Biology of the Porifera: cell culture
The discovery that dissociated sponge cells will reaggregate to form a functional organism was the basis for the establishment of sponge cell cultures that have been used as a model for the study of fundamental processes in developmental biology and immunology. More recent is the discovery of unique...
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| Published in | Canadian journal of zoology Vol. 84; no. 2; pp. 167 - 174 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
Ottawa, Canada
NRC Research Press
01.02.2006
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The discovery that dissociated sponge cells will reaggregate to form a functional organism was the basis for the establishment of sponge cell cultures that have been used as a model for the study of fundamental processes in developmental biology and immunology. More recent is the discovery of unique bioactive compounds in marine sponges, and the feasibility of in vitro production of these chemicals is being evaluated. Techniques are well established for cell dissociation; development of several nutrient media formulations has resulted in improvements in viability and cell division; and molecular approaches to identification of genes responsible for regulation of cell cycling may provide unique perspectives in culture optimization. The use of novel substrates for immobilization of cells offers alternatives for proliferation and scale-up. All of these results support the potential for development of a model system for the study of basic metabolic processes involved in cell differentiation, as well as an in vitro production system for sponge-derived bioactive compounds. Perhaps more important, however, is the development of cell lines of these "simple" metazoans to facilitate basic cell physiology and molecular biology research that may be applied to understanding more complex metazoan systems, including humans. |
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| AbstractList | The discovery that dissociated sponge cells will reaggregate to form a functional organism was the basis for the establishment of sponge cell cultures that have been used as a model for the study of fundamental processes in developmental biology and immunology. More recent is the discovery of unique bioactive compounds in marine sponges, and the feasibility of in vitro production of these chemicals is being evaluated. Techniques are well established for cell dissociation; development of several nutrient media formulations has resulted in improvements in viability and cell division; and molecular approaches to identification of genes responsible for regulation of cell cycling may provide unique perspectives in culture optimization. The use of novel substrates for immobilization of cells offers alternatives for proliferation and scale-up. All of these results support the potential for development of a model system for the study of basic metabolic processes involved in cell differentiation, as well as an in vitro production system for sponge-derived bioactive compounds. Perhaps more important, however, is the development of cell lines of these "simple" metazoans to facilitate basic cell physiology and molecular biology research that may be applied to understanding more complex metazoan systems, including humans. The discovery that dissociated sponge cells will reaggregate to form a functional organism was the basis for the establishment of sponge cell cultures that have been used as a model for the study of fundamental processes in developmental biology and immunology. More recent is the discovery of unique bioactive compounds in marine sponges, and the feasibility of in vitro production of these chemicals is being evaluated. Techniques are well established for cell dissociation; development of several nutrient media formulations has resulted in improvements in viability and cell division; and molecular approaches to identification of genes responsible for regulation of cell cycling may provide unique perspectives in culture optimization. The use of novel substrates for immobilization of cells offers alternatives for proliferation and scale-up. All of these results support the potential for development of a model system for the study of basic metabolic processes involved in cell differentiation, as well as an in vitro production system for sponge-derived bioactive compounds. Perhaps more important, however, is the development of cell lines of these "simple" metazoans to facilitate basic cell physiology and molecular biology research that may be applied to understanding more complex metazoan systems, including humans.Original Abstract: la decouverte que des cellules dissociees d'eponge se regroupent pour former un organisme fonctionnel est la base de l'etablissement des cultures de cellules d'eponges utilisees pour l'etude des processus fondamentaux de biologie du developpement et d'immunologie. Plus recemment, apres la decouverte de composes bioactifs exceptionnels chez les eponges marines, on est en train d'evaluer la possibilite de produire ces composes chimiques in vitro. Les techniques de dissociation cellulaire sont bien rodees; la mise au point de plusieurs formules de milieux nutritifs a ameliore la viabilite et la division cellulaire; les techniques moleculaires d'identification des genes responsables des cycles cellulaires ouvriront peut-etre des perspectives inedites sur l'optimisation des cultures. L'utilisation de nouveaux substrats pour l'immobilisation des cellules offre des solutions de rechange pour la proliferation et le passage a une echelle de production superieure. Tous ces resultats augmentent la possibilite de developper un systeme modele pour etudier les processus metaboliques de base impliques dans la differentiation cellulaire et aussi de mettre en operation un systeme in vitro pour la production des composes bioactifs derives des eponges. Cependant, il est sans doute plus important d'isoler des lignees cellulaires de ces metazoaires ' simples ' pour des etudes de la physiologie cellulaire et de la biologie moleculaire, dont les resultats serviront a comprendre les systemes plus complexes des metazoaires, y compris ceux des humains.[Traduit par la Redaction] |
| Abstract_FL | La découverte que des cellules dissociées d'éponge se regroupent pour former un organisme fonctionnel est la base de l'établissement des cultures de cellules d'éponges utilisées pour l'étude des processus fondamentaux de biologie du développement et d'immunologie. Plus récemment, après la découverte de composés bioactifs exceptionnels chez les éponges marines, on est en train d'évaluer la possibilité de produire ces composés chimiques in vitro. Les techniques de dissociation cellulaire sont bien rôdées; la mise au point de plusieurs formules de milieux nutritifs a amélioré la viabilité et la division cellulaire; les techniques moléculaires d'identification des gènes responsables des cycles cellulaires ouvriront peut-être des perspectives inédites sur l'optimisation des cultures. L'utilisation de nouveaux substrats pour l'immobilisation des cellules offre des solutions de rechange pour la prolifération et le passage à une échelle de production supérieure. Tous ces résultats augmentent la possibilité de développer un système modèle pour étudier les processus métaboliques de base impliqués dans la différentiation cellulaire et aussi de mettre en opération un système in vitro pour la production des composés bioactifs dérivés des éponges. Cependant, il est sans doute plus important d'isoler des lignées cellulaires de ces métazoaires « simples » pour des études de la physiologie cellulaire et de la biologie moléculaire, dont les résultats serviront à comprendre les systèmes plus complexes des métazoaires, y compris ceux des humains.[Traduit par la Rédaction] |
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| Author | Pomponi, S A |
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