Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community

• Published in: Biotechnology and bioengineering Vol. 117; no. 7; pp. 2008 - 2022
• Main Authors: Wen, Zhiqiang, Ledesma‐Amaro, Rodrigo, Lu, Minrui, Jiang, Yu, Gao, Shuliang, Jin, Mingjie, Yang, Sheng
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.07.2020
• Subjects: adaptive laboratory evolution; Agmatine; Agmatine deiminase; Aldehyde dehydrogenase; Aldehydes; Bioprocessing; Butanol; cell wall lyases; Cell walls; consolidated bioprocessing; Consortia; Evolutionary genetics; Fermentation; Genes; Lignocellulose; low pH resistance; Metabolic engineering; Microbial activity; Microorganisms; pH control; pH effects; Sugar; low pH resistance; consolidated bioprocessing; agmatine deiminase; adaptive laboratory evolution; cell wall lyases

Synthetic microbial communities have become a focus of biotechnological research since they can overcome several of the limitations of single‐specie cultures. A paradigmatic example is Clostridium cellulovorans DSM 743B, which can decompose lignocellulose but cannot produce butanol. Clostridium beij...