Allergenicity assessment of Buchanania lanzan protein extract in Balb/c mice

• Published in: International immunopharmacology Vol. 63; pp. 170 - 182
• Main Authors: Kumar, Sachin, Sharma, Akanksha, Gupta, Rinkesh Kumar, Verma, Ajay Kumar, Dwivedi, Premendra D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2018; Elsevier BV
• Subjects: Allergenicity; Allergens - administration & dosage; Allergens - immunology; Allergies; Allergy; Anacardiaceae - immunology; Anaphylaxis; Animals; Binding; Buchanania; Buchanania lanzan; Chymases - blood; Cytokines; Cytokines - blood; Female; Flowers & plants; Food Hypersensitivity - immunology; Food Hypersensitivity - pathology; GATA-3 protein; Histamine; Humans; Immunoblotting; Immunoglobulin E; Immunoglobulin E - blood; Immunoglobulin G; Immunoglobulin G - blood; Infiltration; Intestines - drug effects; Intestines - immunology; Intestines - pathology; Lung - drug effects; Lung - immunology; Lung - pathology; Lymphocytes T; Mice; Mice, Inbred BALB C; Molecular weight; Nuts; Nuts - immunology; Organs; PGD2; Plant Extracts - administration & dosage; Plant Extracts - immunology; Plant Proteins - administration & dosage; Plant Proteins - immunology; Prostaglandin D2 - blood; Proteins; Skin Tests; Spleen - drug effects; Spleen - immunology; Spleen - pathology; Transcription factors; Tree nuts; India; PGD2; Allergy; Histamine; Cytokines; Tree nuts; Buchanania lanzan; PGD
• DOI: 10.1016/j.intimp.2018.07.039
• ISSN: 1567-5769; 1878-1705

Tree nuts are among “Big Eight” and have been reported globally for causing allergy. Buchanania lanzan (Bl) is one of the major tree nuts consumed by Indian population. However, very little is known about B. lanzan's induced allergic manifestation. Therefore, evaluation of it's allergenic potential was undertaken. Bl-crude protein extract sensitized BALB/c mice sera were used to identify the allergic proteins by it's IgE binding capability. The major IgE binding proteins found with molecular weight of 11, 20, 23, 25, 48, 54, and 65 kDa. Specific IgE, specific IgG1, MCPT-1, PGD2 and histamine were assessed in mice sera. Enormous amount of mast cell infiltration was noted in different organs. The levels of Th1/Th2 transcription factors GATA-3, SOCS3 and STAT-6 were found upregulated, whereas T-bet was downregulated. Furthermore, elevated Th1/Th2 cytokine responses were observed in mice sera. All together, these reactions developed systemic anaphylaxis upon Bl-CPE challenge in sensitized BALB/c mice. In order to confirm the evidences obtained from the studies carried out in BALB/c, the investigation was extended to human subjects as well. Control subjects and allergic patients were subjected to skin prick test (SPT). Later sera collected from those positive to SPT along with controls were used for IgE immunoblotting. The study evaluated the allergic manifestation associated with Bl, and identified it's proteins attributing Bl-mediated allergy. This work may help in managing tree nuts mediated allergies especially due to Buchanania lanzan sensitization. [Display omitted] •Prevalence of Bl mediated allergy is increasing due to its excessive usage in food preparations.•Bl-CPE consists of seven IgE binding proteins with M.W. of 11, 20, 23, 25, 48, 54 and 65 kDa.•Bl induced the expression of allergic parameters like IgE, IgG1, TSLP, MCPT-1, Histamine, PGD2 and CysL.•Bl elicits T cells and B cells activation along with the plethora of TH2 cytokines (IL-4, IL-5, and IL-13).•Bl shows cross-reactivity among the tree nuts (cashew, pistachio and peanut).