High‐level heterologous production of propionate in engineered Escherichia coli

• Published in: Biotechnology and bioengineering Vol. 117; no. 5; pp. 1304 - 1315
• Main Authors: Miscevic, Dragan, Mao, Ju‐Yi, Moo‐Young, Murray, Chou, Chih‐Hsiung Perry
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.05.2020
• Subjects: Aerobic conditions; Anaerobic conditions; Biosynthesis; Carbon; Coliforms; Cultivation; Deregulation; E coli; Escherichia coli; Fluctuations; Flux; glycerol; Metabolic flux; Metabolism; Metabolites; Mutants; Mutation; Propanol; propionate; Propionic acid; propionyl‐CoA; sleeping beauty mutase; TCA cycle; Tricarboxylic acid cycle; propionyl-CoA; Escherichia coli; TCA cycle; sleeping beauty mutase; glycerol; propionate

A propanologenic (i.e., 1‐propanol‐producing) bacterium Escherichia coli strain was previously derived by activating the genomic sleeping beauty mutase (Sbm) operon. The activated Sbm pathway branches out of the tricarboxylic acid (TCA) cycle at the succinyl‐CoA node to form propionyl‐CoA and its de...