Intracellular labeling method for chip-based capillary electrophoresis fluorimetric single cell analysis using liposomes

• Published in: Journal of Chromatography A Vol. 1135; no. 1; pp. 109 - 114
• Main Authors: Sun, Yue, Lu, Min, Yin, Xue-Feng, Gong, Xing-Guo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.11.2006; Elsevier
• Subjects: Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Cells - chemistry; Cells - metabolism; Cells - ultrastructure; Chip-based CE; Electrophoresis, Capillary - methods; Fluorescence; Fluorescent Dyes - chemistry; Fluorometry - methods; Fundamental and applied biological sciences. Psychology; Intracellular derivatization; Lasers; LIF; Liposomes; Liposomes - chemistry; Reproducibility of Results; Sensitivity and Specificity; Single cell analysis; Staining and Labeling; LIF; Liposomes; Chip-based CE; Single cell analysis; Intracellular derivatization; Encapsulation; Liver; Confocal microscopy; Derivatization; Scanning microscope; Hepatocyte; Tumor cell; Human; Fluorescence detector; Capillary electrophoresis; Laser induced fluorescence; Liposome; System on a chip; Cell line; Analysis method; Fluorescent labelling; Intracellular; Miniaturization; Microfluidics; Fluorescence microscopy; Confocal laser scanning microscopy

An intracellular derivatization method mediated by liposome was developed for single cell analysis with chip-based capillary electrophoresis (CE) and laser-induced fluorescence (LIF) detection. Liposomes with an average diameter of 100 nm were produced from phosphatidylcholine to encapsulate fluores...