A plant-specific syntaxin-6 protein contributes to the intracytoplasmic route for the begomovirus CabLCV
Because of limited free diffusion in the cytoplasm, viruses must use active transport mechanisms to move intracellularly. Nevertheless, how the plant single-stranded DNA begomoviruses hijack the host intracytoplasmic transport machinery to move from the nucleus to the plasmodesmata remains enigmatic...
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Published in | Plant physiology (Bethesda) Vol. 187; no. 1; pp. 158 - 173 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
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Oxford University Press
04.09.2021
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Abstract | Because of limited free diffusion in the cytoplasm, viruses must use active transport mechanisms to move intracellularly. Nevertheless, how the plant single-stranded DNA begomoviruses hijack the host intracytoplasmic transport machinery to move from the nucleus to the plasmodesmata remains enigmatic. Here, we identified nuclear shuttle protein (NSP)-interacting proteins from Arabidopsis (Arabidopsis thaliana) by probing a protein microarray and demonstrated that the cabbage leaf curl virus NSP, a facilitator of the nucleocytoplasmic trafficking of viral (v)DNA, interacts in planta with an endosomal vesicle-localized, plant-specific syntaxin-6 protein, designated NSP-interacting syntaxin domain-containing protein (NISP). NISP displays a proviral function, unlike the syntaxin-6 paralog AT2G18860 that failed to interact with NSP. Consistent with these findings, nisp-1 mutant plants were less susceptible to begomovirus infection, a phenotype reversed by NISP complementation. NISP-overexpressing lines accumulated higher levels of vDNA than wild-type. Furthermore, NISP interacted with an NSP-interacting GTPase (NIG) involved in NSP-vDNA nucleocytoplasmic translocation. The NISP-NIG interaction was enhanced by NSP. We also showed that endosomal NISP associates with vDNA. NISP may function as a docking site for recruiting NIG and NSP into endosomes, providing a mechanism for the intracytoplasmic translocation of the NSP-vDNA complex toward and from the cell periphery. |
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AbstractList | NISP functions in the intracytoplasmic transport of proteins and DNA from begomoviruses, suggesting a route connecting the nucleocytoplasmic trafficking of viral DNA to the cell surface.
Because of limited free diffusion in the cytoplasm, viruses must use active transport mechanisms to move intracellularly. Nevertheless, how the plant single-stranded DNA begomoviruses hijack the host intracytoplasmic transport machinery to move from the nucleus to the plasmodesmata remains enigmatic. Here, we identified nuclear shuttle protein (NSP)-interacting proteins from Arabidopsis (
Arabidopsis thaliana
) by probing a protein microarray and demonstrated that the cabbage leaf curl virus NSP, a facilitator of the nucleocytoplasmic trafficking of viral (v)DNA, interacts in planta with an endosomal vesicle-localized, plant-specific syntaxin-6 protein, designated NSP-interacting syntaxin domain-containing protein (NISP). NISP displays a proviral function, unlike the syntaxin-6 paralog AT2G18860 that failed to interact with NSP. Consistent with these findings,
nisp-1
mutant plants were less susceptible to begomovirus infection, a phenotype reversed by NISP complementation. NISP-overexpressing lines accumulated higher levels of vDNA than wild-type. Furthermore, NISP interacted with an NSP-interacting GTPase (NIG) involved in NSP–vDNA nucleocytoplasmic translocation. The NISP–NIG interaction was enhanced by NSP. We also showed that endosomal NISP associates with vDNA. NISP may function as a docking site for recruiting NIG and NSP into endosomes, providing a mechanism for the intracytoplasmic translocation of the NSP–vDNA complex toward and from the cell periphery. Because of limited free diffusion in the cytoplasm, viruses must use active transport mechanisms to move intracellularly. Nevertheless, how the plant single-stranded DNA begomoviruses hijack the host intracytoplasmic transport machinery to move from the nucleus to the plasmodesmata remains enigmatic. Here, we identified nuclear shuttle protein (NSP)-interacting proteins from Arabidopsis (Arabidopsis thaliana) by probing a protein microarray and demonstrated that the cabbage leaf curl virus NSP, a facilitator of the nucleocytoplasmic trafficking of viral (v)DNA, interacts in planta with an endosomal vesicle-localized, plant-specific syntaxin-6 protein, designated NSP-interacting syntaxin domain-containing protein (NISP). NISP displays a proviral function, unlike the syntaxin-6 paralog AT2G18860 that failed to interact with NSP. Consistent with these findings, nisp-1 mutant plants were less susceptible to begomovirus infection, a phenotype reversed by NISP complementation. NISP-overexpressing lines accumulated higher levels of vDNA than wild-type. Furthermore, NISP interacted with an NSP-interacting GTPase (NIG) involved in NSP-vDNA nucleocytoplasmic translocation. The NISP-NIG interaction was enhanced by NSP. We also showed that endosomal NISP associates with vDNA. NISP may function as a docking site for recruiting NIG and NSP into endosomes, providing a mechanism for the intracytoplasmic translocation of the NSP-vDNA complex toward and from the cell periphery. |
