Development and Validation of an EI-GC-MS Method for the Determination of Methadone and its Major Metabolites (EDDP and EMDP) in Human Breast Milk

• Published in: Journal of analytical toxicology Vol. 32; no. 7; pp. 478 - 484
• Main Authors: Nikolaou, Panagiota D., Papoutsis, Ioannis I., Maravelias, Constantinos P., Spiliopoulou, Chara A., Pistos, Constantinos M., Calokerinos, Antony C., Atta-Politou, Julia
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.09.2008
• Subjects: Adult; Calibration; Female; Gas Chromatography-Mass Spectrometry; Humans; Indicators and Reagents; Infant, Newborn; Linear Models; Methadone - analysis; Methadone - pharmacokinetics; Milk, Human - chemistry; Narcotics - analysis; Narcotics - pharmacokinetics; Pyrrolidines - analysis; Quality Control; Reference Standards; Reproducibility of Results; Solid Phase Extraction; Spectrometry, Mass, Electrospray Ionization

Methadone is used extensively for the maintenance of opioid-addicted pregnant women. Because methadone and the two major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenylpyrroline (EMDP), are excreted into breast milk, a sensitive and specific ga...