Expression of a bacterial catalase in a strictly anaerobic methanogen significantly increases tolerance to hydrogen peroxide but not oxygen

• Published in: Microbiology (Society for General Microbiology) Vol. 160; no. Pt 2; pp. 270 - 278
• Main Authors: Jennings, Matthew E, Schaff, Cody W, Horne, Alexandra J, Lessner, Faith H, Lessner, Daniel J
• Format: Journal Article
• Language: English
• Published: England Society for General Microbiology 01.02.2014
• Subjects: Catalase - biosynthesis; Catalase - genetics; Cell and Molecular Biology of Microbes; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli Proteins - biosynthesis; Escherichia coli Proteins - genetics; Gene Expression; Hydrogen Peroxide - metabolism; Hydrogen Peroxide - toxicity; Methanosarcina - drug effects; Methanosarcina - enzymology; Methanosarcina - genetics; Methanosarcina - metabolism; Microbial Viability - drug effects; Oxygen - metabolism; Oxygen - toxicity; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics

Haem-dependent catalase is an antioxidant enzyme that degrades H2O2, producing H2O and O2, and is common in aerobes. Catalase is present in some strictly anaerobic methane-producing archaea (methanogens), but the importance of catalase to the antioxidant system of methanogens is poorly understood. W...