Cell lines that express membrane-associated DNA for anti-DNA antibody detection

• Published in: Clinical chemistry and laboratory medicine Vol. 46; no. 4; pp. 458 - 462
• Main Authors: Tonutti, Elio, Blasone, Nadia, Visentini, Daniela, Poletto, Monica, Villalta, Danilo, Tozzoli, Renato, Bizzaro, Nicola
• Format: Journal Article
• Language: English
• Published: Berlin Walter de Gruyter 01.01.2008; New York, NY
• Subjects: anti-double-stranded DNA (anti-dsDNA); anti-nucleosome; Antibodies - chemistry; Antibodies, Antinuclear - chemistry; Biological and medical sciences; Blood Chemical Analysis - methods; Cell Line; Cell Line, Tumor; Cell Membrane - metabolism; cell membrane DNA; Chemistry, Clinical - methods; Crithidia; DNA - chemistry; ELISA; Enzyme-Linked Immunosorbent Assay - methods; Fluorescent Antibody Technique, Indirect - methods; General aspects; Humans; indirect immunofluorescence; Investigative techniques, diagnostic techniques (general aspects); Lupus Erythematosus, Systemic - blood; Lupus Erythematosus, Systemic - diagnosis; Medical sciences; Nucleosomes - metabolism; Reproducibility of Results; Sensitivity and Specificity; systemic lupus erythematosus; Immunopathology; Connective tissue disease; Skin disease; cell membrane DNA; Antibody; Nucleosome; Autoimmune disease; Indirect immunofluorescence; Enzyme immunoassay; anti-nucleosome; Medicine; Double stranded DNA; Cell line; Systemic lupus erythematosus; Systemic disease; Clinical biology; Established cell line; Plasma membrane; anti-double-stranded DNA (anti-dsDNA); Diagnosis; Detection; ELISA assay; ELISA

Background: In this study, we evaluated the analytical reliability and clinical sensitivity of two tests (indirect immunofluorescence and ELISA) for anti-DNA antibodies which use cell membrane DNA (cmDNA) as antigen (Wil2 NS lymphoblastoid B cell line). Methods: We tested 97 sera of patients with sy...