Maturation of peroxisomes in differentiating human hepatoblastoma cells (HepG2): possible involvement of the peroxisome proliferator-activated receptor α (PPARα)

• Published in: Differentiation (London) Vol. 64; no. 1; pp. 55 - 66
• Main Authors: Stier, Harald, Fahimi, H. Dariush, Völkl, Alfred, Baumgart, Eveline, Van Veldhoven, Paul P., Mannaerts, Guy P.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.11.1998; Blackwell Publishing Ltd; Blackwell
• Subjects: Albumins - biosynthesis; Albumins - genetics; Biological and medical sciences; Carcinoma, Hepatocellular - pathology; Cell Differentiation; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Enzyme Induction; Fundamental and applied biological sciences. Psychology; Humans; Liver Neoplasms - pathology; Microbodies - enzymology; Microbodies - ultrastructure; Microscopy, Electron; Molecular and cellular biology; Oxidoreductases - biosynthesis; Oxidoreductases - genetics; Phenotype; Receptors, Cytoplasmic and Nuclear - biosynthesis; Receptors, Cytoplasmic and Nuclear - genetics; Receptors, Cytoplasmic and Nuclear - physiology; RNA, Messenger - biosynthesis; Transcription Factors - biosynthesis; Transcription Factors - genetics; Transcription Factors - physiology; Tumor Cells, Cultured; Human; Cell line; Hepatocyte; Peroxisome; Cell differentiation; In vitro; Peroxisome proliferator

We have studied the alterations of peroxisomes in the human hepatoblastoma cell line HepG2, induced to differentiate by long-term cultivation (20 days without passaging) using morphological and biochemical techniques as well as mRNA analysis. Ultrastructural studies revealed alterations in shape and...