Characterisation of biocondensate microfluidic flow using array-detector FCS

• Published in: Biochimica et biophysica acta. General subjects Vol. 1868; no. 9; p. 130673
• Main Authors: Dilissen, Stijn, Silva, Pedro L., Smolentseva, Anastasia, Kache, Tom, Thoelen, Ronald, Hendrix, Jelle
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2024
• Subjects: commercialization; condensates; Diffusion; fluorescence; Fluorescence correlation spectroscopy; Humans; Lab-On-A-Chip Devices; Liquid-liquid phase separation; Microfluidic Analytical Techniques - instrumentation; Microfluidic Analytical Techniques - methods; Microfluidics; Microfluidics - methods; organ-on-a-chip; separation; Single-molecule research; Spectrometry, Fluorescence - methods; Tau; tau Proteins - chemistry; tau Proteins - metabolism; Tau; Liquid-liquid phase separation; Single-molecule research; Microfluidics; Fluorescence correlation spectroscopy

Biomolecular condensation via liquid-liquid phase separation (LLPS) is crucial for orchestrating cellular activities temporospatially. Although the rheological heterogeneity of biocondensates and the structural dynamics of their constituents carry critical functional information, methods to quantita...