A method for the determination of glucose synthesis in isolated bovine hepatocytes

• Published in: The Journal of nutritional biochemistry Vol. 10; no. 4; pp. 205 - 209
• Main Authors: Azain, M.J, Kasser, T.R, Baile, C.A
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.04.1999; Elsevier Science
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; gluconeogenesis; hepatocytes; Liver. Bile. Biliary tracts; metabolism; Vertebrates: digestive system; gluconeogenesis; metabolism; hepatocytes; Methodology; Bovine; Digestive system; Liver; Biosynthesis; Glucose; Radioisotope; Metabolism; Vertebrata; Mammalia; Analysis method; Hepatocyte; Animal; Carbohydrate; Biosynthesis precursor; Artiodactyla; Ungulata; Gluconeogenesis

A simple method for determining glucose synthesis from radiolabeled precursors in isolated bovine hepatocytes using ion exchange resins is presented. This method allows processing of multiple small volume samples using suspensions of anion and cation exchange resins rather than traditional stacked c...