Cecal ligation and double puncture impairs heat shock protein 70 (HSP-70) expression in the lungs of rats

• Published in: Shock (Augusta, Ga.) Vol. 13; no. 1; p. 19
• Main Authors: Weiss, Y G, Bouwman, A, Gehan, B, Schears, G, Raj, N, Deutschman, C S
• Format: Journal Article
• Language: English
• Published: United States 01.01.2000
• Subjects: Animals; Cecum; Disease Models, Animal; Gene Expression Regulation; HSP70 Heat-Shock Proteins - biosynthesis; HSP70 Heat-Shock Proteins - genetics; Lung - metabolism; Lung - physiopathology; Male; Peritonitis - genetics; Peritonitis - physiopathology; Punctures; Rats; Rats, Sprague-Dawley; Respiratory Distress Syndrome, Adult - etiology; Respiratory Distress Syndrome, Adult - genetics; Respiratory Distress Syndrome, Adult - physiopathology; Sepsis - genetics; Sepsis - physiopathology
• ISSN: 1073-2322; 1540-0514
• DOI: 10.1097/00024382-200013010-00004

Induction of the heat shock response may improve outcome from pathophysiological disturbances. This improvement is associated with and believed to result from expression of heat shock protein (HSP)-70. Therefore, we examined the temporal expression of HSP-70 in an animal model of acute respiratory distress syndrome (ARDS) secondary to fecal peritonitis. Specifically, we hypothesize that sepsis in rats impairs pulmonary HSP-70 expression. ARDS was induced in adolescent rats via cecal ligation and double puncture (2CLP). Sham-operated animals served as controls. Lung tissue was collected 0, 3, 6, 16, 24, and 48 h after 2CLP and sham operation. Northern blot hybridization analysis was performed to detect steady-state HSP-70 messenger ribonucleic (mRNA) levels. HSP-70 protein levels were determined via immunoblotting and immunohistochemistry. Mortality after 2CLP was 50% at 24 h and 75% at 48 h. Northern blot hybridization analysis revealed no significant change in steady-state HSP-70 mRNA levels in lung at any time after 2CLP. HSP-70 steady-state mRNA levels increased after sham operation and was higher than values in 2CLP at 6, 16, and 24 h. HSP-70 protein levels did not change over time in either group. Thus, the expression of HSP-70 does not change after 2CLP. Although lack of an increase in protein levels may be adaptive after sham operation, it is not appropriate after 2CLP. Therefore, failed HSP-70 expression represents a form of pulmonary epithelial dysfunction that may contribute to lung injury in sepsis.