Enzyme engineering: Reshaping the biocatalytic functions

• Published in: Biotechnology and bioengineering Vol. 117; no. 6; pp. 1877 - 1894
• Main Authors: Ali, Misha, Ishqi, Hassan Mubarak, Husain, Qayyum
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.06.2020
• Subjects: biocatalytic applications; directed evolution; Engineering; enzyme engineering; Enzymes; Immobilization; Protein engineering; Proteins; rational design; semi‐rational design; Stability; Stereoselectivity; Structure-function relationships; rational design; semi-rational design; enzyme engineering; biocatalytic applications; directed evolution

Enzyme engineering is a powerful tool to fine‐tune the enzymes. It is a technique by which the stability, activity, and specificity of the enzymes can be altered. The characteristic properties of an enzyme can be amended by immobilization and protein engineering. Among them, protein engineering is t...