Author | Fontes, Elizabeth Pacheco Batista Dos Santos, Anésia Aparecida Gouveia-Mageste, Bianca Castro da Silva, José Cleydson Ferreira Dal-Bianco, Maximiller Machado, João Paulo Batista Yazaki, Junshi Kim, Alice Y Martins, Laura Gonçalves Costa Ecker, Joseph R |
AuthorAffiliation | 5 RIKEN Center for Integrative Medical Sciences , Yokohama City, Kanagawa 230-0045, Japan 4 Genomic Analysis Laboratory, Plant Biology Laboratory, Salk Institute for Biological Studies , La Jolla, California 92037, USA 6 Departament of General Biology, Universidade Federal de Viçosa , Viçosa, Minas Gerais 36570-000, Brazil 7 Howard Hughes Medical Institute and Plant Biology Laboratory, The Salk Institute of Biological Studies , La Jolla, California 92037, USA 1 National Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de Viçosa , Viçosa, Minas Gerais 36570-000, Brazil 2 Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa , Viçosa, Minas Gerais 36570-000, Brazil 3 Agronomy Institute, Universidade Federal de Viçosa , Campus Florestal, Florestal, Minas Gerais 35690-000 , Brazil |
AuthorAffiliation_xml | – name: 5 RIKEN Center for Integrative Medical Sciences , Yokohama City, Kanagawa 230-0045, Japan – name: 1 National Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de Viçosa , Viçosa, Minas Gerais 36570-000, Brazil – name: 2 Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa , Viçosa, Minas Gerais 36570-000, Brazil – name: 4 Genomic Analysis Laboratory, Plant Biology Laboratory, Salk Institute for Biological Studies , La Jolla, California 92037, USA – name: 3 Agronomy Institute, Universidade Federal de Viçosa , Campus Florestal, Florestal, Minas Gerais 35690-000 , Brazil – name: 7 Howard Hughes Medical Institute and Plant Biology Laboratory, The Salk Institute of Biological Studies , La Jolla, California 92037, USA – name: 6 Departament of General Biology, Universidade Federal de Viçosa , Viçosa, Minas Gerais 36570-000, Brazil |
Author_xml | – sequence: 1 givenname: Bianca Castro surname: Gouveia-Mageste fullname: Gouveia-Mageste, Bianca Castro organization: National Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-000, Brazil – sequence: 2 givenname: Laura Gonçalves Costa surname: Martins fullname: Martins, Laura Gonçalves Costa organization: National Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-000, Brazil – sequence: 3 givenname: Maximiller surname: Dal-Bianco fullname: Dal-Bianco, Maximiller organization: Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-000, Brazil – sequence: 4 givenname: João Paulo Batista surname: Machado fullname: Machado, João Paulo Batista organization: Agronomy Institute, Universidade Federal de Viçosa, Campus Florestal, Florestal, Minas Gerais 35690-000, Brazil – sequence: 5 givenname: José Cleydson Ferreira surname: da Silva fullname: da Silva, José Cleydson Ferreira organization: National Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-000, Brazil – sequence: 6 givenname: Alice Y surname: Kim fullname: Kim, Alice Y organization: Genomic Analysis Laboratory, Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA – sequence: 7 givenname: Junshi orcidid: 0000-0002-0697-8320 surname: Yazaki fullname: Yazaki, Junshi organization: RIKEN Center for Integrative Medical Sciences, Yokohama City, Kanagawa 230-0045, Japan – sequence: 8 givenname: Anésia Aparecida surname: Dos Santos fullname: Dos Santos, Anésia Aparecida organization: Departament of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-000, Brazil – sequence: 9 givenname: Joseph R orcidid: 0000-0001-5799-5895 surname: Ecker fullname: Ecker, Joseph R organization: Howard Hughes Medical Institute and Plant Biology Laboratory, The Salk Institute of Biological Studies, La Jolla, California 92037, USA – sequence: 10 givenname: Elizabeth Pacheco Batista orcidid: 0000-0002-7986-1369 surname: Fontes fullname: Fontes, Elizabeth Pacheco Batista organization: Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-000, Brazil |
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SubjectTerms | Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis - virology Begomovirus - physiology Cell Nucleus - metabolism |
Title | A plant-specific syntaxin-6 protein contributes to the intracytoplasmic route for the begomovirus CabLCV |
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