Quantitative analysis of hydrophobic pulmonary surfactant proteins by high-performance liquid chromatography with light-scattering detection

• Published in: Journal of Chromatography A Vol. 870; no. 1; pp. 363 - 369
• Main Authors: Bünger, H, Kaufner, L, Pison, U
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 18.02.2000; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Fundamental and applied biological sciences. Psychology; Light; Lipids; Miscellaneous; Proteins; Pulmonary Surfactants - analysis; Pulmonary Surfactants - isolation & purification; Scattering, Radiation; Spectrophotometry, Ultraviolet; Surfactant proteins; Proteins; Detection, LC; Surfactant proteins; Lipids; Chemical analysis; Preparative chromatography; Light scattering; Lung; HPLC chromatography; Protein; Gel permeation chromatography; Vertebrata; Mammalia; Ultraviolet detector; Analysis method; Pulmonary surfactant; Animal; Sheep; Artiodactyla; Ungulata; Quantitative analysis

A new method for the separation and quantification of two hydrophobic lung surfactant proteins (SPs) is described. It is based on size-exclusion chromatography using the apolar stationary phase butyl silicagel with a pore size of 30 nm and isocratic elution with chloroform, methanol and trifluoroace...