqFky0pK7qQUMyOUbikhvFmgFWWkLLgUfInWKb0TMpuCX6Ilo7KmFatWqN87HYMeVMq4i-Er99iqNodYaOeN8x2eYsjg_K_sBpdVhoRzD-e08gaProsqu-BxsDh9OF8YiO4TzFyG9hRTiOkKXVg1JLj-xw162z-87p6Kw8vj8-7-ULRlQ3JhlS2bigioGOMCqAJVN3VFOWgt2hJMI2RDNHDLaS2FkSWXprJgycyEEWyDbv92p5MewRyn6EYVv4_ny-wHY0laSg |
CitedBy_id | crossref_primary_10_1186_s13287_020_01841_1 crossref_primary_10_1002_cbin_10717 crossref_primary_10_1515_biol_2021_0128 crossref_primary_10_1111_ics_12587 crossref_primary_10_3390_ijms25137109 crossref_primary_10_1002_ppap_201500162 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.2310/7750.2011.10049 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Medicine |
ExternalDocumentID | 21781626 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- -TM .86 0R~ 1SB 29K 2P1 36B 4.4 53G 54M 5GY 5VS 6NX 78A AABMB AACMV AADUE AAEWN AAGGD AAGMC AAIAL AAJPV AAKGS AANSI AAOVH AAPEO AARDL AARIX AATAA AATBZ AAUAS ABAWP ABCCA ABDWY ABEIX ABFWQ ABHKI ABKRH ABLUO ABPNF ABQKF ABQNX ABQXT ABRHV ABVFX ABYTW ACARO ACDSZ ACDXX ACFEJ ACGBL ACGFS ACGZU ACJER ACJTF ACLFY ACOFE ACOXC ACROE ACSIQ ACTQU ACUAV ACUIR ACXKE ACXMB ADBBV ADEIA ADINQ ADQRH ADRRZ ADTBJ ADUKL ADZZY AECGH AENEX AEPTA AEQLS AESZF AEUHG AEUIJ AEWDL AEWHI AEXNY AFEET AFGYO AFKRG AFMOU AFQAA AFUIA AGKLV AGNHF AGWFA AHBYD AHMBA AHSBF AIIQI AIOMO AJEFB AJUZI AJXAJ ALKWR ALMA_UNASSIGNED_HOLDINGS ALTZF AMCVQ AMKLP ANDLU ARTOV AUTPY AYAKG B8M BBRGL BDDNI BGNMA BKIIM BKSCU BPACV BSEHC BWJAD C45 CAG CBRKF CDWPY CFDXU CGR COF CORYS CUY CVF D-I DC- DC. DL5 DOPDO DU5 DV7 EBD EBS ECM EIF EJD EMB EMOBN F5P FHBDP GROUPED_SAGE_PREMIER_JOURNAL_COLLECTION H13 HG6 HZ~ I09 IHE IL9 J8X JCYGO K.F KDC LAS M4V M4Y NPM NU0 O9- OVD PQQKQ Q1R QOS ROL RPX RSV SCNPE SDH SFC SHG SMD SPQ SPV SV3 TEORI U2A VC2 WJK WK8 ZONMY ZPPRI ZRKOI ZSSAH |
ID | FETCH-LOGICAL-c390t-faf39605e62245e1aea898614ebb5c3ed95790be4f41875d7347d6fef07345d52 |
ISSN | 1203-4754 |
IngestDate | Sat Sep 28 07:48:21 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c390t-faf39605e62245e1aea898614ebb5c3ed95790be4f41875d7347d6fef07345d52 |
PMID | 21781626 |
ParticipantIDs | pubmed_primary_21781626 |
PublicationCentury | 2000 |
PublicationDate | 2011-07-01 |
PublicationDateYYYYMMDD | 2011-07-01 |
PublicationDate_xml | – month: 07 year: 2011 text: 2011-07-01 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Journal of cutaneous medicine and surgery |
PublicationTitleAlternate | J Cutan Med Surg |
PublicationYear | 2011 |
SSID | ssj0001154 |
Score | 1.9710847 |
Snippet | Fibroblast growth factors (FGFs) are important regulators of cell proliferation, migration, and differentiation during wound healing. FGF-binding protein... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 201 |
SubjectTerms | Analysis of Variance Animals Carrier Proteins - pharmacology Cell Differentiation - drug effects Cell Movement - drug effects Cell Proliferation - drug effects Enzyme-Linked Immunosorbent Assay Fibroblast Growth Factor 2 - metabolism Flow Cytometry Mice Mice, Inbred BALB C Mice, Nude Multipotent Stem Cells - drug effects Multipotent Stem Cells - transplantation Phenotype Skin - cytology Skin - metabolism Telomerase - metabolism Wound Healing - drug effects |
Title | Fibroblast growth factor-binding protein facilitates the growth and migration of skin-derived precursors |
URI | https://www.ncbi.nlm.nih.gov/pubmed/21781626 |
Volume | 15 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1NT9wwELUWkKpeKqAfQAH50FvlNrux4-RYIdAKARdAoieUOGM2qtggyPbAb-BHM2M72bC0VeESrWyv5c17a78Ze8aMfVGJAZlHkcgyHQtZQCmy3KZCaVmaEZi4kBTvfHySjM_l4YW6GAweeqeWZk3xzdz_Ma7kNahiGeJKUbIvQLbrFAvwM-KLT0QYn_-F8QHaunWB-rf5eoXmdDMJ1-eQuVv6MPOabrOkYp-NmxI6IDFCa3KaX1dXt51svPtVTUWJQ_8NLn2AmaEeD_s9zxWsmaGyBDpD2-7Qux7vepHWjjDBJf2zDqsk-VndIYLTSV6LcV51bp7JzPGt6hi7F4JHjqDq-yfI4apb_4SfUkcRkkD7VNHdnKt63JL9CdR_dXFiJxWKaGjUNz7tKiW6y_otEZmba4czGlnpMPFx-P-uXci03VYtsSWd0mx5Qp6fsKpT2iIy3ttf49NE0bi-L4zK5Zf2PS3YKk6znK2ydwEq_sMzZ40NYLrO3hwHsN6zyZxA3FOCPyUQDwTiPQJxJFDbGuHmHYF4bXmfQHxOoA_s_GD_bG8swtUbwsRZ1Aib2xhtWwUJSjwFwxzyNEtRykFRKBNDSbu7UQHSyiFavKWOpS4TCxZXDKlKNfrIlqf1FDYYx1IZgxxq7ENqq3OtwYxQBhulsdN4k33y7-jyxudXuWzf3tZfaz6zt3OqbbMVi39o2EF12BS7DrZH-TdlwA |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Fibroblast+growth+factor-binding+protein+facilitates+the+growth+and+migration+of+skin-derived+precursors&rft.jtitle=Journal+of+cutaneous+medicine+and+surgery&rft.au=Huang%2C+Yong&rft.au=Qi%2C+Shao-Hai&rft.au=Shu%2C+Bin&rft.au=Chen%2C+Lei&rft.date=2011-07-01&rft.issn=1203-4754&rft.volume=15&rft.issue=4&rft.spage=201&rft_id=info:doi/10.2310%2F7750.2011.10049&rft_id=info%3Apmid%2F21781626&rft_id=info%3Apmid%2F21781626&rft.externalDocID=21781626 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1203-4754&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1203-4754&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1203-4754&client=summon |