Comparative study of different fluorescent dyes for the detection of proteins on membranes using the peroxyoxalate chemiluminescent reaction

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 793; no. 1; pp. 75 - 81
• Main Authors: Salerno, Doris, Daban, Joan-Ramon
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.08.2003
• Subjects: Fluorescent Dyes - chemistry; Membranes, Artificial; Oxalates - chemistry; Polyvinyls - chemistry; Proteins; Proteins - analysis; Proteins; Fluorescent dyes; Peroxyoxalate chemiluminescence

We have previously shown that the bis(2,4,6-trichlorophenyl)oxalate (TCPO)–H 2O 2 chemiluminescent reaction in acetone can be used for the detection of proteins labeled with the fluorescent reagent 2-methoxy-2,4-diphenyl-3(2 H)-furanone (MDPF) on polyvinylidene difluoride (PVDF) membranes. To improv...