ABCG2-associated resistance to Hoechst 33342 and topotecan in a murine cell model with constitutive expression of side population characteristics

• Published in: Cytometry. Part A Vol. 75A; no. 11; pp. 924 - 933
• Main Authors: Smith, Paul J, Furon, Emeline, Wiltshire, Marie, Campbell, Lee, Feeney, Graham P, Snyder, Ronald D, Errington, Rachel J
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.11.2009
• Subjects: ABCG2; Animals; ATP Binding Cassette Transporter, Sub-Family G, Member 2; ATP-Binding Cassette Transporters - metabolism; Benzimidazoles - pharmacology; Cell Line, Tumor; Cell Separation; Cross-Linking Reagents - pharmacology; Distamycins - pharmacology; drug resistance; Enzyme Inhibitors - pharmacology; Flow Cytometry - methods; Fluorescent Dyes - pharmacology; Hoechst 33342; Humans; Ligands; lung cancer; Mice; Nitrogen Mustard Compounds - pharmacology; Polyamines - chemistry; side population; topotecan; Topotecan - pharmacology

Drug resistant tumor "side-populations," enriched in cancer stem cells and identified by reduced accumulation of Hoechst 33342 under ABCG2-mediated efflux, may compromise therapeutic outcome. Side-population cells have predicted resistance to minor groove ligands, including the DNA topoiso...