Simultaneous identification of Fritillariae cirrhosae bulbus and its common adulterants in one reaction by multiplex ligation-dependent probe amplification and high-resolution melting curve assay

• Published in: Gene Vol. 785; p. 145620
• Main Authors: Zhu, Hailan, Wang, Wenbin, Zhou, Yuxin, Wang, Bo, Nie, Jing
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.06.2021
• Subjects: adulterated products; China; cough; cross reaction; detection limit; DNA; DNA Probes; DNA, Plant; Fritillaria - classification; Fritillaria - genetics; Fritillariae cirrhosae bulbus; genes; internal transcribed spacers; medicine; MLPA-HRM; Multiplex Polymerase Chain Reaction - methods; Nucleic Acid Denaturation; prices; Sensitivity and Specificity; Simultaneous identification method; Traditional Chinese medicine; China; HRM; DHPLC; MLPA-HRM; Traditional Chinese medicine; PCR-RFLP; bp; MLPA; ITS1 regions; LHS; NCBI; RHS; Fritillariae cirrhosae bulbus; Simultaneous identification method

•F. cirrhosae bulbus and its adulterants can be identified with two pairs of probes.•This assay is highly sensitive and specific, rapid, simple, and cost-effective.•As low as 10% adulteration can be detected in F. cirrhosae bulbus in one reaction.•Our method can be used as a prototype for detecting...